(Linear) (Six-base) MAPSORT of: KIBB8468.seq  Check: 1030  from: 1  to: 891



KIBB8468.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AccI GT'mk_AC

Cuts at:      0      63     891 
   Size:         63     828
 
Acc65I G'GTAC_C

Cuts at:      0      37     891 
   Size:         37     854
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     280     624     891 
   Size:        181      99     344     267

  Fragments arranged by size: 

                 344     267     181      99
 
ApoI r'AATT_y

Cuts at:      0     152     824     891 
   Size:        152     672      67

  Fragments arranged by size: 

                 672     152      67
 
BanI G'GyrC_C

Cuts at:      0      37     571     891 
   Size:         37     534     320

  Fragments arranged by size: 

                 534     320      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     187     891 
   Size:        187     704
 
BclI T'GATC_A

Cuts at:      0     630     891 
   Size:        630     261
 
BglII A'GATC_T

Cuts at:      0     508     891 
   Size:        508     383
 
Bme1580I G_kGCm'C

Cuts at:      0     664     891 
   Size:        664     227
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     533     891 
   Size:        497      36     358

  Fragments arranged by size: 

                 497     358      36
 
BseYI C'CCAG_C

Cuts at:      0     525     891 
   Size:        525     366
 
Bsp1286I G_dGCh'C

Cuts at:      0     664     891 
   Size:        664     227
 
BstYI r'GATC_y

Cuts at:      0     255     508     891 
   Size:        255     253     383

  Fragments arranged by size: 

                 383     255     253
 
BtgI C'CryG_G

Cuts at:      0     251     612     891 
   Size:        251     361     279

  Fragments arranged by size: 

                 361     279     251
 
EaeI y'GGCC_r

Cuts at:      0     682     891 
   Size:        682     209
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     360     643     891 
   Size:        113     247     283     248

  Fragments arranged by size: 

                 283     248     247     113
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     280     497     533     624     891 
   Size:        181      99     217      36      91     267

  Fragments arranged by size: 

                 267     217     181      99      91      36
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     285     891 
   Size:        285     606
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     775     807     891 
   Size:        775      32      84

  Fragments arranged by size: 

                 775      84      32
 
HincII GTy'rAC

Cuts at:      0     555     803     891 
   Size:        555     248      88

  Fragments arranged by size: 

                 555     248      88
 
HpaI GTT'AAC

Cuts at:      0     803     891 
   Size:        803      88
 
KpnI G_GTAC'C

Cuts at:      0      41     891 
   Size:         41     850
 
MscI TGG'CCA

Cuts at:      0     684     891 
   Size:        684     207
 
NcoI C'CATG_G

Cuts at:      0     251     612     891 
   Size:        251     361     279

  Fragments arranged by size: 

                 361     279     251
 
ScaI AGT'ACT

Cuts at:      0     138     891 
   Size:        138     753
 
SfcI C'TryA_G

Cuts at:      0      74     337     891 
   Size:         74     263     554

  Fragments arranged by size: 

                 554     263      74
 
StyI C'CwwG_G

Cuts at:      0     251     612     891 
   Size:        251     361     279

  Fragments arranged by size: 

                 361     279     251
 
TatI w'GTAC_w

Cuts at:      0     136     293     891 
   Size:        136     157     598

  Fragments arranged by size: 

                 598     157     136
 
XbaI T'CTAG_A

Cuts at:      0     361     891 
   Size:        361     530
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     557     891 
   Size:        557     334
 
XmnI GAAnn'nnTTC

Cuts at:      0     128     891 
   Size:        128     763
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     ApoI     BanI     BbsI     BclI    BglII
 Bme1580I     BpmI    BseYI Bsp1286I    BstYI     BtgI     EaeI     EarI
  Eco57MI     FalI    Hin4I   HincII     HpaI     KpnI     MscI     NcoI
     ScaI     SfcI     StyI     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     KasI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XhoI     XmaI     ZraI