(Circular) (Six-base) MAPSORT of: pFN21AE1972.seq Check: 3780 from: 1 to: 6325 pFN21AE1972 6325 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3441 3668 1937 Size: 1504 227 4594 Fragments arranged by size: 4594 1504 227 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 4388 5772 6040 1632 Size: 1065 1691 1384 268 1917 Fragments arranged by size: 1917 1691 1384 1065 268 AflII C'TTAA_G Cuts at: 820 1017 2408 820 Size: 197 1391 4737 Fragments arranged by size: 4737 1391 197 AflIII A'CryG_T Cuts at: 1203 5224 6201 1203 Size: 4021 977 1327 Fragments arranged by size: 4021 1327 977 AgeI A'CCGG_T Cuts at: 5156 5156 Size: 6325 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6325 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AlwNI CAG_nnn'CTG Cuts at: 2944 4068 5640 2944 Size: 1124 1572 3629 Fragments arranged by size: 3629 1572 1124 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 2238 2357 3634 3860 4012 6121 1493 Size: 745 119 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 745 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 3650 1870 Size: 73 1707 4545 Fragments arranged by size: 4545 1707 73 BamHI G'GATC_C Cuts at: 2491 3655 4022 2491 Size: 1164 367 4794 Fragments arranged by size: 4794 1164 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3239 3433 3526 3646 5101 Size: 325 217 627 1452 194 93 120 1455 Cuts at: 5101 618 Size: 1842 Fragments arranged by size: 1842 1455 1452 627 325 217 194 120 93 BanII G_rGCy'C Cuts at: 1391 3644 1391 Size: 2253 4072 BbeI G_GCGC'C Cuts at: 3437 3437 Size: 6325 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2135 2685 2849 3284 3303 928 Size: 971 236 550 164 435 19 3950 Fragments arranged by size: 3950 971 550 435 236 164 19 BbvCI CC'TCA_GC Cuts at: 2073 3561 3588 2073 Size: 1488 27 4810 Fragments arranged by size: 4810 1488 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4516 4550 1903 Size: 34 50 34 2495 34 3678 Fragments arranged by size: 3678 2495 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2607 4217 5427 2607 Size: 1610 1210 3505 Fragments arranged by size: 3505 1610 1210 BclI T'GATC_A Cuts at: 1548 2968 1548 Size: 1420 4905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2803 6320 2803 Size: 3517 2808 BlpI GC'TnA_GC Cuts at: 3742 3742 Size: 6325 Bme1580I G_kGCm'C Cuts at: 1790 4386 5542 1790 Size: 2596 1156 2573 Fragments arranged by size: 2596 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2089 2955 4056 Size: 416 53 83 399 24 672 866 1101 Cuts at: 4056 5014 442 Size: 958 1753 Fragments arranged by size: 1753 1101 958 866 672 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2724 3263 4985 1239 Size: 1485 539 1722 2579 Fragments arranged by size: 2579 1722 1485 539 Bpu10I CC'TnA_GC Cuts at: 2073 3561 3588 2073 Size: 1488 27 4810 Fragments arranged by size: 4810 1488 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 4450 5315 5613 2540 Size: 1910 865 298 3252 Fragments arranged by size: 3252 1910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4988 882 Size: 617 3489 2219 Fragments arranged by size: 3489 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4116 493 Size: 711 2912 2702 Fragments arranged by size: 2912 2702 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3434 4132 4514 5149 Size: 53 83 186 936 1901 698 382 635 Cuts at: 5149 275 Size: 1451 Fragments arranged by size: 1901 1451 936 698 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4758 5156 5430 5577 5988 4758 Size: 398 274 147 411 5095 Fragments arranged by size: 5095 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2205 2235 3293 3323 1178 Size: 30 165 30 802 30 1058 30 4180 Fragments arranged by size: 4180 1058 802 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3219 3441 1186 Size: 2033 222 4070 Fragments arranged by size: 4070 2033 222 BseYI C'CCAG_C Cuts at: 2431 2473 3349 3425 5528 6024 2431 Size: 42 876 76 2103 496 2732 Fragments arranged by size: 2732 2103 876 496 76 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5752 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3775 4536 5564 1540 Size: 275 186 1774 761 1028 2301 Fragments arranged by size: 2301 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4386 4471 5542 4386 Size: 85 1071 5169 Fragments arranged by size: 5169 1071 85 BsmI GAATG_Cn' Cuts at: 3508 3836 3929 3508 Size: 328 93 5904 Fragments arranged by size: 5904 328 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3644 4386 4471 5542 1391 Size: 399 1854 742 85 1071 2174 Fragments arranged by size: 2174 1854 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3680 844 Size: 2836 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4212 1394 Size: 429 2389 3507 Fragments arranged by size: 3507 2389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2400 3012 4820 4994 65 Size: 1183 1152 612 1808 174 1396 Fragments arranged by size: 1808 1396 1183 1152 612 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4969 5156 1700 Size: 110 3159 187 2869 Fragments arranged by size: 3159 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstYI r'GATC_y Cuts at: 1972 2176 2491 2803 3136 3655 4022 4407 4424 Size: 204 315 312 333 519 367 385 17 Cuts at: 4424 5876 5962 5974 6320 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 519 385 367 346 333 315 312 204 86 17 12 BstZ17I GTA'TAC Cuts at: 3442 3442 Size: 6325 Bsu36I CC'TnA_GG Cuts at: 2133 2133 Size: 6325 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5773 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 2808 3333 3841 4635 4655 2808 Size: 525 508 794 20 4478 Fragments arranged by size: 4478 794 525 508 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 2698 3629 2698 Size: 931 5394 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290 Size: 54 1425 50 275 18 1942 889 1629 Cuts at: 6290 8 Size: 43 Fragments arranged by size: 1942 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2155 2211 4255 1353 Size: 802 56 2044 3423 Fragments arranged by size: 3423 2044 802 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2697 2724 3263 4388 4985 5772 6040 Size: 393 1065 27 539 1125 597 787 268 Cuts at: 6040 1239 Size: 1524 Fragments arranged by size: 1524 1125 1065 787 597 539 393 268 27 EcoO109I rG'GnC_Cy Cuts at: 2083 3023 3103 3224 3509 2083 Size: 940 80 121 285 4899 Fragments arranged by size: 4899 940 285 121 80 EcoRI G'AATT_C Cuts at: 3634 4012 3634 Size: 378 5947 EcoRV GAT'ATC Cuts at: 2098 2098 Size: 6325 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 3437 5472 1678 Size: 1759 2035 2531 Fragments arranged by size: 2531 2035 1759 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2788 2820 3010 3042 3274 Size: 32 135 32 1216 32 190 32 232 Cuts at: 3274 3293 3306 3325 4966 4998 5040 5072 1373 Size: 19 13 19 1641 32 42 32 2626 Fragments arranged by size: 2626 1641 1216 232 190 135 42 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2577 3669 3915 669 Size: 988 281 639 1092 246 3079 Fragments arranged by size: 3079 1092 988 639 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KasI G'GCGC_C Cuts at: 3433 3433 Size: 6325 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5439 5623 1305 Size: 4134 184 2007 Fragments arranged by size: 4134 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2631 4283 4642 4801 518 Size: 648 1465 1652 359 159 2042 Fragments arranged by size: 2042 1652 1465 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3307 3741 4418 5566 5811 1606 Size: 1701 434 677 1148 245 2120 Fragments arranged by size: 2120 1701 1148 677 434 245 NarI GG'CG_CC Cuts at: 3434 3434 Size: 6325 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5773 NdeI CA'TA_TG Cuts at: 387 387 Size: 6325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 2220 3683 5228 1132 Size: 97 991 1463 1545 2229 Fragments arranged by size: 2229 1545 1463 991 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6325 PciI A'CATG_T Cuts at: 5224 5224 Size: 6325 PflMI CCAn_nnn'nTGG Cuts at: 1108 2438 3328 1108 Size: 1330 890 4105 Fragments arranged by size: 4105 1330 890 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3023 3509 3023 Size: 486 5839 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PstI C_TGCA'G Cuts at: 830 2455 2803 3677 830 Size: 1625 348 874 3478 Fragments arranged by size: 3478 1625 874 348 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 PvuII CAG'CTG Cuts at: 3307 3307 Size: 6325 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SexAI A'CCwGG_T Cuts at: 3246 3246 Size: 6325 SfcI C'TryA_G Cuts at: 826 1045 2451 2799 2936 3286 3673 4808 5489 Size: 219 1406 348 137 350 387 1135 681 Cuts at: 5489 5680 826 Size: 191 1471 Fragments arranged by size: 1471 1406 1135 681 387 350 348 219 191 137 SfoI GGC'GCC Cuts at: 3435 3435 Size: 6325 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2555 4429 5330 5592 820 Size: 197 926 465 147 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 465 262 197 147 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6325 StyI C'CwwG_G Cuts at: 513 1065 2487 3764 513 Size: 552 1422 1277 3074 Fragments arranged by size: 3074 1422 1277 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2136 4352 4369 4522 4707 5152 325 Size: 1255 556 2216 17 153 185 445 1498 Fragments arranged by size: 2216 1498 1255 556 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2951 4571 6283 Size: 274 80 33 51 1443 974 1620 1712 Cuts at: 6283 96 Size: 138 Fragments arranged by size: 1712 1620 1443 974 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2581 2613 6285 6317 2581 Size: 32 3672 32 2589 Fragments arranged by size: 3672 2589 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 3064 4454 3064 Size: 1390 4935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI