(Circular) (Six-base) MAPSORT of: pFN21AE9548.seq Check: 8835 from: 1 to: 5899 pFN21AE9548 5899 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2736 2736 Size: 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2868 3242 1937 Size: 931 374 4594 Fragments arranged by size: 4594 931 374 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 3962 5346 5614 1632 Size: 417 1913 1384 268 1917 Fragments arranged by size: 1917 1913 1384 417 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 4798 5775 1203 Size: 3595 977 1327 Fragments arranged by size: 3595 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3059 3091 3059 Size: 32 5867 AlwNI CAG_nnn'CTG Cuts at: 3642 5214 3642 Size: 1572 4327 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 3208 3434 3586 5695 1493 Size: 1715 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 3011 3224 1870 Size: 73 1068 213 4545 Fragments arranged by size: 4545 1068 213 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2534 2711 3220 4675 Size: 325 217 627 282 465 177 509 1455 Cuts at: 4675 618 Size: 1842 Fragments arranged by size: 1842 1455 627 509 465 325 282 217 177 BanII G_rGCy'C Cuts at: 1391 2900 3218 1391 Size: 1509 318 4072 Fragments arranged by size: 4072 1509 318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2571 2759 928 Size: 971 672 188 4068 Fragments arranged by size: 4068 971 672 188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2095 2126 4510 Size: 107 122 71 631 605 423 31 2384 Cuts at: 4510 136 Size: 1525 Fragments arranged by size: 2384 1525 631 605 423 122 107 71 31 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 2072 2694 3960 5116 1790 Size: 282 622 1266 1156 2573 Fragments arranged by size: 2573 1266 1156 622 282 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5899 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3630 4588 442 Size: 416 53 83 399 24 2213 958 1753 Fragments arranged by size: 2213 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2698 4559 1239 Size: 1459 1861 2579 Fragments arranged by size: 2579 1861 1459 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4024 4889 5187 4024 Size: 865 298 4736 Fragments arranged by size: 4736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3690 493 Size: 711 2486 2702 Fragments arranged by size: 2702 2486 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3127 3706 4088 4723 Size: 53 83 186 936 1594 579 382 635 Cuts at: 4723 275 Size: 1451 Fragments arranged by size: 1594 1451 936 635 579 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3059 3089 1178 Size: 30 165 30 1656 30 3988 Fragments arranged by size: 3988 1656 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2576 1186 Size: 1390 4509 BseYI C'CCAG_C Cuts at: 2090 2441 3083 5102 5598 2090 Size: 351 642 2019 496 2391 Fragments arranged by size: 2391 2019 642 496 351 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5326 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3349 4110 5138 1540 Size: 275 186 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2900 3960 4045 5116 2900 Size: 1060 85 1071 3683 Fragments arranged by size: 3683 1071 1060 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2694 2900 3218 3960 4045 5116 Size: 399 282 622 206 318 742 85 1071 Cuts at: 5116 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 622 399 318 282 206 85 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2727 2736 3254 844 Size: 1883 9 518 3489 Fragments arranged by size: 3489 1883 518 9 BsrBI CCG'CTC Cuts at: 1394 1823 2632 3786 1394 Size: 429 809 1154 3507 Fragments arranged by size: 3507 1154 809 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2382 4394 4568 65 Size: 1183 1134 2012 174 1396 Fragments arranged by size: 2012 1396 1183 1134 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4543 4730 1700 Size: 110 2733 187 2869 Fragments arranged by size: 2869 2733 187 110 BsrGI T'GTAC_A Cuts at: 96 2907 96 Size: 2811 3088 BssHII G'CGCG_C Cuts at: 1925 2683 1925 Size: 758 5141 BssSI C'ACGA_G Cuts at: 2600 3040 3959 4971 2600 Size: 440 919 1012 3528 Fragments arranged by size: 3528 1012 919 440 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstXI CCAn_nnnn'nTGG Cuts at: 2548 2764 2548 Size: 216 5683 BstYI r'GATC_y Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894 Size: 1257 367 385 17 1452 86 12 346 Cuts at: 5894 1972 Size: 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2523 2757 2981 513 Size: 552 939 60 459 234 224 3431 Fragments arranged by size: 3431 939 552 459 234 224 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 2694 3415 4209 4229 2694 Size: 721 794 20 4364 Fragments arranged by size: 4364 794 721 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DraIII CAC_nnn'GTG Cuts at: 2544 2602 2544 Size: 58 5841 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2308 2403 Size: 54 1425 50 275 18 312 166 95 Cuts at: 2403 2889 3346 4235 5864 8 Size: 486 457 889 1629 43 Fragments arranged by size: 1629 1425 889 486 457 312 275 166 95 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3346 1537 Size: 275 1534 4090 Fragments arranged by size: 4090 1534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 2885 3829 1353 Size: 989 543 944 3423 Fragments arranged by size: 3423 989 944 543 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4486 4860 5006 387 Size: 1064 138 2897 374 146 1280 Fragments arranged by size: 2897 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2898 2898 Size: 5899 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2698 3962 4559 5346 5614 1239 Size: 393 417 649 1264 597 787 268 1524 Fragments arranged by size: 1524 1264 787 649 597 417 393 268 EcoO109I rG'GnC_Cy Cuts at: 2010 2010 Size: 5899 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 FspI TGC'GCA Cuts at: 2198 4405 2198 Size: 2207 3692 FspAI rTGC'GCAy Cuts at: 2198 2198 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 2234 5046 1678 Size: 556 2812 2531 Fragments arranged by size: 2812 2531 556 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4540 4572 4614 4646 1373 Size: 32 135 32 2968 32 42 32 2626 Fragments arranged by size: 2968 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3049 3243 3489 669 Size: 988 281 1111 194 246 3079 Fragments arranged by size: 3079 1111 988 281 246 194 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5013 5197 1305 Size: 3708 184 2007 Fragments arranged by size: 3708 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2405 2891 10 Size: 54 1425 343 573 486 3018 Fragments arranged by size: 3018 1425 573 486 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 3857 4216 4375 518 Size: 648 1185 1506 359 159 2042 Fragments arranged by size: 2042 1506 1185 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2571 2973 3315 3992 5140 5385 1606 Size: 488 477 402 342 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 488 477 402 342 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2523 2757 2981 513 Size: 552 939 519 234 224 3431 Fragments arranged by size: 3431 939 552 519 234 224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2602 2790 1108 Size: 1494 188 4217 Fragments arranged by size: 4217 1494 188 PfoI T'CCnGG_A Cuts at: 2829 2829 Size: 5899 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3059 3091 3968 4000 5481 5513 3059 Size: 32 877 32 1481 32 3445 Fragments arranged by size: 3445 1481 877 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2010 2010 Size: 5899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspXI vC'TCGA_Gb Cuts at: 3011 3011 Size: 5899 PstI C_TGCA'G Cuts at: 830 2733 3251 830 Size: 1903 518 3478 Fragments arranged by size: 3478 1903 518 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2094 2094 Size: 5899 SacI G_AGCT'C Cuts at: 2900 2900 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SbfI CC_TGCA'GG Cuts at: 2733 3251 2733 Size: 518 5381 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2653 2729 3247 4382 5063 5254 826 Size: 219 1608 76 518 1135 681 191 1471 Fragments arranged by size: 1608 1471 1135 681 518 219 191 76 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 3226 1872 Size: 1354 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3011 4003 4904 5166 820 Size: 197 926 1068 992 901 262 1553 Fragments arranged by size: 1553 1068 992 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5899 StyI C'CwwG_G Cuts at: 513 1065 2004 2448 2523 2757 2981 2997 3338 Size: 552 939 444 75 234 224 16 341 Cuts at: 3338 513 Size: 3074 Fragments arranged by size: 3074 939 552 444 341 234 224 75 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3007 3926 3943 4096 4281 4726 325 Size: 1255 1427 919 17 153 185 445 1498 Fragments arranged by size: 1498 1427 1255 919 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2907 4145 5857 Size: 274 80 33 51 1443 930 1238 1712 Cuts at: 5857 96 Size: 138 Fragments arranged by size: 1712 1443 1238 930 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2122 2521 2545 2548 2764 2098 Size: 24 399 24 3 216 5233 Fragments arranged by size: 5233 399 216 24 24 3 XhoI C'TCGA_G Cuts at: 1943 3011 1943 Size: 1068 4831 XmaI C'CCGG_G Cuts at: 1870 3224 1870 Size: 1354 4545 XmnI GAAnn'nnTTC Cuts at: 2336 4028 2336 Size: 1692 4207 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI