(Linear) (Six-base) MAPSORT of: KIEE4049.seq Check: 4243 from: 1 to: 834 KIEE4049.orf With 182 enzymes: SgfI * March 22, 2012 17:15 .. AatII G_ACGT'C Cuts at: 0 416 834 Size: 416 418 AfeI AGC'GCT Cuts at: 0 678 834 Size: 678 156 AflIII A'CryG_T Cuts at: 0 662 834 Size: 662 172 AlwNI CAG_nnn'CTG Cuts at: 0 210 834 Size: 210 624 ApaI G_GGCC'C Cuts at: 0 228 834 Size: 228 606 ApaLI G'TGCA_C Cuts at: 0 67 834 Size: 67 767 AvaI C'yCGr_G Cuts at: 0 8 282 476 629 834 Size: 8 274 194 153 205 Fragments arranged by size: 274 205 194 153 8 BanI G'GyrC_C Cuts at: 0 124 834 Size: 124 710 BanII G_rGCy'C Cuts at: 0 8 228 834 Size: 8 220 606 Fragments arranged by size: 606 220 8 BbeI G_GCGC'C Cuts at: 0 128 834 Size: 128 706 Bme1580I G_kGCm'C Cuts at: 0 71 228 477 834 Size: 71 157 249 357 Fragments arranged by size: 357 249 157 71 BmgBI CAC'GTC Cuts at: 0 39 834 Size: 39 795 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 796 834 Size: 796 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 768 834 Size: 768 66 BsaAI yAC'GTr Cuts at: 0 665 834 Size: 665 169 BsaBI GATnn'nnATC Cuts at: 0 601 834 Size: 601 233 BsaHI Gr'CG_yC Cuts at: 0 125 413 764 834 Size: 125 288 351 70 Fragments arranged by size: 351 288 125 70 BseYI C'CCAG_C Cuts at: 0 80 220 834 Size: 80 140 614 Fragments arranged by size: 614 140 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 223 346 445 834 Size: 151 72 123 99 389 Fragments arranged by size: 389 151 123 99 72 BsiHKAI G_wGCw'C Cuts at: 0 71 834 Size: 71 763 BsmI GAATG_Cn' Cuts at: 0 534 834 Size: 534 300 Bsp1286I G_dGCh'C Cuts at: 0 8 71 228 477 834 Size: 8 63 157 249 357 Fragments arranged by size: 357 249 157 63 8 BspHI T'CATG_A Cuts at: 0 543 834 Size: 543 291 BsrBI CCG'CTC Cuts at: 0 57 122 264 380 834 Size: 57 65 142 116 454 Fragments arranged by size: 454 142 116 65 57 BsrGI T'GTAC_A Cuts at: 0 344 834 Size: 344 490 BssSI C'ACGA_G Cuts at: 0 70 364 834 Size: 70 294 470 Fragments arranged by size: 470 294 70 BstAPI GCAn_nnn'nTGC Cuts at: 0 210 834 Size: 210 624 BstEII G'GTnAC_C Cuts at: 0 141 834 Size: 141 693 BtgI C'CryG_G Cuts at: 0 146 639 834 Size: 146 493 195 Fragments arranged by size: 493 195 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 284 834 Size: 284 550 EaeI y'GGCC_r Cuts at: 0 566 834 Size: 566 268 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 31 834 Size: 31 803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 796 834 Size: 796 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 364 834 Size: 364 470 EcoO109I rG'GnC_Cy Cuts at: 0 224 452 834 Size: 224 228 382 Fragments arranged by size: 382 228 224 HaeII r_GCGC'y Cuts at: 0 128 680 834 Size: 128 552 154 Fragments arranged by size: 552 154 128 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 808 834 Size: 808 26 HincII GTy'rAC Cuts at: 0 333 585 834 Size: 333 252 249 Fragments arranged by size: 333 252 249 HindIII A'AGCT_T Cuts at: 0 538 834 Size: 538 296 KasI G'GCGC_C Cuts at: 0 124 834 Size: 124 710 MslI CAynn'nnrTG Cuts at: 0 595 834 Size: 595 239 MspA1I CmG'CkG Cuts at: 0 84 102 111 207 641 834 Size: 84 18 9 96 434 193 Fragments arranged by size: 434 193 96 84 18 9 NarI GG'CG_CC Cuts at: 0 125 834 Size: 125 709 PmlI CAC'GTG Cuts at: 0 665 834 Size: 665 169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 369 401 834 Size: 369 32 433 Fragments arranged by size: 433 369 32 PpuMI rG'GwC_Cy Cuts at: 0 452 834 Size: 452 382 PspOMI G'GGCC_C Cuts at: 0 224 834 Size: 224 610 PstI C_TGCA'G Cuts at: 0 101 185 739 834 Size: 101 84 554 95 Fragments arranged by size: 554 101 95 84 PvuII CAG'CTG Cuts at: 0 102 111 207 834 Size: 102 9 96 627 Fragments arranged by size: 627 102 96 9 SacII CC_GC'GG Cuts at: 0 642 834 Size: 642 192 SanDI GG'GwC_CC Cuts at: 0 452 834 Size: 452 382 ScaI AGT'ACT Cuts at: 0 700 834 Size: 700 134 SexAI A'CCwGG_T Cuts at: 0 137 834 Size: 137 697 SfcI C'TryA_G Cuts at: 0 97 181 735 834 Size: 97 84 554 99 Fragments arranged by size: 554 99 97 84 SfoI GGC'GCC Cuts at: 0 126 834 Size: 126 708 SmaI CCC'GGG Cuts at: 0 10 478 631 834 Size: 10 468 153 203 Fragments arranged by size: 468 203 153 10 SmlI C'TyrA_G Cuts at: 0 747 834 Size: 747 87 StuI AGG'CCT Cuts at: 0 370 834 Size: 370 464 StyI C'CwwG_G Cuts at: 0 326 569 834 Size: 326 243 265 Fragments arranged by size: 326 265 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 39 834 Size: 39 795 TatI w'GTAC_w Cuts at: 0 344 698 834 Size: 344 354 136 Fragments arranged by size: 354 344 136 Tth111I GACn'n_nGTC Cuts at: 0 493 723 834 Size: 493 230 111 Fragments arranged by size: 493 230 111 XmaI C'CCGG_G Cuts at: 0 8 476 629 834 Size: 8 468 153 205 Fragments arranged by size: 468 205 153 8 ZraI GAC'GTC Cuts at: 0 414 834 Size: 414 420 Enzymes that do cut: AatII AfeI AflIII AlwNI ApaI ApaLI AvaI BanI BanII BbeI Bme1580I BmgBI BpmI BpuEI BsaAI BsaBI BsaHI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrGI BssSI BstAPI BstEII BtgI BtgZI EaeI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI MslI MspA1I NarI PmlI PpiI PpuMI PspOMI PstI PvuII SacII SanDI ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BspMI BsrDI BsrFI BssHII BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XcmI XhoI XmnI