################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbvCI BciVI BclI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstXI BstZ17I Bsu36I CpoI DraI DrdI Eam1105I EarI Eco81I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 1429 GACGTC GACGT^C AcsI 1648 GAATTC [GA]^AATT[CT] Alw44I 1438 GTGCAC G^TGCAC Aor51HI 1841 AGCGCT AGC^GCT ApaLI 1438 GTGCAC G^TGCAC ApoI 1648 GAATTC [GA]^AATT[CT] BbrPI 1441 CACGTG CAC^GTG BfaI 85 CTAG C^TAG BglII 1070 AGATCT A^GATCT BspDI 1831 ATCGAT AT^CGAT BsrBI 1273 GAGCGG CCGCTC(-3/-3) BssHII 918 GCGCGC G^CGCGC BssSI 1452 CACGAG CACGAG(-5/-1) BstAPI 1134 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 298 GGTGACC G^GT[ACGT]ACC BstPI 298 GGTGACC G^GT[ACGT]ACC ClaI 1831 ATCGAT AT^CGAT DraIII 191 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1841 AGCGCT AGC^GCT EcoO65I 298 GGTGACC G^GT[ACGT]ACC EcoRI 1648 GAATTC G^AATTC HincII 1503 GTTGAC GT[CT]^[GA]AC HindII 1503 GTTGAC GT[CT]^[GA]AC KspI 20 CCGCGG CCGC^GG MaeI 85 CTAG C^TAG MaeIII 299 GTGAC ^GT[ACGT]AC NspI 1802 ACATGC [GA]CATG^[CT] PmaCI 1441 CACGTG CAC^GTG PmlI 1441 CACGTG CAC^GTG SacII 20 CCGCGG CCGC^GG ScaI 809 AGTACT AGT^ACT SfiI 1200 GGCCCAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 346 CTCAAG C^T[CT][GA]AG TfiI 1730 GAATC G^A[AT]TC Tsp45I 299 GTGAC ^GT[GC]AC Tsp509I 1649 AATT ^AATT XcmI 323 CCAGTGGAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflIII 461 ACGTGT A^C[GA][CT]GT 1016 ACGTGT BalI 707 TGGCCA TGG^CCA 1557 TGGCCA BbeI 53 GGCGCC GGCGC^C 1028 GGCGCC BcgI 366 CGACGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 828 GCAACTGTCTCG BglI 209 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1201 GCCCAGGGGGC BlpI 1233 GCTGAGC GC^T[ACGT]AGC 1924 GCTAAGC Bpu1102I 1233 GCTGAGC GC^T[ACGT]AGC 1924 GCTAAGC BsaAI 1015 TACGTG [CT]AC^GT[GA] 1441 CACGTG BsaI 547 GAGACC GGTCTC(1/5) 1243 GAGACC BsgI 163 GTGCAG GTGCAG(16/14) 1918 GTGCAG BsrFI 1189 GCCGGC [GA]^CCGG[CT] 1682 GCCGGC BstF5I 1458 CATCC GGATG(2/0) 68 GGATG BstYI 1070 AGATCT [GA]^GATC[CT] 1950 GGATCT CelII 1233 GCTGAGC GC^T[ACGT]AGC 1924 GCTAAGC Cfr10I 1189 GCCGGC [GA]^CCGG[CT] 1682 GCCGGC EcoNI 552 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 591 CCTGGTGAAGG FokI 1458 CATCC GGATG(9/13) 68 GGATG KasI 53 GGCGCC G^GCGCC 1028 GGCGCC MflI 1070 AGATCT [GA]^GATC[CT] 1950 GGATCT MluNI 707 TGGCCA TGG^CCA 1557 TGGCCA MscI 707 TGGCCA TGG^CCA 1557 TGGCCA NaeI 1189 GCCGGC GCC^GGC 1682 GCCGGC NarI 53 GGCGCC GG^CGCC 1028 GGCGCC NcoI 1022 CCATGG C^CATGG 1095 CCATGG NgoMIV 1189 GCCGGC G^CCGGC 1682 GCCGGC PflMI 852 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 957 CCAGGAGCTGG PleI 1262 GACTC GAGTC(4/5) 1330 GACTC SacI 31 GAGCTC GAGCT^C 1102 GAGCTC SfoI 53 GGCGCC GGC^GCC 1028 GGCGCC StuI 560 AGGCCT AGG^CCT 869 AGGCCT Van91I 852 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 957 CCAGGAGCTGG XhoII 1070 AGATCT [GA]^GATC[CT] 1950 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TseI TspRI TthHB8I XmaCI XmaI