(Circular) (Six-base) MAPSORT of: pFN21AE6787.seq Check: 8387 from: 1 to: 6778 pFN21AE6787 6778 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4588 278 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4121 1937 Size: 2184 4594 Acc65I G'GTAC_C Cuts at: 4099 4099 Size: 6778 AclI AA'CG_TT Cuts at: 1563 4905 5278 1563 Size: 3342 373 3063 Fragments arranged by size: 3342 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4841 6225 6493 1632 Size: 3209 1384 268 1917 Fragments arranged by size: 3209 1917 1384 268 AfeI AGC'GCT Cuts at: 3162 3162 Size: 6778 AflII C'TTAA_G Cuts at: 820 1017 3781 820 Size: 197 2764 3817 Fragments arranged by size: 3817 2764 197 AflIII A'CryG_T Cuts at: 1203 5677 6654 1203 Size: 4474 977 1327 Fragments arranged by size: 4474 1327 977 AgeI A'CCGG_T Cuts at: 5609 5609 Size: 6778 AhdI GACnn_n'nnGTC Cuts at: 5507 5507 Size: 6778 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6778 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3678 1184 Size: 2494 4284 AlwNI CAG_nnn'CTG Cuts at: 3433 3914 4521 6093 3433 Size: 481 607 1572 4118 Fragments arranged by size: 4118 1572 607 481 ApaLI G'TGCA_C Cuts at: 4835 5991 4835 Size: 1156 5622 ApoI r'AATT_y Cuts at: 1493 2305 2426 3305 3820 4087 4313 4465 6574 Size: 812 121 879 515 267 226 152 2109 Cuts at: 6574 1493 Size: 1697 Fragments arranged by size: 2109 1697 879 812 515 267 226 152 121 AseI AT'TA_AT Cuts at: 160 1032 3356 5332 160 Size: 872 2324 1976 1606 Fragments arranged by size: 2324 1976 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 AvaI C'yCGr_G Cuts at: 1870 1943 4103 1870 Size: 73 2160 4545 Fragments arranged by size: 4545 2160 73 AvrII C'CTAG_G Cuts at: 4024 4024 Size: 6778 BamHI G'GATC_C Cuts at: 4108 4475 4108 Size: 367 6411 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2223 4099 5554 618 Size: 325 217 627 251 185 1876 1455 1842 Fragments arranged by size: 1876 1842 1455 627 325 251 217 185 BanII G_rGCy'C Cuts at: 1391 3146 4097 1391 Size: 1755 951 4072 Fragments arranged by size: 4072 1755 951 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3809 928 Size: 971 1910 3897 Fragments arranged by size: 3897 1910 971 BbvCI CC'TCA_GC Cuts at: 2092 3038 2092 Size: 946 5832 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4969 5003 1903 Size: 34 3032 34 3678 Fragments arranged by size: 3678 3032 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2041 4670 5880 2041 Size: 2629 1210 2939 Fragments arranged by size: 2939 2629 1210 BclI T'GATC_A Cuts at: 1548 2637 2857 1548 Size: 1089 220 5469 Fragments arranged by size: 5469 1089 220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5389 136 Size: 107 122 71 631 605 3717 1525 Fragments arranged by size: 3717 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2385 6773 2385 Size: 4388 2390 BlpI GC'TnA_GC Cuts at: 4195 4195 Size: 6778 Bme1580I G_kGCm'C Cuts at: 1790 2228 3453 4839 5995 1790 Size: 438 1225 1386 1156 2573 Fragments arranged by size: 2573 1386 1225 1156 438 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6778 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2229 3149 4509 Size: 416 53 83 399 24 812 920 1360 Cuts at: 4509 5467 442 Size: 958 1753 Fragments arranged by size: 1753 1360 958 920 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6778 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2364 2364 Size: 6778 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2319 2639 3116 5438 1239 Size: 1080 320 477 2322 2579 Fragments arranged by size: 2579 2322 1080 477 320 Bpu10I CC'TnA_GC Cuts at: 2092 3038 2092 Size: 946 5832 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2170 4903 5768 6066 2170 Size: 2733 865 298 2882 Fragments arranged by size: 2882 2733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5441 882 Size: 617 3942 2219 Fragments arranged by size: 3942 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3454 4569 493 Size: 711 2250 1115 2702 Fragments arranged by size: 2702 2250 1115 711 BsaBI GATnn'nnATC Cuts at: 2259 3731 6772 2259 Size: 1472 3041 2265 Fragments arranged by size: 3041 2265 1472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4585 4967 5602 275 Size: 53 83 186 936 3052 382 635 1451 Fragments arranged by size: 3052 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2013 5211 5609 5883 6030 6441 2013 Size: 3198 398 274 147 411 2350 Fragments arranged by size: 3198 2350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6553 Fragments arranged by size: 6553 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2248 2759 3026 3200 1186 Size: 1062 511 267 174 4764 Fragments arranged by size: 4764 1062 511 267 174 BseYI C'CCAG_C Cuts at: 3151 3917 5981 6477 3151 Size: 766 2064 496 3452 Fragments arranged by size: 3452 2064 766 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6205 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4228 4989 6017 1540 Size: 275 186 2227 761 1028 2301 Fragments arranged by size: 2301 2227 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3780 4839 4924 5995 3780 Size: 1059 85 1071 4563 Fragments arranged by size: 4563 1071 1059 85 BsiWI C'GTAC_G Cuts at: 2732 2732 Size: 6778 BsmI GAATG_Cn' Cuts at: 2250 4289 4382 2250 Size: 2039 93 4646 Fragments arranged by size: 4646 2039 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 3146 3453 3780 4097 4839 4924 Size: 399 438 918 307 327 317 742 85 Cuts at: 4924 5995 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 918 742 438 399 327 317 307 85 BspEI T'CCGG_A Cuts at: 2013 6441 2013 Size: 4428 2350 BspHI T'CATG_A Cuts at: 3630 3742 4667 6397 3630 Size: 112 925 1730 4011 Fragments arranged by size: 4011 1730 925 112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2148 2939 4133 844 Size: 1304 791 1194 3489 Fragments arranged by size: 3489 1304 1194 791 BsrBI CCG'CTC Cuts at: 1394 1823 4665 1394 Size: 429 2842 3507 Fragments arranged by size: 3507 2842 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5273 5447 65 Size: 1183 4025 174 1396 Fragments arranged by size: 4025 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2131 5422 5609 1700 Size: 110 321 3291 187 2869 Fragments arranged by size: 3291 2869 321 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6778 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6778 BssSI C'ACGA_G Cuts at: 4838 5850 4838 Size: 1012 5766 BstAPI GCAn_nnn'nTGC Cuts at: 794 2265 794 Size: 1471 5307 BstBI TT'CG_AA Cuts at: 5582 5582 Size: 6778 BstEII G'GTnAC_C Cuts at: 1184 2162 1184 Size: 978 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2919 2919 Size: 6778 BstYI r'GATC_y Cuts at: 1972 2385 3900 4108 4475 4860 4877 6329 6415 Size: 413 1515 208 367 385 17 1452 86 Cuts at: 6415 6427 6773 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1515 1452 413 385 367 346 208 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2453 3270 2453 Size: 817 5961 BtgI C'CryG_G Cuts at: 513 1065 2113 2228 2886 3370 513 Size: 552 1048 115 658 484 3921 Fragments arranged by size: 3921 1048 658 552 484 115 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6039 Fragments arranged by size: 6039 683 56 BtsI GCAGTG_nn' Cuts at: 2599 3912 4294 5088 5108 2599 Size: 1313 382 794 20 4269 Fragments arranged by size: 4269 1313 794 382 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3709 3744 529 Size: 35 3145 35 3563 Fragments arranged by size: 3563 3145 35 35 DraI TTT'AAA Cuts at: 4082 4082 Size: 6778 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5785 809 Size: 507 4469 1802 Fragments arranged by size: 4469 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3225 4225 5114 Size: 54 1425 50 275 18 1395 1000 889 Cuts at: 5114 6743 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1395 1000 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4225 1537 Size: 275 2413 4090 Fragments arranged by size: 4090 2413 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 2376 2884 4708 1353 Size: 1002 21 508 1824 3423 Fragments arranged by size: 3423 1824 1002 508 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2735 5365 5739 5885 387 Size: 1064 138 1146 2630 374 146 1280 Fragments arranged by size: 2630 1280 1146 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2319 2639 3116 4841 5438 6225 6493 Size: 393 687 320 477 1725 597 787 268 Cuts at: 6493 1239 Size: 1524 Fragments arranged by size: 1725 1524 787 687 597 477 393 320 268 EcoNI CCTnn'n_nnAGG Cuts at: 2144 2456 2144 Size: 312 6466 EcoO109I rG'GnC_Cy Cuts at: 2974 3430 2974 Size: 456 6322 EcoRI G'AATT_C Cuts at: 4087 4465 4087 Size: 378 6400 EcoRV GAT'ATC Cuts at: 2503 2503 Size: 6778 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2336 3016 2336 Size: 680 6098 FspI TGC'GCA Cuts at: 5284 5284 Size: 6778 HaeII r_GCGC'y Cuts at: 1678 3164 5925 1678 Size: 1486 2761 2531 Fragments arranged by size: 2761 2531 1486 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 5419 5451 5493 Size: 32 135 32 503 32 3312 32 42 Cuts at: 5493 5525 1373 Size: 32 2626 Fragments arranged by size: 3312 2626 503 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4122 4368 669 Size: 988 281 2184 246 3079 Fragments arranged by size: 3079 2184 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6778 HpaI GTT'AAC Cuts at: 4368 4368 Size: 6778 KpnI G_GTAC'C Cuts at: 4103 4103 Size: 6778 MfeI C'AATT_G Cuts at: 4377 4377 Size: 6778 MluI A'CGCG_T Cuts at: 6654 6654 Size: 6778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2190 3618 5892 6076 1305 Size: 885 1428 2274 184 2007 Fragments arranged by size: 2274 2007 1428 885 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4956 Fragments arranged by size: 4956 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3180 3318 3815 4736 5095 5254 518 Size: 648 2014 138 497 921 359 159 2042 Fragments arranged by size: 2042 2014 921 648 497 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2950 3042 3211 3563 3878 4194 4871 6019 Size: 1344 92 169 352 315 316 677 1148 Cuts at: 6019 6264 1606 Size: 245 2120 Fragments arranged by size: 2120 1344 1148 677 352 316 315 245 169 92 NcoI C'CATG_G Cuts at: 513 1065 2886 3370 513 Size: 552 1821 484 3921 Fragments arranged by size: 3921 1821 552 484 NdeI CA'TA_TG Cuts at: 387 387 Size: 6778 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6778 NotI GC'GGCC_GC Cuts at: 4225 4225 Size: 6778 NspI r_CATG'y Cuts at: 1132 1229 4136 5681 1132 Size: 97 2907 1545 2229 Fragments arranged by size: 2907 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6778 PciI A'CATG_T Cuts at: 5677 5677 Size: 6778 PflMI CCAn_nnn'nTGG Cuts at: 1108 2551 3308 1108 Size: 1443 757 4578 Fragments arranged by size: 4578 1443 757 PmeI GTTT'AAAC Cuts at: 4082 4082 Size: 6778 PmlI CAC'GTG Cuts at: 3454 3454 Size: 6778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4847 4879 6360 6392 4847 Size: 32 1481 32 5233 Fragments arranged by size: 5233 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6778 PsiI TTA'TAA Cuts at: 3827 4348 3827 Size: 521 6257 PstI C_TGCA'G Cuts at: 830 2571 4130 830 Size: 1741 1559 3478 Fragments arranged by size: 3478 1741 1559 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6778 PvuII CAG'CTG Cuts at: 2950 3042 3211 3563 3878 2950 Size: 92 169 352 315 5850 Fragments arranged by size: 5850 352 315 169 92 SalI G'TCGA_C Cuts at: 1936 4120 1936 Size: 2184 4594 SbfI CC_TGCA'GG Cuts at: 4130 4130 Size: 6778 ScaI AGT'ACT Cuts at: 2525 2794 5026 6738 2525 Size: 269 2232 1712 2565 Fragments arranged by size: 2565 2232 1712 269 SfcI C'TryA_G Cuts at: 826 1045 2144 2567 2966 3109 3193 3573 4126 Size: 219 1099 423 399 143 84 380 553 Cuts at: 4126 5261 5942 6133 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1099 681 553 423 399 380 219 191 143 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6778 SmaI CCC'GGG Cuts at: 1872 4105 1872 Size: 2233 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2185 3781 4882 5783 6045 820 Size: 197 926 242 1596 1101 901 262 1553 Fragments arranged by size: 1596 1553 1101 926 901 262 242 197 SnaBI TAC'GTA Cuts at: 493 4569 493 Size: 4076 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6778 SphI G_CATG'C Cuts at: 1132 4136 1132 Size: 3004 3774 SspI AAT'ATT Cuts at: 5 52 4069 4702 5 Size: 47 4017 633 2081 Fragments arranged by size: 4017 2081 633 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6778 StyI C'CwwG_G Cuts at: 513 1065 2886 3060 3370 3939 4024 4217 513 Size: 552 1821 174 310 569 85 193 3074 Fragments arranged by size: 3074 1821 569 552 310 193 174 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2184 2211 4805 4822 4975 5160 Size: 1255 446 158 27 2594 17 153 185 Cuts at: 5160 5605 325 Size: 445 1498 Fragments arranged by size: 2594 1498 1255 446 445 185 158 153 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 2792 5024 Size: 274 80 33 51 1443 546 269 2232 Cuts at: 5024 6736 96 Size: 1712 138 Fragments arranged by size: 2232 1712 1443 546 274 269 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6738 6770 6738 Size: 32 6746 Tth111I GACn'n_nGTC Cuts at: 1316 3238 1316 Size: 1922 4856 XbaI T'CTAG_A Cuts at: 4114 4114 Size: 6778 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6778 XmaI C'CCGG_G Cuts at: 1870 4103 1870 Size: 2233 4545 XmnI GAAnn'nnTTC Cuts at: 3951 4907 3951 Size: 956 5822 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4586 276 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BfrBI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI