################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbsI BbvCI BciVI BclI BfaI BfrI BlnI BpuAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcyI 352 GGCGCC G[GA]^CG[CT]C Alw44I 367 GTGCAC G^TGCAC AlwI 281 GGATC GGATC(4/5) ApaLI 367 GTGCAC G^TGCAC AspHI 367 GTGCAC G[AT]GC[AT]^C AspI 475 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 352 GGCGCC G^G[CT][GA]CC BanII 168 GGGCTC G[GA]GC[CT]^C BbeI 352 GGCGCC GGCGC^C BbrPI 370 CACGTG CAC^GTG BcgI 43 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 204 CCGGG CC^[GC]GG BglII 533 AGATCT A^GATCT BlpI 130 GCTCAGC GC^T[ACGT]AGC BpmI 418 CTGGAG CTGGAG(16/14) Bpu1102I 130 GCTCAGC GC^T[ACGT]AGC BsaAI 370 CACGTG [CT]AC^GT[GA] BsaHI 352 GGCGCC G[GA]^CG[CT]C BsaI 385 GAGACC GGTCTC(1/5) BseRI 600 GAGGAG GAGGAG(10/8) BsiEI 327 CGGCCG CG[GA][CT]^CG BsiHKAI 367 GTGCAC G[AT]GC[AT]^C BspMI 291 GCAGGT ACCTGC(4/8) BsrFI 80 GCCGGC [GA]^CCGG[CT] BsrI 576 CCAGT ACTGG(1/-1) BstXI 300 CCAGCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 130 GCTCAGC GC^T[ACGT]AGC Cfr10I 80 GCCGGC [GA]^CCGG[CT] DrdI 313 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 248 CCATGG C^C[GA][CT]GG EaeI 327 CGGCCG [CT]^GGCC[GA] EagI 327 CGGCCG C^GGCCG EclXI 327 CGGCCG C^GGCCG Eco52I 327 CGGCCG C^GGCCG EcoNI 519 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 352 GGCGCC [GA]GCGC^[CT] Hin1I 352 GGCGCC G[GA]^CG[CT]C KasI 352 GGCGCC G^GCGCC MboII 536 TCTTC GAAGA(8/7) MslI 243 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 80 GCCGGC GCC^GGC NarI 352 GGCGCC GG^CGCC NciI 204 CCGGG CC^[GC]GG NcoI 248 CCATGG C^CATGG NgoMIV 80 GCCGGC G^CCGGC NspI 225 GCATGT [GA]CATG^[CT] PflFI 475 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 582 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 370 CACGTG CAC^GTG PmlI 370 CACGTG CAC^GTG SbfI 186 CCTGCAGG CCTGCA^GG SfiI 74 GGCCACGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 352 GGCGCC GGC^GCC Sse8387I 186 CCTGCAGG CCTGCA^GG Tsp509I 121 AATT ^AATT Tth111I 475 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 582 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 218 CCACACTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AvaI 347 CCCGAG C^[CT]CG[GA]G 356 CCCGAG BsmAI 311 GAGAC GTCTC(1/5) 385 GAGAC BsmFI 13 GGGAC GGGAC(10/14) 145 GGGAC BsoBI 347 CCCGAG C^[CT]CG[GA]G 356 CCCGAG BsrBI 456 CCGCTC CCGCTC(-3/-3) 421 GAGCGG BstYI 281 GGATCT [GA]^GATC[CT] 533 AGATCT CfoI 297 GCGC GCG^C 353 GCGC DpnII 282 GATC ^GATC 534 GATC DraII 145 GGGACCT [GA]G^G[ACGT]CC[CT] 320 GGGTCCT EcoO109I 145 GGGACCT [GA]G^G[ACGT]CC[CT] 320 GGGTCCT EcoT14I 113 CCAAGG C^C[AT][AT]GG 248 CCATGG HapII 81 CCGG C^CGG 204 CCGG HhaI 297 GCGC GCG^C 353 GCGC HinP1I 297 GCGC G^CGC 353 GCGC HinfI 30 GACTC G^A[ACGT]TC 475 GACTC HpaII 81 CCGG C^CGG 204 CCGG MaeII 371 ACGT A^CGT 584 ACGT MboI 282 GATC ^GATC 534 GATC MflI 281 GGATCT [GA]^GATC[CT] 533 AGATCT MspI 81 CCGG C^CGG 204 CCGG NdeII 282 GATC ^GATC 534 GATC NlaIII 226 CATG CATG^ 249 CATG PleI 30 GACTC GAGTC(4/5) 475 GACTC PpuMI 145 GGGACCT [GA]G^G[AT]CC[CT] 320 GGGTCCT PstI 187 CTGCAG CTGCA^G 569 CTGCAG PvuII 44 CAGCTG CAG^CTG 433 CAGCTG Sau3AI 282 GATC ^GATC 534 GATC SfaNI 127 GATGC GCATC(5/9) 88 GCATC SfcI 187 CTGCAG C^T[GA][CT]AG 569 CTGCAG StyI 113 CCAAGG C^C[AT][AT]GG 248 CCATGG TspRI 221 CACTG CA[GC]TG[ACGT][ACGT]^ 440 CAGTG XhoII 281 GGATCT [GA]^GATC[CT] 533 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BglI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII ItaI MnlI MspA1I MvaI MwoI NlaIV RsaI Sau96I ScrFI TseI