Restriction map for
pFN21AB8468
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII AccII AccIII AciI AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaII HphI ItaI
KasI KspI MaeII MamI MfeI
MluI MroI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TseI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Acc65I 37 GGTACC G^GTACC
AccI 62 GTAGAC GT^[AC][GT]AC
Asp700I 124 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 37 GGTACC G^GTACC
BalI 682 TGGCCA TGG^CCA
BbsI 194 GTCTTC GAAGAC(2/6)
BclI 630 TGATCA T^GATCA
BglII 508 AGATCT A^GATCT
BmyI 660 GGGCAC G[AGT]GC[ACT]^C
BpuAI 194 GTCTTC GAAGAC(2/6)
BsmAI 380 GAGAC GTCTC(1/5)
Bsp1286I 660 GGGCAC G[AGT]GC[ACT]^C
DdeI 596 CTAAG C^T[ACGT]AG
EaeI 682 TGGCCA [CT]^GGCC[GA]
FbaI 630 TGATCA T^GATCA
HpaI 801 GTTAAC GTT^AAC
KpnI 37 GGTACC GGTAC^C
MluNI 682 TGGCCA TGG^CCA
MscI 682 TGGCCA TGG^CCA
MwoI 583 GCTGAACTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
ScaI 136 AGTACT AGT^ACT
SfaNI 748 GCATC GCATC(5/9)
TaqI 793 TCGA T^CGA
Tsp45I 356 GTCAC ^GT[GC]AC
TspRI 842 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 793 TCGA T^CGA
XbaI 361 TCTAGA T^CTAGA
XcmI 550 CCAGTTGACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 124 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 152 AAATTT [GA]^AATT[CT]
824 GAATTT
AlwI 811 GATCC GGATC(4/5)
255 GGATC
ApoI 152 AAATTT [GA]^AATT[CT]
824 GAATTT
BanI 37 GGTACC G^G[CT][GA]CC
571 GGCACC
BpmI 512 CTCCAG CTGGAG(16/14)
548 CTCCAG
BsaJI 251 CCATGG C^C[ACGT][ACGT]GG
612 CCATGG
BsrI 429 ACTGG ACTGG(1/-1)
550 CCAGT
BstYI 255 GGATCT [GA]^GATC[CT]
508 AGATCT
DsaI 251 CCATGG C^C[GA][CT]GG
612 CCATGG
EcoT14I 251 CCATGG C^C[AT][AT]GG
612 CCATGG
HaeIII 442 GGCC GG^CC
683 GGCC
HincII 553 GTTGAC GT[CT]^[GA]AC
801 GTTAAC
HindII 553 GTTGAC GT[CT]^[GA]AC
801 GTTAAC
MflI 255 GGATCT [GA]^GATC[CT]
508 AGATCT
NcoI 251 CCATGG C^CATGG
612 CCATGG
SfcI 74 CTACAG C^T[GA][CT]AG
337 CTACAG
StyI 251 CCATGG C^C[AT][AT]GG
612 CCATGG
XhoII 255 GGATCT [GA]^GATC[CT]
508 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI BfaI BssKI BstF5I
BstNI DpnII EarI EcoRII FokI
HinfI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
TfiI Tru9I Tsp509I