(Circular) (Six-base) MAPSORT of: pFN21AE2299.seq Check: 5355 from: 1 to: 5992 pFN21AE2299 5992 bp With 182 enzymes: SgfI * November 10, 2010 12:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2879 2879 Size: 5992 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2306 2939 3335 1937 Size: 369 633 396 4594 Fragments arranged by size: 4594 633 396 369 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2323 4055 5439 5707 1632 Size: 557 134 1732 1384 268 1917 Fragments arranged by size: 1917 1732 1384 557 268 134 AfeI AGC'GCT Cuts at: 2642 2642 Size: 5992 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 2292 4891 5868 1203 Size: 1089 2599 977 1327 Fragments arranged by size: 2599 1327 1089 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 2462 4721 2462 Size: 2259 3733 AleI CACnn'nnGTG Cuts at: 1166 2790 3164 1166 Size: 1624 374 3994 Fragments arranged by size: 3994 1624 374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AlwNI CAG_nnn'CTG Cuts at: 2982 3139 3735 5307 2982 Size: 157 596 1572 3667 Fragments arranged by size: 3667 1572 596 157 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 3317 1870 Size: 73 1374 4545 Fragments arranged by size: 4545 1374 73 AvrII C'CTAG_G Cuts at: 2423 2423 Size: 5992 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2131 3235 3313 4768 618 Size: 325 217 627 344 1104 78 1455 1842 Fragments arranged by size: 1842 1455 1104 627 344 325 217 78 BanII G_rGCy'C Cuts at: 1391 2454 3203 3311 1391 Size: 1063 749 108 4072 Fragments arranged by size: 4072 1063 749 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2764 928 Size: 971 865 4156 Fragments arranged by size: 4156 971 865 BbvCI CC'TCA_GC Cuts at: 2819 2819 Size: 5992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 2829 1548 Size: 1281 4711 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4603 136 Size: 107 122 71 631 605 2931 1525 Fragments arranged by size: 2931 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 2448 3409 2448 Size: 961 5031 Bme1580I G_kGCm'C Cuts at: 1790 2020 3189 3240 4053 5209 1790 Size: 230 1169 51 813 1156 2573 Fragments arranged by size: 2573 1169 1156 813 230 51 BmgBI CAC'GTC Cuts at: 1318 2295 1318 Size: 977 5015 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2135 3723 4681 Size: 416 53 83 399 24 718 1588 958 Cuts at: 4681 442 Size: 1753 Fragments arranged by size: 1753 1588 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2468 2468 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2201 2834 4652 1239 Size: 918 44 633 1818 2579 Fragments arranged by size: 2579 1818 918 633 44 Bpu10I CC'TnA_GC Cuts at: 2151 2819 2151 Size: 668 5324 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2840 3134 3230 4117 4982 5280 2840 Size: 294 96 887 865 298 3552 Fragments arranged by size: 3552 887 865 298 294 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2558 2606 2798 4655 882 Size: 617 1059 48 192 1857 2219 Fragments arranged by size: 2219 1857 1059 617 192 48 BsaAI yAC'GTr Cuts at: 493 1204 2482 2926 3783 493 Size: 711 1278 444 857 2702 Fragments arranged by size: 2702 1278 857 711 444 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3110 3799 4181 4816 Size: 53 83 186 936 1577 689 382 635 Cuts at: 4816 275 Size: 1451 Fragments arranged by size: 1577 1451 936 689 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2649 4425 4823 5097 5244 5655 2649 Size: 1776 398 274 147 411 2986 Fragments arranged by size: 2986 1776 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5767 Fragments arranged by size: 5767 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2921 1186 Size: 1735 4257 BseYI C'CCAG_C Cuts at: 2085 2102 2607 2643 3024 5195 5691 2085 Size: 17 505 36 381 2171 496 2386 Fragments arranged by size: 2386 2171 505 496 381 36 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3152 3307 1163 Size: 573 1416 155 3848 Fragments arranged by size: 3848 1416 573 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 2454 2856 4053 4138 5209 2157 Size: 297 402 1197 85 1071 2940 Fragments arranged by size: 2940 1197 1071 402 297 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2157 2454 2856 3189 3203 3240 Size: 399 230 137 297 402 333 14 37 Cuts at: 3240 3311 4053 4138 5209 1391 Size: 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 333 297 230 137 85 71 37 14 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 2494 3881 5611 2494 Size: 1387 1730 2875 Fragments arranged by size: 2875 1730 1387 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2763 2879 3347 844 Size: 1919 116 468 3489 Fragments arranged by size: 3489 1919 468 116 BsrBI CCG'CTC Cuts at: 1394 1823 3879 1394 Size: 429 2056 3507 Fragments arranged by size: 3507 2056 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3039 4487 4661 65 Size: 1183 1791 1448 174 1396 Fragments arranged by size: 1791 1448 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4636 4823 1700 Size: 110 2826 187 2869 Fragments arranged by size: 2869 2826 187 110 BsrGI T'GTAC_A Cuts at: 96 2280 96 Size: 2184 3808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 2903 4052 5064 2903 Size: 1149 1012 3831 Fragments arranged by size: 3831 1149 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2401 3177 794 Size: 1607 776 3609 Fragments arranged by size: 3609 1607 776 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2792 3166 2792 Size: 374 5618 BstYI r'GATC_y Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987 Size: 1350 367 385 17 1452 86 12 346 Cuts at: 5987 1972 Size: 1977 Fragments arranged by size: 1977 1452 1350 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5440 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 2075 2797 3508 4302 4322 2075 Size: 722 711 794 20 3745 Fragments arranged by size: 3745 794 722 711 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2732 4999 809 Size: 507 1416 2267 1802 Fragments arranged by size: 2267 1802 1416 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2920 3439 4328 Size: 54 1425 50 275 18 1090 519 889 Cuts at: 4328 5957 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1090 889 519 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 3922 1353 Size: 1058 1511 3423 Fragments arranged by size: 3423 1511 1058 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2968 4579 4953 5099 387 Size: 1064 138 1379 1611 374 146 1280 Fragments arranged by size: 1611 1379 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2452 2452 Size: 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2189 2201 2323 2834 4055 4652 Size: 393 525 32 12 122 511 1221 597 Cuts at: 4652 5439 5707 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1221 787 597 525 511 393 268 122 32 12 EcoNI CCTnn'n_nnAGG Cuts at: 3218 3218 Size: 5992 EcoO109I rG'GnC_Cy Cuts at: 2227 2227 Size: 5992 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 5992 FspI TGC'GCA Cuts at: 2207 4498 2207 Size: 2291 3701 HaeII r_GCGC'y Cuts at: 1678 2634 2644 2967 5139 1678 Size: 956 10 323 2172 2531 Fragments arranged by size: 2531 2172 956 323 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 2448 2480 4633 Size: 32 135 32 770 32 74 32 2153 Cuts at: 4633 4665 4707 4739 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2153 770 135 74 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2307 2940 3336 3582 669 Size: 988 281 369 633 396 246 3079 Fragments arranged by size: 3079 988 633 396 369 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 2479 2679 5106 5290 1305 Size: 1041 133 200 2427 184 2007 Fragments arranged by size: 2427 2007 1041 200 184 133 MscI TGG'CCA Cuts at: 10 64 1489 1832 2922 10 Size: 54 1425 343 1090 3080 Fragments arranged by size: 3080 1425 1090 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2790 3164 3233 3269 3950 4309 4468 Size: 648 1624 374 69 36 681 359 159 Cuts at: 4468 518 Size: 2042 Fragments arranged by size: 2042 1624 681 648 374 359 159 69 36 MspA1I CmG'CkG Cuts at: 1606 3033 3408 4085 5233 5478 1606 Size: 1427 375 677 1148 245 2120 Fragments arranged by size: 2120 1427 1148 677 375 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5440 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 3350 4895 1132 Size: 97 2121 1545 2229 Fragments arranged by size: 2229 2121 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5992 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PmlI CAC'GTG Cuts at: 2482 2926 2482 Size: 444 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2227 2227 Size: 5992 PshAI GACnn'nnGTC Cuts at: 1613 2095 1613 Size: 482 5510 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PstI C_TGCA'G Cuts at: 830 2082 3344 830 Size: 1252 1262 3478 Fragments arranged by size: 3478 1262 1252 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 3033 3033 Size: 5992 RsrII CG'GwC_CG Cuts at: 2652 2652 Size: 5992 SacI G_AGCT'C Cuts at: 2454 2454 Size: 5992 SalI G'TCGA_C Cuts at: 1936 2305 2938 3334 1936 Size: 369 633 396 4594 Fragments arranged by size: 4594 633 396 369 SanDI GG'GwC_CC Cuts at: 2227 2227 Size: 5992 SapI GCTCTTCn'nnn_ Cuts at: 2411 2411 Size: 5992 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SfcI C'TryA_G Cuts at: 826 1045 2063 2078 2559 3340 4475 5156 5347 Size: 219 1018 15 481 781 1135 681 191 Cuts at: 5347 826 Size: 1471 Fragments arranged by size: 1471 1135 1018 781 681 481 219 191 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 3319 1872 Size: 1447 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2855 3149 3245 4096 4997 5259 Size: 197 926 912 294 96 851 901 262 Cuts at: 5259 820 Size: 1553 Fragments arranged by size: 1553 926 912 901 851 294 262 197 96 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 3176 1517 Size: 1659 4333 StyI C'CwwG_G Cuts at: 513 1065 2423 2616 2799 2868 3048 3093 3431 Size: 552 1358 193 183 69 180 45 338 Cuts at: 3431 513 Size: 3074 Fragments arranged by size: 3074 1358 552 338 193 183 180 69 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2014 3061 3273 4019 4036 4189 4374 Size: 1255 434 1047 212 746 17 153 185 Cuts at: 4374 4819 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1047 746 445 434 212 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 2280 4238 Size: 274 80 33 51 1443 288 15 1958 Cuts at: 4238 5950 96 Size: 1712 138 Fragments arranged by size: 1958 1712 1443 288 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5952 5984 5952 Size: 32 5960 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2082 2575 2082 Size: 493 5499 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 3317 1870 Size: 1447 4545 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI SacII SexAI SfiI SfoI SrfI SwaI