(Circular) (Six-base) MAPSORT of: pFN21AB5752.seq Check: 9584 from: 1 to: 6946 pFN21AB5752 6946 bp With 182 enzymes: SgfI * June 12, 2017 10:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3999 4756 278 Size: 53 83 186 936 2463 757 2468 Fragments arranged by size: 2468 2463 936 757 186 83 53 AccI GT'mk_AC Cuts at: 1937 2859 4289 1937 Size: 922 1430 4594 Fragments arranged by size: 4594 1430 922 Acc65I G'GTAC_C Cuts at: 2427 4267 2427 Size: 1840 5106 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2015 2806 3636 3831 4008 5009 6393 6661 Size: 383 791 830 195 177 1001 1384 268 Cuts at: 6661 1632 Size: 1917 Fragments arranged by size: 1917 1384 1001 830 791 383 268 195 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6749 AflIII A'CryG_T Cuts at: 1203 5845 6822 1203 Size: 4642 977 1327 Fragments arranged by size: 4642 1327 977 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 2824 5675 2824 Size: 2851 4095 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6946 AlwNI CAG_nnn'CTG Cuts at: 2202 3854 3931 3986 4689 6261 2202 Size: 1652 77 55 703 1572 2887 Fragments arranged by size: 2887 1652 1572 703 77 55 ApaI G_GGCC'C Cuts at: 2333 2333 Size: 6946 ApaLI G'TGCA_C Cuts at: 5003 6159 5003 Size: 1156 5790 ApoI r'AATT_y Cuts at: 1493 2987 3253 4094 4255 4481 4633 6742 1493 Size: 1494 266 841 161 226 152 2109 1697 Fragments arranged by size: 2109 1697 1494 841 266 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 2679 2724 3392 3396 3462 4185 5500 Size: 872 1647 45 668 4 66 723 1315 Cuts at: 5500 160 Size: 1606 Fragments arranged by size: 1647 1606 1315 872 723 668 66 45 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2146 2206 2337 4271 1870 Size: 73 192 11 60 131 1934 4545 Fragments arranged by size: 4545 1934 192 131 73 60 11 AvrII C'CTAG_G Cuts at: 3191 3191 Size: 6946 BamHI G'GATC_C Cuts at: 4276 4643 4276 Size: 367 6579 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 2427 3195 4267 5722 Size: 325 217 627 395 245 768 1072 1455 Cuts at: 5722 618 Size: 1842 Fragments arranged by size: 1842 1455 1072 768 627 395 325 245 217 BanII G_rGCy'C Cuts at: 1391 2147 2157 2333 4265 1391 Size: 756 10 176 1932 4072 Fragments arranged by size: 4072 1932 756 176 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2379 3318 3618 928 Size: 971 480 939 300 4256 Fragments arranged by size: 4256 971 939 480 300 BbvCI CC'TCA_GC Cuts at: 3969 3969 Size: 6946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5137 5171 1903 Size: 34 3200 34 3678 Fragments arranged by size: 3678 3200 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4838 6048 4838 Size: 1210 5736 BclI T'GATC_A Cuts at: 1548 4171 1548 Size: 2623 4323 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2328 5557 136 Size: 107 122 71 631 605 656 3229 1525 Fragments arranged by size: 3229 1525 656 631 605 122 107 71 BglII A'GATC_T Cuts at: 2869 6941 2869 Size: 4072 2874 BlpI GC'TnA_GC Cuts at: 4363 4363 Size: 6946 Bme1580I G_kGCm'C Cuts at: 1790 2333 3200 5007 6163 1790 Size: 543 867 1807 1156 2573 Fragments arranged by size: 2573 1807 1156 867 543 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3834 4677 5635 Size: 416 53 83 399 24 2417 843 958 Cuts at: 5635 442 Size: 1753 Fragments arranged by size: 2417 1753 958 843 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6946 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2805 4161 5606 1239 Size: 1566 1356 1445 2579 Fragments arranged by size: 2579 1566 1445 1356 Bpu10I CC'TnA_GC Cuts at: 3969 3969 Size: 6946 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2868 3601 5071 5936 6234 2868 Size: 733 1470 865 298 3580 Fragments arranged by size: 3580 1470 865 733 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5609 882 Size: 617 4110 2219 Fragments arranged by size: 4110 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4737 493 Size: 711 3533 2702 Fragments arranged by size: 3533 2702 711 BsaBI GATnn'nnATC Cuts at: 3921 6940 3921 Size: 3019 3927 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3996 4753 5135 5770 Size: 53 83 186 936 2463 757 382 635 Cuts at: 5770 275 Size: 1451 Fragments arranged by size: 2463 1451 936 757 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3796 5379 5777 6051 6198 6609 3796 Size: 1583 398 274 147 411 4133 Fragments arranged by size: 4133 1583 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2063 2093 2110 2140 2620 Size: 30 165 30 660 30 17 30 480 Cuts at: 2620 2650 2778 2808 1178 Size: 30 128 30 5316 Fragments arranged by size: 5316 660 480 165 128 30 30 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 2411 2510 3171 3299 4158 1186 Size: 1222 3 99 661 128 859 3974 Fragments arranged by size: 3974 1222 859 661 128 99 3 BseYI C'CCAG_C Cuts at: 2297 2789 3199 6149 6645 2297 Size: 492 410 2950 496 2598 Fragments arranged by size: 2950 2598 496 492 410 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6373 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4396 5157 6185 1540 Size: 275 186 2395 761 1028 2301 Fragments arranged by size: 2395 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5007 5092 6163 5007 Size: 85 1071 5790 Fragments arranged by size: 5790 1071 85 BsmI GAATG_Cn' Cuts at: 4457 4550 4457 Size: 93 6853 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2157 2333 3200 4265 5007 5092 Size: 399 357 10 176 867 1065 742 85 Cuts at: 5092 6163 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1065 867 742 399 357 176 85 10 BspEI T'CCGG_A Cuts at: 3796 6609 3796 Size: 2813 4133 BspHI T'CATG_A Cuts at: 3222 4835 6565 3222 Size: 1613 1730 3603 Fragments arranged by size: 3603 1730 1613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4301 844 Size: 3457 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2101 2140 4833 1394 Size: 429 278 39 2693 3507 Fragments arranged by size: 3507 2693 429 278 39 BsrDI GCAATG_nn' Cuts at: 65 1248 5441 5615 65 Size: 1183 4193 174 1396 Fragments arranged by size: 4193 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2185 5590 5777 1700 Size: 110 375 3405 187 2869 Fragments arranged by size: 3405 2869 375 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6946 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 5006 6018 5006 Size: 1012 5934 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6946 BstBI TT'CG_AA Cuts at: 5750 5750 Size: 6946 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6946 BstYI r'GATC_y Cuts at: 1972 2593 2869 2878 2917 4276 4643 5028 5045 Size: 621 276 9 39 1359 367 385 17 Cuts at: 5045 6497 6583 6595 6941 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1359 621 385 367 346 276 86 39 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2004 2114 2543 3961 4068 513 Size: 552 939 110 429 1418 107 3391 Fragments arranged by size: 3391 1418 939 552 429 110 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2903 487 Size: 683 56 1677 4530 Fragments arranged by size: 4530 1677 683 56 BtsI GCAGTG_nn' Cuts at: 4462 5256 5276 4462 Size: 794 20 6132 Fragments arranged by size: 6132 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2628 2663 529 Size: 35 2064 35 4812 Fragments arranged by size: 4812 2064 35 35 DraI TTT'AAA Cuts at: 4250 4250 Size: 6946 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3452 5953 809 Size: 507 2136 2501 1802 Fragments arranged by size: 2501 2136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2352 4393 Size: 54 1425 50 275 18 363 159 2041 Cuts at: 4393 5282 6911 8 Size: 889 1629 43 Fragments arranged by size: 2041 1629 1425 889 363 275 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2397 3603 4876 1353 Size: 1044 1206 1273 3423 Fragments arranged by size: 3423 1273 1206 1044 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2255 5533 5907 6053 387 Size: 1064 138 435 231 3278 374 146 1280 Fragments arranged by size: 3278 1280 1064 435 374 231 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2805 2806 3636 3831 4008 4161 Size: 393 383 790 1 830 195 177 153 Cuts at: 4161 5009 5606 6393 6661 1239 Size: 848 597 787 268 1524 Fragments arranged by size: 1524 848 830 790 787 597 393 383 268 195 177 153 1 EcoO109I rG'GnC_Cy Cuts at: 2329 2329 Size: 6946 EcoRI G'AATT_C Cuts at: 4094 4255 4633 4094 Size: 161 378 6407 Fragments arranged by size: 6407 378 161 FspI TGC'GCA Cuts at: 5452 5452 Size: 6946 HaeII r_GCGC'y Cuts at: 1678 2228 3969 6093 1678 Size: 550 1741 2124 2531 Fragments arranged by size: 2531 2124 1741 550 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 2110 2142 2492 Size: 32 135 32 491 32 15 32 350 Cuts at: 2492 2524 2810 2842 3778 3810 3986 4018 5587 Size: 32 286 32 936 32 176 32 1569 Cuts at: 5587 5619 5661 5693 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1569 936 491 350 286 176 135 42 32 32 32 32 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 3139 3304 4290 4536 669 Size: 988 281 1201 165 986 246 3079 Fragments arranged by size: 3079 1201 988 986 281 246 165 HindIII A'AGCT_T Cuts at: 748 3521 748 Size: 2773 4173 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KpnI G_GTAC'C Cuts at: 2431 4271 2431 Size: 1840 5106 MfeI C'AATT_G Cuts at: 2730 4545 2730 Size: 1815 5131 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2628 6060 6244 1305 Size: 1323 3432 184 2007 Fragments arranged by size: 3432 2007 1323 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5124 Fragments arranged by size: 5124 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 3426 4904 5263 5422 518 Size: 648 1315 945 1478 359 159 2042 Fragments arranged by size: 2042 1478 1315 945 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 2350 3203 4362 5039 6187 6432 1606 Size: 510 234 853 1159 677 1148 245 2120 Fragments arranged by size: 2120 1159 1148 853 677 510 245 234 NaeI GCC'GGC Cuts at: 2187 2187 Size: 6946 NcoI C'CATG_G Cuts at: 513 1065 2004 3961 4068 513 Size: 552 939 1957 107 3391 Fragments arranged by size: 3391 1957 939 552 107 NdeI CA'TA_TG Cuts at: 387 387 Size: 6946 NgoMIV G'CCGG_C Cuts at: 2185 2185 Size: 6946 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6946 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NspI r_CATG'y Cuts at: 1132 1229 2516 4044 4304 5849 1132 Size: 97 1287 1528 260 1545 2229 Fragments arranged by size: 2229 1545 1528 1287 260 97 PacI TTA_AT'TAA Cuts at: 3396 3396 Size: 6946 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6946 PciI A'CATG_T Cuts at: 5845 5845 Size: 6946 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6946 PfoI T'CCnGG_A Cuts at: 2956 2956 Size: 6946 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5015 5047 6528 6560 5015 Size: 32 1481 32 5401 Fragments arranged by size: 5401 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 4516 4516 Size: 6946 PspOMI G'GGCC_C Cuts at: 2329 2329 Size: 6946 PspXI vC'TCGA_Gb Cuts at: 2135 2135 Size: 6946 PstI C_TGCA'G Cuts at: 830 2328 4298 830 Size: 1498 1970 3478 Fragments arranged by size: 3478 1970 1498 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 PvuII CAG'CTG Cuts at: 3203 3203 Size: 6946 SacII CC_GC'GG Cuts at: 2117 2117 Size: 6946 SalI G'TCGA_C Cuts at: 1936 4288 1936 Size: 2352 4594 SbfI CC_TGCA'GG Cuts at: 2328 4298 2328 Size: 1970 4976 ScaI AGT'ACT Cuts at: 3823 5194 6906 3823 Size: 1371 1712 3863 Fragments arranged by size: 3863 1712 1371 SfcI C'TryA_G Cuts at: 826 1045 2324 2856 4294 5429 6110 6301 826 Size: 219 1279 532 1438 1135 681 191 1471 Fragments arranged by size: 1471 1438 1279 1135 681 532 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2328 2328 Size: 6946 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6946 SmaI CCC'GGG Cuts at: 1872 2148 2208 4273 1872 Size: 276 60 2065 4545 Fragments arranged by size: 4545 2065 276 60 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 2883 3616 5050 5951 6213 Size: 197 926 192 748 733 1434 901 262 Cuts at: 6213 820 Size: 1553 Fragments arranged by size: 1553 1434 926 901 748 733 262 197 192 SnaBI TAC'GTA Cuts at: 493 4737 493 Size: 4244 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6946 SphI G_CATG'C Cuts at: 1132 4304 1132 Size: 3172 3774 SrfI GCCC'GGGC Cuts at: 2148 2148 Size: 6946 SspI AAT'ATT Cuts at: 5 52 2906 4870 5 Size: 47 2854 1964 2081 Fragments arranged by size: 2854 2081 1964 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6946 StyI C'CwwG_G Cuts at: 513 1065 2004 2537 3191 3961 4068 4385 513 Size: 552 939 533 654 770 107 317 3074 Fragments arranged by size: 3074 939 770 654 552 533 317 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3604 4973 4990 5143 5328 5773 325 Size: 1255 2024 1369 17 153 185 445 1498 Fragments arranged by size: 2024 1498 1369 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3748 3821 5192 Size: 274 80 33 51 1443 1771 73 1371 Cuts at: 5192 6904 96 Size: 1712 138 Fragments arranged by size: 1771 1712 1443 1371 274 138 80 73 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2778 2810 6906 6938 2778 Size: 32 4096 32 2786 Fragments arranged by size: 4096 2786 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6946 XbaI T'CTAG_A Cuts at: 4282 4282 Size: 6946 XhoI C'TCGA_G Cuts at: 1943 2135 1943 Size: 192 6754 XmaI C'CCGG_G Cuts at: 1870 2146 2206 4271 1870 Size: 276 60 2065 4545 Fragments arranged by size: 4545 2065 276 60 XmnI GAAnn'nnTTC Cuts at: 3267 5075 3267 Size: 1808 5138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3997 4754 276 Size: 53 83 186 936 2463 757 2468 Fragments arranged by size: 2468 2463 936 757 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PmlI PpuMI PsrI RsrII SacI SanDI SapI SexAI SfoI SwaI XcmI