(Linear) (Six-base) MAPSORT of: KIEE1972.seq Check: 7543 from: 1 to: 1620 KIEE1972.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AccI GT'mk_AC Cuts at: 0 1436 1620 Size: 1436 184 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 692 1620 Size: 692 928 AflII C'TTAA_G Cuts at: 0 403 1620 Size: 403 1217 AlwNI CAG_nnn'CTG Cuts at: 0 939 1620 Size: 939 681 ApoI r'AATT_y Cuts at: 0 233 352 1620 Size: 233 119 1268 Fragments arranged by size: 1268 233 119 BamHI G'GATC_C Cuts at: 0 486 1620 Size: 486 1134 BanI G'GyrC_C Cuts at: 0 1234 1428 1521 1620 Size: 1234 194 93 99 Fragments arranged by size: 1234 194 99 93 BbeI G_GCGC'C Cuts at: 0 1432 1620 Size: 1432 188 BbsI GAAGACnn'nnnn_ Cuts at: 0 130 680 844 1279 1298 1620 Size: 130 550 164 435 19 322 Fragments arranged by size: 550 435 322 164 130 19 BbvCI CC'TCA_GC Cuts at: 0 68 1556 1583 1620 Size: 68 1488 27 37 Fragments arranged by size: 1488 68 37 27 BciVI GTATCCnnnnn_n' Cuts at: 0 602 1620 Size: 602 1018 BclI T'GATC_A Cuts at: 0 963 1620 Size: 963 657 BglII A'GATC_T Cuts at: 0 798 1620 Size: 798 822 BmrI ACTGGGnnnn_n' Cuts at: 0 84 950 1620 Size: 84 866 670 Fragments arranged by size: 866 670 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 719 1258 1620 Size: 719 539 362 Fragments arranged by size: 719 539 362 Bpu10I CC'TnA_GC Cuts at: 0 68 1556 1583 1620 Size: 68 1488 27 37 Fragments arranged by size: 1488 68 37 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 1620 Size: 535 1085 BsaHI Gr'CG_yC Cuts at: 0 1429 1620 Size: 1429 191 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 200 230 1288 1318 1620 Size: 200 30 1058 30 302 Fragments arranged by size: 1058 302 200 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1214 1436 1620 Size: 1214 222 184 Fragments arranged by size: 1214 222 184 BseYI C'CCAG_C Cuts at: 0 426 468 1344 1420 1620 Size: 426 42 876 76 200 Fragments arranged by size: 876 426 200 76 42 BsmI GAATG_Cn' Cuts at: 0 1503 1620 Size: 1503 117 BsrDI GCAATG_nn' Cuts at: 0 395 1007 1620 Size: 395 612 613 Fragments arranged by size: 613 612 395 BstYI r'GATC_y Cuts at: 0 171 486 798 1131 1620 Size: 171 315 312 333 489 Fragments arranged by size: 489 333 315 312 171 BstZ17I GTA'TAC Cuts at: 0 1437 1620 Size: 1437 183 Bsu36I CC'TnA_GG Cuts at: 0 128 1620 Size: 128 1492 BtsI GCAGTG_nn' Cuts at: 0 803 1328 1620 Size: 803 525 292 Fragments arranged by size: 803 525 292 DraI TTT'AAA Cuts at: 0 693 1620 Size: 693 927 EarI CTCTTCn'nnn_ Cuts at: 0 150 206 1620 Size: 150 56 1414 Fragments arranged by size: 1414 150 56 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 692 719 1258 1620 Size: 692 27 539 362 Fragments arranged by size: 692 539 362 27 EcoO109I rG'GnC_Cy Cuts at: 0 78 1018 1098 1219 1504 1620 Size: 78 940 80 121 285 116 Fragments arranged by size: 940 285 121 116 80 78 EcoRV GAT'ATC Cuts at: 0 93 1620 Size: 93 1527 HaeII r_GCGC'y Cuts at: 0 1432 1620 Size: 1432 188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 783 815 1005 1037 1269 1288 1301 1320 Size: 783 32 190 32 232 19 13 19 Cuts at: 1320 1620 Size: 300 Fragments arranged by size: 783 300 232 190 32 32 19 19 13 HincII GTy'rAC Cuts at: 0 572 1620 Size: 572 1048 KasI G'GCGC_C Cuts at: 0 1428 1620 Size: 1428 192 MslI CAynn'nnrTG Cuts at: 0 626 1620 Size: 626 994 MspA1I CmG'CkG Cuts at: 0 1302 1620 Size: 1302 318 NarI GG'CG_CC Cuts at: 0 1429 1620 Size: 1429 191 NspI r_CATG'y Cuts at: 0 215 1620 Size: 215 1405 PflMI CCAn_nnn'nTGG Cuts at: 0 433 1323 1620 Size: 433 890 297 Fragments arranged by size: 890 433 297 PpuMI rG'GwC_Cy Cuts at: 0 1018 1504 1620 Size: 1018 486 116 Fragments arranged by size: 1018 486 116 PstI C_TGCA'G Cuts at: 0 450 798 1620 Size: 450 348 822 Fragments arranged by size: 822 450 348 PvuII CAG'CTG Cuts at: 0 1302 1620 Size: 1302 318 SexAI A'CCwGG_T Cuts at: 0 1241 1620 Size: 1241 379 SfcI C'TryA_G Cuts at: 0 446 794 931 1281 1620 Size: 446 348 137 350 339 Fragments arranged by size: 446 350 348 339 137 SfoI GGC'GCC Cuts at: 0 1430 1620 Size: 1430 190 SmlI C'TyrA_G Cuts at: 0 403 550 1620 Size: 403 147 1070 Fragments arranged by size: 1070 403 147 StyI C'CwwG_G Cuts at: 0 482 1620 Size: 482 1138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 131 1620 Size: 131 1489 TatI w'GTAC_w Cuts at: 0 946 1620 Size: 946 674 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 576 608 1620 Size: 576 32 1012 Fragments arranged by size: 1012 576 32 XmnI GAAnn'nnTTC Cuts at: 0 1059 1620 Size: 1059 561 Enzymes that do cut: AccI AcuI AflII AlwNI ApoI BamHI BanI BbeI BbsI BbvCI BciVI BclI BglII BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsmI BsrDI BstYI BstZ17I Bsu36I BtsI DraI EarI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KasI MslI MspA1I NarI NspI PflMI PpuMI PstI PvuII SexAI SfcI SfoI SmlI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanII BcgI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI