(Circular) (Six-base) MAPSORT of: pFN21AB8536.seq Check: 6016 from: 1 to: 7390 pFN21AB8536 7390 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5200 278 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4733 1937 Size: 2796 4594 Acc65I G'GTAC_C Cuts at: 4711 4711 Size: 7390 AclI AA'CG_TT Cuts at: 1563 5517 5890 1563 Size: 3954 373 3063 Fragments arranged by size: 3954 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2657 3005 3507 5453 6837 7105 1632 Size: 1025 348 502 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 1025 502 348 268 AflII C'TTAA_G Cuts at: 820 1017 3577 3781 820 Size: 197 2560 204 4429 Fragments arranged by size: 4429 2560 204 197 AflIII A'CryG_T Cuts at: 1203 2452 3099 3475 4125 4604 6289 7266 1203 Size: 1249 647 376 650 479 1685 977 1327 Fragments arranged by size: 1685 1327 1249 977 650 647 479 376 AgeI A'CCGG_T Cuts at: 6221 6221 Size: 7390 AhdI GACnn_n'nnGTC Cuts at: 6119 6119 Size: 7390 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7390 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3921 3953 3921 Size: 32 7358 AlwNI CAG_nnn'CTG Cuts at: 2050 2879 3028 4378 5133 6705 2050 Size: 829 149 1350 755 1572 2735 Fragments arranged by size: 2735 1572 1350 829 755 149 ApaI G_GGCC'C Cuts at: 4527 4527 Size: 7390 ApaLI G'TGCA_C Cuts at: 5447 6603 5447 Size: 1156 6234 ApoI r'AATT_y Cuts at: 1493 2206 2551 2736 2848 3746 4441 4667 4699 Size: 713 345 185 112 898 695 226 32 Cuts at: 4699 4925 5077 7186 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 898 713 695 345 226 226 185 152 112 32 AseI AT'TA_AT Cuts at: 160 1032 3384 5944 160 Size: 872 2352 2560 1606 Fragments arranged by size: 2560 2352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 AvaI C'yCGr_G Cuts at: 1870 1943 2625 4715 1870 Size: 73 682 2090 4545 Fragments arranged by size: 4545 2090 682 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3168 3201 3550 3583 3168 Size: 33 349 33 6975 Fragments arranged by size: 6975 349 33 33 BamHI G'GATC_C Cuts at: 2778 4720 5087 2778 Size: 1942 367 5081 Fragments arranged by size: 5081 1942 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4530 4711 6166 618 Size: 325 217 627 2743 181 1455 1842 Fragments arranged by size: 2743 1842 1455 627 325 217 181 BanII G_rGCy'C Cuts at: 1391 2626 4392 4527 4709 1391 Size: 1235 1766 135 182 4072 Fragments arranged by size: 4072 1766 1235 182 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3116 4684 928 Size: 971 1217 1568 3634 Fragments arranged by size: 3634 1568 1217 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5581 5615 1903 Size: 34 3644 34 3678 Fragments arranged by size: 3678 3644 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3169 5282 6492 3169 Size: 2113 1210 4067 Fragments arranged by size: 4067 2113 1210 BclI T'GATC_A Cuts at: 1548 4134 1548 Size: 2586 4804 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2390 6001 136 Size: 107 122 71 631 605 718 3611 1525 Fragments arranged by size: 3611 1525 718 631 605 122 107 71 BglII A'GATC_T Cuts at: 3840 7385 3840 Size: 3545 3845 BlpI GC'TnA_GC Cuts at: 4026 4627 4807 4026 Size: 601 180 6609 Fragments arranged by size: 6609 601 180 Bme1580I G_kGCm'C Cuts at: 1790 2833 4274 4527 5451 6607 1790 Size: 1043 1441 253 924 1156 2573 Fragments arranged by size: 2573 1441 1156 1043 924 253 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4529 5121 6079 Size: 416 53 83 399 24 3112 592 958 Cuts at: 6079 442 Size: 1753 Fragments arranged by size: 3112 1753 958 592 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7390 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4406 4406 Size: 7390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2498 2864 3923 6050 1239 Size: 866 393 366 1059 2127 2579 Fragments arranged by size: 2579 2127 1059 866 393 366 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2297 5515 6380 6678 2297 Size: 3218 865 298 3009 Fragments arranged by size: 3218 3009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3111 3576 6053 882 Size: 617 1612 465 2477 2219 Fragments arranged by size: 2477 2219 1612 617 465 BsaAI yAC'GTr Cuts at: 493 1204 5181 493 Size: 711 3977 2702 Fragments arranged by size: 3977 2702 711 BsaBI GATnn'nnATC Cuts at: 3813 7384 3813 Size: 3571 3819 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5197 5579 6214 275 Size: 53 83 186 936 3664 382 635 1451 Fragments arranged by size: 3664 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5823 6221 6495 6642 7053 5823 Size: 398 274 147 411 6160 Fragments arranged by size: 6160 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7165 Fragments arranged by size: 7165 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2897 4398 1186 Size: 1711 1501 4178 Fragments arranged by size: 4178 1711 1501 BseYI C'CCAG_C Cuts at: 4520 6593 7089 4520 Size: 2073 496 4821 Fragments arranged by size: 4821 2073 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3375 4629 1163 Size: 573 1639 1254 3924 Fragments arranged by size: 3924 1639 1254 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4840 5601 6629 1540 Size: 275 186 2839 761 1028 2301 Fragments arranged by size: 2839 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4392 5451 5536 6607 4392 Size: 1059 85 1071 5175 Fragments arranged by size: 5175 1071 1059 85 BsmI GAATG_Cn' Cuts at: 2659 2686 2749 3002 3954 4456 4901 4994 2659 Size: 27 63 253 952 502 445 93 5055 Fragments arranged by size: 5055 952 502 445 253 93 63 27 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2626 2833 4274 4392 4527 4709 5451 Size: 399 836 207 1441 118 135 182 742 Cuts at: 5451 5536 6607 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1441 1071 836 742 399 207 182 135 118 85 BspEI T'CCGG_A Cuts at: 7053 7053 Size: 7390 BspHI T'CATG_A Cuts at: 3259 3856 3876 4137 4659 5279 7009 3259 Size: 597 20 261 522 620 1730 3640 Fragments arranged by size: 3640 1730 620 597 522 261 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2044 4745 844 Size: 1200 2701 3489 Fragments arranged by size: 3489 2701 1200 BsrBI CCG'CTC Cuts at: 1394 1823 2983 5277 1394 Size: 429 1160 2294 3507 Fragments arranged by size: 3507 2294 1160 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4482 4649 5885 6059 65 Size: 1183 3234 167 1236 174 1396 Fragments arranged by size: 3234 1396 1236 1183 174 167 BsrFI r'CCGG_y Cuts at: 1700 1810 6034 6221 1700 Size: 110 4224 187 2869 Fragments arranged by size: 4224 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3447 96 Size: 3351 4039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7390 BssSI C'ACGA_G Cuts at: 3932 5450 6462 3932 Size: 1518 1012 4860 Fragments arranged by size: 4860 1518 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3296 3644 794 Size: 2502 348 4540 Fragments arranged by size: 4540 2502 348 BstBI TT'CG_AA Cuts at: 2159 6194 2159 Size: 4035 3355 BstEII G'GTnAC_C Cuts at: 1184 2104 1184 Size: 920 6470 BstXI CCAn_nnnn'nTGG Cuts at: 2534 3358 2534 Size: 824 6566 BstYI r'GATC_y Cuts at: 1972 2047 2778 3025 3840 4276 4368 4720 5087 Size: 75 731 247 815 436 92 352 367 Cuts at: 5087 5472 5489 6941 7027 7039 7385 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 815 731 436 385 367 352 346 247 92 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 2043 2808 4002 2043 Size: 765 1194 5431 Fragments arranged by size: 5431 1194 765 BtgI C'CryG_G Cuts at: 513 1065 2070 3438 513 Size: 552 1005 1368 4465 Fragments arranged by size: 4465 1368 1005 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6651 Fragments arranged by size: 6651 683 56 BtsI GCAGTG_nn' Cuts at: 4906 5700 5720 4906 Size: 794 20 6576 Fragments arranged by size: 6576 794 20 ClaI AT'CG_AT Cuts at: 1554 2037 1554 Size: 483 6907 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7355 DraI TTT'AAA Cuts at: 2253 4694 2253 Size: 2441 4949 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2479 3417 6397 809 Size: 507 1163 938 2980 1802 Fragments arranged by size: 2980 1802 1163 938 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3220 4116 4837 Size: 54 1425 50 275 18 1390 896 721 Cuts at: 4837 5726 7355 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1390 896 889 721 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4837 1537 Size: 275 3025 4090 Fragments arranged by size: 4090 3025 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3024 3918 5320 1353 Size: 1671 894 1402 3423 Fragments arranged by size: 3423 1671 1402 894 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2963 5977 6351 6497 387 Size: 1064 138 1374 3014 374 146 1280 Fragments arranged by size: 3014 1374 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4390 4390 Size: 7390 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2498 2657 2864 3005 3507 3923 Size: 393 473 393 159 207 141 502 416 Cuts at: 3923 5453 6050 6837 7105 1239 Size: 1530 597 787 268 1524 Fragments arranged by size: 1530 1524 787 597 502 473 416 393 393 268 207 159 141 EcoNI CCTnn'n_nnAGG Cuts at: 3923 3923 Size: 7390 EcoO109I rG'GnC_Cy Cuts at: 4524 4524 Size: 7390 EcoRI G'AATT_C Cuts at: 4699 5077 4699 Size: 378 7012 EcoRV GAT'ATC Cuts at: 3862 4218 3862 Size: 356 7034 FspI TGC'GCA Cuts at: 5896 5896 Size: 7390 HaeII r_GCGC'y Cuts at: 1678 6537 1678 Size: 4859 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2157 2189 2397 2429 2576 Size: 32 135 32 585 32 208 32 147 Cuts at: 2576 2608 3143 3172 3175 3204 3858 3890 4249 Size: 32 535 29 3 29 654 32 359 Cuts at: 4249 4281 4377 4409 6031 6063 6105 6137 1373 Size: 32 96 32 1622 32 42 32 2626 Fragments arranged by size: 2626 1622 654 585 535 359 208 147 135 96 42 32 32 32 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3312 3559 3718 4734 4980 669 Size: 988 281 1374 247 159 1016 246 3079 Fragments arranged by size: 3079 1374 1016 988 281 247 246 159 HindIII A'AGCT_T Cuts at: 748 2531 748 Size: 1783 5607 HpaI GTT'AAC Cuts at: 3718 4980 3718 Size: 1262 6128 KpnI G_GTAC'C Cuts at: 4715 4715 Size: 7390 MfeI C'AATT_G Cuts at: 4989 4989 Size: 7390 MluI A'CGCG_T Cuts at: 7266 7266 Size: 7390 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3786 6504 6688 1305 Size: 2481 2718 184 2007 Fragments arranged by size: 2718 2481 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4118 10 Size: 54 1425 343 2286 3282 Fragments arranged by size: 3282 2286 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2691 3443 3474 4130 5348 5707 5866 Size: 648 1525 752 31 656 1218 359 159 Cuts at: 5866 518 Size: 2042 Fragments arranged by size: 2042 1525 1218 752 656 648 359 159 31 MspA1I CmG'CkG Cuts at: 1606 2012 2318 4613 4806 5483 6631 6876 1606 Size: 406 306 2295 193 677 1148 245 2120 Fragments arranged by size: 2295 2120 1148 677 406 306 245 193 NcoI C'CATG_G Cuts at: 513 1065 3438 513 Size: 552 2373 4465 Fragments arranged by size: 4465 2373 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7390 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7390 NotI GC'GGCC_GC Cuts at: 4837 4837 Size: 7390 NspI r_CATG'y Cuts at: 1132 1229 2456 3103 3479 4129 4748 6293 1132 Size: 97 1227 647 376 650 619 1545 2229 Fragments arranged by size: 2229 1545 1227 650 647 619 376 97 PasI CC'CwG_GG Cuts at: 1348 2821 1348 Size: 1473 5917 PciI A'CATG_T Cuts at: 2452 3099 3475 4125 6289 2452 Size: 647 376 650 2164 3553 Fragments arranged by size: 3553 2164 650 647 376 PflMI CCAn_nnn'nTGG Cuts at: 1108 3547 4378 1108 Size: 2439 831 4120 Fragments arranged by size: 4120 2439 831 PfoI T'CCnGG_A Cuts at: 3171 3171 Size: 7390 PmeI GTTT'AAAC Cuts at: 4694 4694 Size: 7390 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5459 5491 6972 7004 5459 Size: 32 1481 32 5845 Fragments arranged by size: 5845 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7390 PsiI TTA'TAA Cuts at: 4960 4960 Size: 7390 PspOMI G'GGCC_C Cuts at: 4523 4523 Size: 7390 PstI C_TGCA'G Cuts at: 830 2055 4352 4742 830 Size: 1225 2297 390 3478 Fragments arranged by size: 3478 2297 1225 390 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7390 PvuII CAG'CTG Cuts at: 2012 2318 4613 2012 Size: 306 2295 4789 Fragments arranged by size: 4789 2295 306 SacI G_AGCT'C Cuts at: 4392 4392 Size: 7390 SalI G'TCGA_C Cuts at: 1936 4732 1936 Size: 2796 4594 SapI GCTCTTCn'nnn_ Cuts at: 3024 3024 Size: 7390 SbfI CC_TGCA'GG Cuts at: 4742 4742 Size: 7390 ScaI AGT'ACT Cuts at: 3733 5638 7350 3733 Size: 1905 1712 3773 Fragments arranged by size: 3773 1905 1712 SfcI C'TryA_G Cuts at: 826 1045 2051 2244 4348 4455 4738 5873 6554 Size: 219 1006 193 2104 107 283 1135 681 Cuts at: 6554 6745 826 Size: 191 1471 Fragments arranged by size: 2104 1471 1135 1006 681 283 219 193 191 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7390 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7390 SmaI CCC'GGG Cuts at: 1872 4717 1872 Size: 2845 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 3577 3781 5494 6395 6657 Size: 197 926 333 1301 204 1713 901 262 Cuts at: 6657 820 Size: 1553 Fragments arranged by size: 1713 1553 1301 926 901 333 262 204 197 SnaBI TAC'GTA Cuts at: 493 5181 493 Size: 4688 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7390 SphI G_CATG'C Cuts at: 1132 4748 1132 Size: 3616 3774 SspI AAT'ATT Cuts at: 5 52 3085 5314 5 Size: 47 3033 2229 2081 Fragments arranged by size: 3033 2229 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7390 StyI C'CwwG_G Cuts at: 513 1065 3438 3771 4829 513 Size: 552 2373 333 1058 3074 Fragments arranged by size: 3074 2373 1058 552 333 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3977 4548 5417 5434 5587 5772 6217 Size: 1255 2397 571 869 17 153 185 445 Cuts at: 6217 325 Size: 1498 Fragments arranged by size: 2397 1498 1255 869 571 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3039 3447 3731 Size: 274 80 33 51 1443 1062 408 284 Cuts at: 3731 5636 7348 96 Size: 1905 1712 138 Fragments arranged by size: 1905 1712 1443 1062 408 284 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2763 2795 3353 3385 7350 7382 2763 Size: 32 558 32 3965 32 2771 Fragments arranged by size: 3965 2771 558 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4412 1316 Size: 3096 4294 XbaI T'CTAG_A Cuts at: 4726 4726 Size: 7390 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4517 4542 4517 Size: 25 7365 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7390 XmaI C'CCGG_G Cuts at: 1870 4715 1870 Size: 2845 4545 XmnI GAAnn'nnTTC Cuts at: 2736 5519 2736 Size: 2783 4607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5198 276 Size: 53 83 186 936 3664 2468 Fragments arranged by size: 3664 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI