(Linear) (Six-base) MAPSORT of: KIEE2077.seq Check: 5569 from: 1 to: 2235 KIEE2077.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AatII G_ACGT'C Cuts at: 0 1289 2235 Size: 1289 946 AccI GT'mk_AC Cuts at: 0 1429 2235 Size: 1429 806 AclI AA'CG_TT Cuts at: 0 1323 2235 Size: 1323 912 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 448 1216 1379 1780 2235 Size: 352 96 768 163 401 455 Fragments arranged by size: 768 455 401 352 163 96 AfeI AGC'GCT Cuts at: 0 1449 2235 Size: 1449 786 AlwNI CAG_nnn'CTG Cuts at: 0 942 2235 Size: 942 1293 ApoI r'AATT_y Cuts at: 0 589 614 672 740 1045 1948 2235 Size: 589 25 58 68 305 903 287 Fragments arranged by size: 903 589 305 287 68 58 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 527 1241 2235 Size: 527 714 994 Fragments arranged by size: 994 714 527 BclI T'GATC_A Cuts at: 0 1080 1370 2235 Size: 1080 290 865 Fragments arranged by size: 1080 865 290 BmrI ACTGGGnnnn_n' Cuts at: 0 279 324 2132 2235 Size: 279 45 1808 103 Fragments arranged by size: 1808 279 103 45 BmtI G_CTAG'C Cuts at: 0 427 2235 Size: 427 1808 BsaHI Gr'CG_yC Cuts at: 0 1286 2206 2235 Size: 1286 920 29 Fragments arranged by size: 1286 920 29 BsaWI w'CCGG_w Cuts at: 0 2024 2235 Size: 2024 211 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1548 1578 2235 Size: 1548 30 657 Fragments arranged by size: 1548 657 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 311 2030 2235 Size: 311 1719 205 Fragments arranged by size: 1719 311 205 BsmI GAATG_Cn' Cuts at: 0 488 1316 2235 Size: 488 828 919 Fragments arranged by size: 919 828 488 BspHI T'CATG_A Cuts at: 0 789 2060 2235 Size: 789 1271 175 Fragments arranged by size: 1271 789 175 BsrGI T'GTAC_A Cuts at: 0 1608 1678 2235 Size: 1608 70 557 Fragments arranged by size: 1608 557 70 BssSI C'ACGA_G Cuts at: 0 1245 2235 Size: 1245 990 BstXI CCAn_nnnn'nTGG Cuts at: 0 1350 2235 Size: 1350 885 BstZ17I GTA'TAC Cuts at: 0 1430 2235 Size: 1430 805 BtgI C'CryG_G Cuts at: 0 68 543 1724 2235 Size: 68 475 1181 511 Fragments arranged by size: 1181 511 475 68 DraI TTT'AAA Cuts at: 0 1953 2235 Size: 1953 282 DrdI GACnn_nn'nnGTC Cuts at: 0 1285 2235 Size: 1285 950 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 448 1216 1379 1780 2235 Size: 352 96 768 163 401 455 Fragments arranged by size: 768 455 401 352 163 96 EcoNI CCTnn'n_nnAGG Cuts at: 0 991 2235 Size: 991 1244 EcoO109I rG'GnC_Cy Cuts at: 0 966 1110 2235 Size: 966 144 1125 Fragments arranged by size: 1125 966 144 EcoRV GAT'ATC Cuts at: 0 123 2235 Size: 123 2112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 382 1305 2235 Size: 382 923 930 Fragments arranged by size: 930 923 382 HaeII r_GCGC'y Cuts at: 0 1451 2235 Size: 1451 784 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 100 132 1888 1920 2235 Size: 100 32 1756 32 315 Fragments arranged by size: 1756 315 100 32 32 HincII GTy'rAC Cuts at: 0 799 2235 Size: 799 1436 HindIII A'AGCT_T Cuts at: 0 665 2235 Size: 665 1570 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 59 1082 2235 Size: 59 1023 1153 Fragments arranged by size: 1153 1023 59 MslI CAynn'nnrTG Cuts at: 0 956 1258 1348 1643 1810 2235 Size: 956 302 90 295 167 425 Fragments arranged by size: 956 425 302 295 167 90 NcoI C'CATG_G Cuts at: 0 543 1724 2235 Size: 543 1181 511 Fragments arranged by size: 1181 543 511 NdeI CA'TA_TG Cuts at: 0 929 1716 2235 Size: 929 787 519 Fragments arranged by size: 929 787 519 NheI G'CTAG_C Cuts at: 0 423 2235 Size: 423 1812 NspI r_CATG'y Cuts at: 0 838 1005 2235 Size: 838 167 1230 Fragments arranged by size: 1230 838 167 PsiI TTA'TAA Cuts at: 0 1787 2235 Size: 1787 448 ScaI AGT'ACT Cuts at: 0 248 1180 2235 Size: 248 932 1055 Fragments arranged by size: 1055 932 248 SfcI C'TryA_G Cuts at: 0 1534 2048 2235 Size: 1534 514 187 Fragments arranged by size: 1534 514 187 StyI C'CwwG_G Cuts at: 0 156 543 1724 2235 Size: 156 387 1181 511 Fragments arranged by size: 1181 511 387 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 343 2235 Size: 343 1892 TatI w'GTAC_w Cuts at: 0 246 1178 1608 1678 2235 Size: 246 932 430 70 557 Fragments arranged by size: 932 557 430 246 70 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1642 2235 Size: 1642 593 XmnI GAAnn'nnTTC Cuts at: 0 461 589 713 740 2235 Size: 461 128 124 27 1495 Fragments arranged by size: 1495 461 128 124 27 ZraI GAC'GTC Cuts at: 0 1287 2235 Size: 1287 948 Enzymes that do cut: AatII AccI AclI AcuI AfeI AlwNI ApoI BbsI BclI BmrI BmtI BsaHI BsaWI BsaXI BsgI BsmI BspHI BsrGI BssSI BstXI BstZ17I BtgI DraI DrdI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII MmeI MslI NcoI NdeI NheI NspI PsiI ScaI SfcI StyI TaqII TatI XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI ClaI CspCI DraIII EaeI EagI EarI EciI EcoICRI EcoRI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NgoMIV NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI