################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BciVI BcnI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT14I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 448 GTATAC GT^[AC][GT]AC AflII 49 CTTAAG C^TTAAG Alw44I 1116 GTGCAC G^TGCAC AlwI 1273 GATCC GGATC(4/5) AocI 31 CCTGAGG CC^T[ACGT]AGG ApaI 1157 GGGCCC GGGCC^C ApaLI 1116 GTGCAC G^TGCAC AspHI 1116 GTGCAC G[AT]GC[AT]^C AvaII 304 GGACC G^G[AT]CC BbrPI 1113 CACGTG CAC^GTG BbsI 1333 GAAGAC GAAGAC(2/6) BclI 771 TGATCA T^GATCA BfaI 715 CTAG C^TAG BfrI 49 CTTAAG C^TTAAG BpuAI 1333 GAAGAC GAAGAC(2/6) BsaAI 1113 CACGTG [CT]AC^GT[GA] BsaWI 1293 ACCGGA [AT]^CCGG[AT] BsgI 725 GTGCAG GTGCAG(16/14) BsiHKAI 1116 GTGCAC G[AT]GC[AT]^C BsmFI 987 GGGAC GGGAC(10/14) BsmI 831 GAATGC GAATGC(1/-1) Bst1107I 448 GTATAC GTA^TAC BstZ17I 448 GTATAC GTA^TAC Bsu36I 31 CCTGAGG CC^T[ACGT]AGG DraII 1157 GGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 1130 TGGCCG [CT]^GGCC[GA] Eco81I 31 CCTGAGG CC^T[ACGT]AGG EcoO109I 1157 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 18 GATATC GAT^ATC EcoT22I 766 ATGCAT ATGCA^T FbaI 771 TGATCA T^GATCA HapII 1294 CCGG C^CGG HgaI 1344 GCGTC GACGC(5/10) HpaII 1294 CCGG C^CGG MaeI 715 CTAG C^TAG MaeII 1114 ACGT A^CGT MspI 1294 CCGG C^CGG NsiI 766 ATGCAT ATGCA^T PmaCI 1113 CACGTG CAC^GTG PmlI 1113 CACGTG CAC^GTG SmlI 49 CTTAAG C^T[CT][GA]AG SspI 316 AATATT AAT^ATT XbaI 714 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 195 ACATGT A^C[GA][CT]GT 1337 ACATGT AlwNI 728 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 1078 CAGCTGCTG BsaI 27 GAGACC GGTCTC(1/5) 993 GAGACC BseRI 377 GAGGAG GAGGAG(10/8) 1211 GAGGAG BsmAI 27 GAGAC GTCTC(1/5) 993 GAGAC BspLU11I 195 ACATGT A^CATGT 1337 ACATGT BsrDI 753 CATTGC GCAATG(2/0) 362 GCAATG DpnII 772 GATC ^GATC 1273 GATC EarI 1060 CTCTTC CTCTTC(1/4) 1328 GAAGAG EcoRI 852 GAATTC G^AATTC 956 GAATTC Ksp632I 1060 CTCTTC CTCTTC(1/4) 1328 GAAGAG MboI 772 GATC ^GATC 1273 GATC MwoI 1044 GCCATTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1080 GCTGCTGAAGC NdeII 772 GATC ^GATC 1273 GATC NspI 195 ACATGT [GA]CATG^[CT] 1337 ACATGT PleI 1309 GACTC GAGTC(4/5) 1360 GACTC PstI 1067 CTGCAG CTGCA^G 1261 CTGCAG PvuII 1078 CAGCTG CAG^CTG 1258 CAGCTG Sau3AI 772 GATC ^GATC 1273 GATC TfiI 711 GATTC G^A[AT]TC 943 GAATC Tsp45I 79 GTGAC ^GT[GC]AC 138 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BanII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboII MnlI MseI MslI MspA1I MvaI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp509I TspRI