################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII ItaI KasI KpnI KspI MaeII MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 518 GTCTAC GT^[AC][GT]AC AciI 378 CCGC CCGC(-3/-1) Alw44I 713 GTGCAC G^TGCAC ApaLI 713 GTGCAC G^TGCAC Asp700I 730 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 713 GTGCAC G[AT]GC[AT]^C BanI 367 GGTGCC G^G[CT][GA]CC BclI 632 TGATCA T^GATCA BmyI 713 GTGCAC G[AGT]GC[ACT]^C BsaBI 935 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 195 CCAAGG C^C[ACGT][ACGT]GG BsaWI 848 ACCGGA [AT]^CCGG[AT] BsiHKAI 713 GTGCAC G[AT]GC[AT]^C BsiYI 718 CCAGTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 718 CCAGTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 713 GTGCAC G[AGT]GC[ACT]^C BspDI 40 ATCGAT AT^CGAT BspHI 255 TCATGA T^CATGA BstXI 68 CCAGGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 459 GCTTGC GC[ACGT]^[ACGT]GC ClaI 40 ATCGAT AT^CGAT DraI 558 TTTAAA TTT^AAA EarI 121 CTCTTC CTCTTC(1/4) EcoT14I 195 CCAAGG C^C[AT][AT]GG FbaI 632 TGATCA T^GATCA HapII 849 CCGG C^CGG HincII 478 GTTAAC GT[CT]^[GA]AC HindII 478 GTTAAC GT[CT]^[GA]AC HpaI 478 GTTAAC GTT^AAC HpaII 849 CCGG C^CGG Ksp632I 121 CTCTTC CTCTTC(1/4) MamI 935 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 628 CAGCTG C[AC]G^C[GT]G MspI 849 CCGG C^CGG NlaIII 256 CATG CATG^ PleI 303 GACTC GAGTC(4/5) PvuII 628 CAGCTG CAG^CTG RcaI 255 TCATGA T^CATGA SfaNI 958 GCATC GCATC(5/9) SmlI 381 CTTGAG C^T[CT][GA]AG StyI 195 CCAAGG C^C[AT][AT]GG TaqI 41 TCGA T^CGA Tsp45I 400 GTGAC ^GT[GC]AC TthHB8I 41 TCGA T^CGA XmnI 730 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaII 25 GGTCC G^G[AT]CC 658 GGACC BbsI 413 GAAGAC GAAGAC(2/6) 844 GAAGAC BpmI 944 CTCCAG CTGGAG(16/14) 293 CTGGAG BpuAI 413 GAAGAC GAAGAC(2/6) 844 GAAGAC BsrI 662 CCAGT ACTGG(1/-1) 718 CCAGT BstF5I 564 GGATG GGATG(2/0) 708 GGATG Cfr13I 25 GGTCC G^G[ACGT]CC 658 GGACC DdeI 305 CTCAG C^T[ACGT]AG 904 CTGAG FokI 564 GGATG GGATG(9/13) 708 GGATG MaeIII 400 GTGAC ^GT[ACGT]AC 624 GTTAC NdeI 350 CATATG CA^TATG 616 CATATG NlaIV 367 GGTGCC GG[ACGT]^[ACGT]CC 658 GGACCC Sau96I 25 GGTCC G^G[ACGT]CC 658 GGACC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AcsI AfaI AluI ApoI BfaI BssKI BstNI DpnII EcoRII HinfI HphI MaeI MboI MboII MnlI MseI MvaI NdeII RsaI Sau3AI ScrFI TfiI Tru9I Tsp509I