################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaI BsiWI BsmBI Bsp1407I BspDI BspLU11I BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco81I EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HpaI Ksp632I KspI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 110 GGTACC G^GTACC AccIII 180 TCCGGA T^CCGGA AcyI 1027 GGCGCC G[GA]^CG[CT]C Aor51HI 911 AGCGCT AGC^GCT Asp718I 110 GGTACC G^GTACC AspHI 31 GTGCTC G[AT]GC[AT]^C BalI 1880 TGGCCA TGG^CCA BanII 1723 GGGCTC G[GA]GC[CT]^C BbeI 1027 GGCGCC GGCGC^C BciVI 974 GGATAC GTATCC(6/5) BsaAI 256 TACGTG [CT]AC^GT[GA] BsaBI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1027 GGCGCC G[GA]^CG[CT]C BsaWI 180 TCCGGA [AT]^CCGG[AT] BseAI 180 TCCGGA T^CCGGA BsgI 392 GTGCAG GTGCAG(16/14) BsiHKAI 31 GTGCTC G[AT]GC[AT]^C BspEI 180 TCCGGA T^CCGGA BspHI 1532 TCATGA T^CATGA BsrBI 16 CCGCTC CCGCTC(-3/-3) Bst1107I 1261 GTATAC GTA^TAC BstEII 1392 GGTAACC G^GT[ACGT]ACC BstPI 1392 GGTAACC G^GT[ACGT]ACC BstXI 1871 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1688 GGATCT [GA]^GATC[CT] BstZ17I 1261 GTATAC GTA^TAC DraII 429 AGGTCCT [GA]G^G[ACGT]CC[CT] EagI 53 CGGCCG C^GGCCG EclXI 53 CGGCCG C^GGCCG Eco47III 911 AGCGCT AGC^GCT Eco52I 53 CGGCCG C^GGCCG EcoNI 1464 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 429 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1392 GGTAACC G^GT[ACGT]ACC Hin1I 1027 GGCGCC G[GA]^CG[CT]C HindIII 570 AAGCTT A^AGCTT KasI 1027 GGCGCC G^GCGCC KpnI 110 GGTACC GGTAC^C MamI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1688 GGATCT [GA]^GATC[CT] MluNI 1880 TGGCCA TGG^CCA MroI 180 TCCGGA T^CCGGA MscI 1880 TGGCCA TGG^CCA MspA1I 1766 CAGCTG C[AC]G^C[GT]G NaeI 55 GCCGGC GCC^GGC NarI 1027 GGCGCC GG^CGCC NcoI 731 CCATGG C^CATGG NgoMIV 55 GCCGGC G^CCGGC PpuMI 429 AGGTCCT [GA]G^G[AT]CC[CT] PstI 905 CTGCAG CTGCA^G PvuII 1766 CAGCTG CAG^CTG RcaI 1532 TCATGA T^CATGA SexAI 696 ACCAGGT A^CC[AT]GGT SfoI 1027 GGCGCC GGC^GCC SmaI 106 CCCGGG CCC^GGG SspI 1055 AATATT AAT^ATT Tsp45I 1006 GTCAC ^GT[GC]AC XhoII 1688 GGATCT [GA]^GATC[CT] XmaCI 106 CCCGGG C^CCGGG XmaI 106 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 982 GTAGAC GT^[AC][GT]AC 1261 GTATAC AccII 753 CGCG CG^CG 1676 CGCG AfaI 111 GTAC GT^AC 1554 GTAC AlwNI 237 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1362 CAGAAACTG AvaI 106 CCCGGG C^[CT]CG[GA]G 1696 CCCGAG BclI 203 TGATCA T^GATCA 1583 TGATCA BfaI 461 CTAG C^TAG 631 CTAG BpmI 599 CTCCAG CTGGAG(16/14) 580 CTGGAG BsiEI 53 CGGCCG CG[GA][CT]^CG 624 CGACCG BsmI 514 GAATGC GAATGC(1/-1) 722 GCATTC BsoBI 106 CCCGGG C^[CT]CG[GA]G 1696 CCCGAG BsrFI 55 GCCGGC [GA]^CCGG[CT] 626 ACCGGC BstUI 753 CGCG CG^CG 1676 CGCG Cfr10I 55 GCCGGC [GA]^CCGG[CT] 626 ACCGGC DraIII 386 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 458 CACCTAGTG DsaI 731 CCATGG C^C[GA][CT]GG 1895 CCACGG EaeI 53 CGGCCG [CT]^GGCC[GA] 1880 TGGCCA EcoT14I 731 CCATGG C^C[AT][AT]GG 1353 CCAAGG FauI 917 CCCGC CCCGC(4/6) 1423 CCCGC FbaI 203 TGATCA T^GATCA 1583 TGATCA MaeI 461 CTAG C^TAG 631 CTAG MvnI 753 CGCG CG^CG 1676 CGCG RsaI 111 GTAC GT^AC 1554 GTAC SfaNI 1371 GATGC GCATC(5/9) 1490 GCATC StyI 731 CCATGG C^C[AT][AT]GG 1353 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BanI BbsI BbvI BcnI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI TaqI TfiI TseI Tsp509I TspRI TthHB8I XcmI