(Circular) (Six-base) MAPSORT of: pFN21AE1870.seq Check: 4505 from: 1 to: 5878 pFN21AE1870 5878 bp With 182 enzymes: SgfI * March 28, 2012 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2077 2130 3221 1937 Size: 140 53 1091 4594 Fragments arranged by size: 4594 1091 140 53 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 2631 4005 4378 1563 Size: 1068 1374 373 3063 Fragments arranged by size: 3063 1374 1068 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 3941 5325 5593 1632 Size: 863 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 863 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 2789 1166 Size: 1623 4255 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 2921 3621 5193 2921 Size: 700 1572 3606 Fragments arranged by size: 3606 1572 700 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2476 2616 2715 3187 3413 3565 5674 1493 Size: 983 140 99 472 226 152 2109 1697 Fragments arranged by size: 2109 1697 983 472 226 152 140 99 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 3203 1870 Size: 73 1260 4545 Fragments arranged by size: 4545 1260 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3199 4654 618 Size: 325 217 627 1412 1455 1842 Fragments arranged by size: 1842 1455 1412 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2234 3197 1391 Size: 622 221 963 4072 Fragments arranged by size: 4072 963 622 221 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2170 2204 4069 4103 1903 Size: 34 233 34 1865 34 3678 Fragments arranged by size: 3678 1865 233 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 3939 5095 1790 Size: 2149 1156 2573 Fragments arranged by size: 2573 2149 1156 BmgBI CAC'GTC Cuts at: 1318 2791 1318 Size: 1473 4405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 3054 4538 1239 Size: 806 1009 1484 2579 Fragments arranged by size: 2579 1484 1009 806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4003 4868 5166 4003 Size: 865 298 4715 Fragments arranged by size: 4715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2388 4541 882 Size: 617 889 2153 2219 Fragments arranged by size: 2219 2153 889 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 2742 5872 2742 Size: 3130 2748 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5878 BseYI C'CCAG_C Cuts at: 5081 5577 5081 Size: 496 5382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2067 2966 1163 Size: 573 331 899 4075 Fragments arranged by size: 4075 899 573 331 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3939 4024 5095 3939 Size: 85 1071 4722 Fragments arranged by size: 4722 1071 85 BsmI GAATG_Cn' Cuts at: 2469 3389 3482 2469 Size: 920 93 4865 Fragments arranged by size: 4865 920 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2234 3197 3939 4024 5095 1391 Size: 399 223 221 963 742 85 1071 2174 Fragments arranged by size: 2174 1071 963 742 399 223 221 85 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2089 2938 3233 844 Size: 1245 849 295 3489 Fragments arranged by size: 3489 1245 849 295 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2604 2819 4373 4547 65 Size: 1183 1356 215 1554 174 1396 Fragments arranged by size: 1554 1396 1356 1183 215 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2262 4522 4709 1700 Size: 110 452 2260 187 2869 Fragments arranged by size: 2869 2260 452 187 110 BsrGI T'GTAC_A Cuts at: 96 2578 96 Size: 2482 3396 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstYI r'GATC_y Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873 Size: 1236 367 385 17 1452 86 12 346 Cuts at: 5873 1972 Size: 1977 Fragments arranged by size: 1977 1452 1236 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2078 2131 2078 Size: 53 5825 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 3394 4188 4208 3394 Size: 794 20 5064 Fragments arranged by size: 5064 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2260 3325 4214 Size: 54 1425 50 275 18 430 1065 889 Cuts at: 4214 5843 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1065 889 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 2841 3808 1353 Size: 834 654 967 3423 Fragments arranged by size: 3423 967 834 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2495 3054 3941 4538 5325 5593 Size: 393 413 450 559 887 597 787 268 Cuts at: 5593 1239 Size: 1524 Fragments arranged by size: 1524 887 787 597 559 450 413 393 268 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2789 2789 Size: 5878 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 5025 1678 Size: 3347 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 4519 4551 4593 Size: 32 135 32 620 32 2295 32 42 Cuts at: 4593 4625 1373 Size: 32 2626 Fragments arranged by size: 2626 2295 620 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2110 3222 3468 669 Size: 988 281 172 1112 246 3079 Fragments arranged by size: 3079 1112 988 281 246 172 HindIII A'AGCT_T Cuts at: 748 2507 2696 748 Size: 1759 189 3930 Fragments arranged by size: 3930 1759 189 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 2172 3477 2172 Size: 1305 4573 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2480 4992 5176 1305 Size: 1175 2512 184 2007 Fragments arranged by size: 2512 2007 1175 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4056 Fragments arranged by size: 4056 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 3836 4195 4354 518 Size: 648 1623 1047 359 159 2042 Fragments arranged by size: 2042 1623 1047 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 3294 3971 5119 5364 1606 Size: 431 1257 677 1148 245 2120 Fragments arranged by size: 2120 1257 1148 677 431 245 NaeI GCC'GGC Cuts at: 2264 2264 Size: 5878 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NgoMIV G'CCGG_C Cuts at: 2262 2262 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2053 3146 3236 4781 1132 Size: 97 824 1093 90 1545 2229 Fragments arranged by size: 2229 1545 1093 824 97 90 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 2710 2938 3230 830 Size: 1880 228 292 3478 Fragments arranged by size: 3478 1880 292 228 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SapI GCTCTTCn'nnn_ Cuts at: 2841 2841 Size: 5878 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 2706 2934 3226 4361 5042 5233 826 Size: 219 1661 228 292 1135 681 191 1471 Fragments arranged by size: 1661 1471 1135 681 292 228 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3982 4883 5145 820 Size: 197 926 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 2053 3236 1132 Size: 921 1183 3774 Fragments arranged by size: 3774 1183 921 SspI AAT'ATT Cuts at: 5 52 2644 3802 5 Size: 47 2592 1158 2081 Fragments arranged by size: 2592 2081 1158 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2004 3058 3317 513 Size: 552 939 1054 259 3074 Fragments arranged by size: 3074 1054 939 552 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3905 3922 4075 4260 4705 325 Size: 1255 2325 17 153 185 445 1498 Fragments arranged by size: 2325 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2578 4124 5836 Size: 274 80 33 51 1443 601 1546 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 1712 1546 1443 601 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 2917 1316 Size: 1601 4277 XbaI T'CTAG_A Cuts at: 2311 3214 2311 Size: 903 4975 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 2297 2715 4007 2297 Size: 418 1292 4168 Fragments arranged by size: 4168 1292 418 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI