(Circular) (Six-base) MAPSORT of: pFN21AE3272.seq Check: 1289 from: 1 to: 6730 pFN21AE3272 6730 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2161 2541 2949 2958 3078 2161 Size: 380 408 9 120 5813 Fragments arranged by size: 5813 408 380 120 9 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3438 4540 278 Size: 53 83 186 936 1902 1102 2468 Fragments arranged by size: 2468 1902 1102 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4073 1937 Size: 2136 4594 Acc65I G'GTAC_C Cuts at: 4051 4051 Size: 6730 AclI AA'CG_TT Cuts at: 1563 4857 5230 1563 Size: 3294 373 3063 Fragments arranged by size: 3294 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2261 3889 4793 6177 6445 1632 Size: 578 51 1628 904 1384 268 1917 Fragments arranged by size: 1917 1628 1384 904 578 268 51 AfeI AGC'GCT Cuts at: 3848 3848 Size: 6730 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6533 AflIII A'CryG_T Cuts at: 1203 2466 3021 5629 6606 1203 Size: 1263 555 2608 977 1327 Fragments arranged by size: 2608 1327 1263 977 555 AgeI A'CCGG_T Cuts at: 5561 5561 Size: 6730 AhdI GACnn_n'nnGTC Cuts at: 5459 5459 Size: 6730 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6730 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6730 AlwNI CAG_nnn'CTG Cuts at: 2968 3049 3238 4473 6045 2968 Size: 81 189 1235 1572 3653 Fragments arranged by size: 3653 1572 1235 189 81 ApaI G_GGCC'C Cuts at: 2015 2048 2449 3327 3475 3881 3971 2015 Size: 33 401 878 148 406 90 4774 Fragments arranged by size: 4774 878 406 401 148 90 33 ApaLI G'TGCA_C Cuts at: 3443 4787 5943 3443 Size: 1344 1156 4230 Fragments arranged by size: 4230 1344 1156 ApoI r'AATT_y Cuts at: 1493 3653 4039 4265 4417 6526 1493 Size: 2160 386 226 152 2109 1697 Fragments arranged by size: 2160 2109 1697 386 226 152 AseI AT'TA_AT Cuts at: 160 1032 5284 160 Size: 872 4252 1606 Fragments arranged by size: 4252 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 AvaI C'yCGr_G Cuts at: 1870 1943 2041 2525 3399 3466 3873 3995 4055 Size: 73 98 484 874 67 407 122 60 Cuts at: 4055 1870 Size: 4545 Fragments arranged by size: 4545 874 484 407 122 98 73 67 60 BamHI G'GATC_C Cuts at: 4060 4427 4060 Size: 367 6363 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2058 2177 2529 2946 3033 Size: 325 217 627 271 119 352 417 87 Cuts at: 3033 3756 4051 5506 618 Size: 723 295 1455 1842 Fragments arranged by size: 1842 1455 723 627 417 352 325 295 271 217 119 87 BanII G_rGCy'C Cuts at: 1391 2015 2040 2048 2449 3111 3327 3475 3780 Size: 624 25 8 401 662 216 148 305 Cuts at: 3780 3881 3971 4002 4049 1391 Size: 101 90 31 47 4072 Fragments arranged by size: 4072 662 624 401 305 216 148 101 90 47 31 25 8 BbeI G_GCGC'C Cuts at: 2062 3037 2062 Size: 975 5755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2198 2776 2831 3471 928 Size: 971 299 578 55 640 4187 Fragments arranged by size: 4187 971 640 578 299 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2360 2394 2822 2856 4921 4955 1903 Size: 34 423 34 428 34 2065 34 3678 Fragments arranged by size: 3678 2065 428 423 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4622 5832 4622 Size: 1210 5520 BclI T'GATC_A Cuts at: 1548 1548 Size: 6730 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2220 3212 5341 Size: 107 122 71 631 605 548 992 2129 Cuts at: 5341 136 Size: 1525 Fragments arranged by size: 2129 1525 992 631 605 548 122 107 71 BglII A'GATC_T Cuts at: 3075 6725 3075 Size: 3650 3080 BlpI GC'TnA_GC Cuts at: 3239 3930 4147 3239 Size: 691 217 5822 Fragments arranged by size: 5822 691 217 Bme1580I G_kGCm'C Cuts at: 1790 2015 2048 2180 2449 2532 3327 3447 3475 Size: 225 33 132 269 83 795 120 28 Cuts at: 3475 3759 3881 3971 4791 5947 1790 Size: 284 122 90 820 1156 2573 Fragments arranged by size: 2573 1156 820 795 284 269 225 132 122 120 90 83 33 28 BmgBI CAC'GTC Cuts at: 1318 2782 2803 1318 Size: 1464 21 5245 Fragments arranged by size: 5245 1464 21 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4461 5419 442 Size: 416 53 83 399 24 3044 958 1753 Fragments arranged by size: 3044 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2053 2123 3022 3296 3623 3814 5390 1239 Size: 814 70 899 274 327 191 1576 2579 Fragments arranged by size: 2579 1576 899 814 327 274 191 70 Bpu10I CC'TnA_GC Cuts at: 2721 2849 2721 Size: 128 6602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 4855 5720 6018 2336 Size: 2519 865 298 3048 Fragments arranged by size: 3048 2519 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2546 3242 5393 882 Size: 617 1047 696 2151 2219 Fragments arranged by size: 2219 2151 1047 696 617 BsaAI yAC'GTr Cuts at: 493 1204 3022 3448 4521 493 Size: 711 1818 426 1073 2702 Fragments arranged by size: 2702 1818 1073 711 426 BsaBI GATnn'nnATC Cuts at: 6724 6724 Size: 6730 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2059 2081 3034 3435 Size: 53 83 186 936 526 22 953 401 Cuts at: 3435 4537 4919 5554 275 Size: 1102 382 635 1451 Fragments arranged by size: 1451 1102 953 936 635 526 401 382 186 83 53 22 BsaWI w'CCGG_w Cuts at: 5163 5561 5835 5982 6393 5163 Size: 398 274 147 411 5500 Fragments arranged by size: 5500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2415 2445 3812 3842 1178 Size: 30 165 30 1012 30 1367 30 4066 Fragments arranged by size: 4066 1367 1012 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2764 3370 3406 3503 1186 Size: 1578 606 36 97 4413 Fragments arranged by size: 4413 1578 606 97 36 BseYI C'CCAG_C Cuts at: 2218 2608 3037 3326 3618 4002 5933 6429 2218 Size: 390 429 289 292 384 1931 496 2519 Fragments arranged by size: 2519 1931 496 429 390 384 292 289 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 3944 1163 Size: 573 453 1755 3949 Fragments arranged by size: 3949 1755 573 453 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 2052 2254 2617 2931 3154 Size: 275 186 30 21 202 363 314 223 Cuts at: 3154 3591 4180 4941 5969 1540 Size: 437 589 761 1028 2301 Fragments arranged by size: 2301 1028 761 589 437 363 314 275 223 202 186 30 21 BsiHKAI G_wGCw'C Cuts at: 2040 2250 3111 3150 3447 4791 4876 5947 2040 Size: 210 861 39 297 1344 85 1071 2823 Fragments arranged by size: 2823 1344 1071 861 297 210 85 39 BsmI GAATG_Cn' Cuts at: 4241 4334 4241 Size: 93 6637 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2040 2048 2180 2250 2449 2532 Size: 399 225 25 8 132 70 199 83 Cuts at: 2532 3111 3150 3327 3447 3475 3759 3780 3881 Size: 579 39 177 120 28 284 21 101 Cuts at: 3881 3971 4002 4049 4791 4876 5947 1391 Size: 90 31 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 399 284 225 199 177 132 120 101 90 85 83 70 47 39 31 28 25 21 8 BspEI T'CCGG_A Cuts at: 6393 6393 Size: 6730 BspHI T'CATG_A Cuts at: 4619 6349 4619 Size: 1730 5000 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2161 2541 2949 2958 3078 3267 4085 844 Size: 1317 380 408 9 120 189 818 3489 Fragments arranged by size: 3489 1317 818 408 380 189 120 9 BsrBI CCG'CTC Cuts at: 1394 1823 3280 4617 1394 Size: 429 1457 1337 3507 Fragments arranged by size: 3507 1457 1337 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2715 2955 3754 5225 5399 65 Size: 1183 1467 240 799 1471 174 1396 Fragments arranged by size: 1471 1467 1396 1183 799 240 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3194 3687 5374 5561 1700 Size: 110 1384 493 1687 187 2869 Fragments arranged by size: 2869 1687 1384 493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6730 BssHII G'CGCG_C Cuts at: 1925 2923 1925 Size: 998 5732 BssSI C'ACGA_G Cuts at: 3457 4790 5802 3457 Size: 1333 1012 4385 Fragments arranged by size: 4385 1333 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3145 794 Size: 2351 4379 BstBI TT'CG_AA Cuts at: 5534 5534 Size: 6730 BstEII G'GTnAC_C Cuts at: 1184 2303 1184 Size: 1119 5611 BstYI r'GATC_y Cuts at: 1972 3075 3955 4060 4427 4812 4829 6281 6367 Size: 1103 880 105 367 385 17 1452 86 Cuts at: 6367 6379 6725 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1103 880 385 367 346 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 3027 3100 3585 3769 513 Size: 552 939 21 1002 73 485 184 3474 Fragments arranged by size: 3474 1002 939 552 485 184 73 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2392 3652 487 Size: 683 56 1166 1260 3565 Fragments arranged by size: 3565 1260 1166 683 56 BtsI GCAGTG_nn' Cuts at: 2868 4016 4246 5040 5060 2868 Size: 1148 230 794 20 4538 Fragments arranged by size: 4538 1148 794 230 20 ClaI AT'CG_AT Cuts at: 1554 3837 1554 Size: 2283 4447 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2311 2346 529 Size: 35 1747 35 4913 Fragments arranged by size: 4913 1747 35 35 DraI TTT'AAA Cuts at: 4034 4034 Size: 6730 DraIII CAC_nnn'GTG Cuts at: 2201 2201 Size: 6730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5737 809 Size: 507 4421 1802 Fragments arranged by size: 4421 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2049 2251 Size: 54 1425 50 275 18 198 21 202 Cuts at: 2251 2712 2805 2928 2970 3151 3192 3562 3588 Size: 461 93 123 42 181 41 370 26 Cuts at: 3588 4177 5066 6695 8 Size: 589 889 1629 43 Fragments arranged by size: 1629 1425 889 589 461 370 275 202 198 181 123 93 54 50 43 42 41 26 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2028 2049 2251 2928 3151 3588 4177 Size: 275 216 21 202 677 223 437 589 Cuts at: 4177 1537 Size: 4090 Fragments arranged by size: 4090 677 589 437 275 223 216 202 21 EarI CTCTTCn'nnn_ Cuts at: 1353 4660 1353 Size: 3307 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5317 5691 5837 387 Size: 1064 138 3728 374 146 1280 Fragments arranged by size: 3728 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2038 3109 2038 Size: 1071 5659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2123 2210 2261 3022 3296 3623 Size: 393 421 70 87 51 761 274 327 Cuts at: 3623 3814 3889 4793 5390 6177 6445 1239 Size: 191 75 904 597 787 268 1524 Fragments arranged by size: 1524 904 787 761 597 421 393 327 274 268 191 87 75 70 51 EcoNI CCTnn'n_nnAGG Cuts at: 2561 2600 2561 Size: 39 6691 EcoO109I rG'GnC_Cy Cuts at: 2185 2356 2445 2446 2521 2634 2754 3046 3256 Size: 171 89 1 75 113 120 292 210 Cuts at: 3256 3323 3396 3471 3472 3877 3878 3967 2185 Size: 67 73 75 1 405 1 89 4948 Fragments arranged by size: 4948 405 292 210 171 120 113 89 89 75 75 73 67 1 1 1 EcoRI G'AATT_C Cuts at: 3653 4039 4417 3653 Size: 386 378 5966 Fragments arranged by size: 5966 386 378 FspI TGC'GCA Cuts at: 5236 5236 Size: 6730 HaeII r_GCGC'y Cuts at: 1678 2062 2102 2261 2368 2710 2797 3037 3432 Size: 384 40 159 107 342 87 240 395 Cuts at: 3432 3850 5877 1678 Size: 418 2027 2531 Fragments arranged by size: 2531 2027 418 395 384 342 240 159 107 87 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5371 5403 5445 5477 1373 Size: 32 135 32 3799 32 42 32 2626 Fragments arranged by size: 3799 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3510 4074 4320 669 Size: 988 281 1572 564 246 3079 Fragments arranged by size: 3079 1572 988 564 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6730 HpaI GTT'AAC Cuts at: 4320 4320 Size: 6730 KasI G'GCGC_C Cuts at: 2058 3033 2058 Size: 975 5755 KpnI G_GTAC'C Cuts at: 4055 4055 Size: 6730 MfeI C'AATT_G Cuts at: 4329 4329 Size: 6730 MluI A'CGCG_T Cuts at: 6606 6606 Size: 6730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5844 6028 1305 Size: 4539 184 2007 Fragments arranged by size: 4539 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 3564 10 Size: 54 1425 343 882 850 3176 Fragments arranged by size: 3176 1425 882 850 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 3125 3747 4688 5047 5206 518 Size: 648 1108 851 622 941 359 159 2042 Fragments arranged by size: 2042 1108 941 851 648 622 359 159 MspA1I CmG'CkG Cuts at: 1606 2027 2218 2395 3136 3142 3421 3703 3949 Size: 421 191 177 741 6 279 282 246 Cuts at: 3949 4146 4823 5971 6216 1606 Size: 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 741 677 421 282 279 246 245 197 191 177 6 NaeI GCC'GGC Cuts at: 3196 3689 3196 Size: 493 6237 NarI GG'CG_CC Cuts at: 2059 3034 2059 Size: 975 5755 NcoI C'CATG_G Cuts at: 513 1065 2004 3027 3100 513 Size: 552 939 1023 73 4143 Fragments arranged by size: 4143 1023 939 552 73 NdeI CA'TA_TG Cuts at: 387 387 Size: 6730 NgoMIV G'CCGG_C Cuts at: 3194 3687 3194 Size: 493 6237 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6730 NotI GC'GGCC_GC Cuts at: 4177 4177 Size: 6730 NspI r_CATG'y Cuts at: 1132 1229 3811 4088 5633 1132 Size: 97 2582 277 1545 2229 Fragments arranged by size: 2582 2229 1545 277 97 PasI CC'CwG_GG Cuts at: 1348 3208 1348 Size: 1860 4870 PciI A'CATG_T Cuts at: 5629 5629 Size: 6730 PflMI CCAn_nnn'nTGG Cuts at: 1108 2863 2968 1108 Size: 1755 105 4870 Fragments arranged by size: 4870 1755 105 PfoI T'CCnGG_A Cuts at: 2069 2495 3830 2069 Size: 426 1335 4969 Fragments arranged by size: 4969 1335 426 PmeI GTTT'AAAC Cuts at: 4034 4034 Size: 6730 PmlI CAC'GTG Cuts at: 3448 3448 Size: 6730 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4799 4831 6312 6344 4799 Size: 32 1481 32 5185 Fragments arranged by size: 5185 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2185 2356 2521 3256 2185 Size: 171 165 735 5659 Fragments arranged by size: 5659 735 171 165 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6730 PsiI TTA'TAA Cuts at: 4300 4300 Size: 6730 PspOMI G'GGCC_C Cuts at: 2011 2044 2445 3323 3471 3877 3967 2011 Size: 33 401 878 148 406 90 4774 Fragments arranged by size: 4774 878 406 401 148 90 33 PstI C_TGCA'G Cuts at: 830 2913 3075 3141 4082 830 Size: 2083 162 66 941 3478 Fragments arranged by size: 3478 2083 941 162 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6730 PvuII CAG'CTG Cuts at: 2218 3136 3142 2218 Size: 918 6 5806 Fragments arranged by size: 5806 918 6 SacI G_AGCT'C Cuts at: 2040 3111 2040 Size: 1071 5659 SacII CC_GC'GG Cuts at: 2028 2028 Size: 6730 SalI G'TCGA_C Cuts at: 1936 4072 1936 Size: 2136 4594 SbfI CC_TGCA'GG Cuts at: 4082 4082 Size: 6730 ScaI AGT'ACT Cuts at: 2816 4978 6690 2816 Size: 2162 1712 2856 Fragments arranged by size: 2856 2162 1712 SfcI C'TryA_G Cuts at: 826 1045 2209 2758 2881 2909 3071 3137 4078 Size: 219 1164 549 123 28 162 66 941 Cuts at: 4078 5213 5894 6085 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1164 1135 941 681 549 219 191 162 123 66 28 SfiI GGCCn_nnn'nGGCC Cuts at: 3212 3212 Size: 6730 SfoI GGC'GCC Cuts at: 2060 3035 2060 Size: 975 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6730 SmaI CCC'GGG Cuts at: 1872 2043 2527 3401 3875 4057 1872 Size: 171 484 874 474 182 4545 Fragments arranged by size: 4545 874 484 474 182 171 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 4834 5735 5997 820 Size: 197 926 408 2483 901 262 1553 Fragments arranged by size: 2483 1553 926 901 408 262 197 SnaBI TAC'GTA Cuts at: 493 4521 493 Size: 4028 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6730 SphI G_CATG'C Cuts at: 1132 4088 1132 Size: 2956 3774 SspI AAT'ATT Cuts at: 5 52 4654 5 Size: 47 4602 2081 Fragments arranged by size: 4602 2081 47 StuI AGG'CCT Cuts at: 1517 2567 2876 1517 Size: 1050 309 5371 Fragments arranged by size: 5371 1050 309 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 3027 3100 4169 513 Size: 552 939 318 705 73 1069 3074 Fragments arranged by size: 3074 1069 939 705 552 318 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2672 3662 3755 4757 4774 4927 5112 Size: 1255 1092 990 93 1002 17 153 185 Cuts at: 5112 5557 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1092 1002 990 445 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 4976 6688 Size: 274 80 33 51 1443 837 2162 1712 Cuts at: 6688 96 Size: 138 Fragments arranged by size: 2162 1712 1443 837 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2287 2319 2340 2372 3476 3508 6690 6722 2287 Size: 32 21 32 1104 32 3182 32 2295 Fragments arranged by size: 3182 2295 1104 32 32 32 32 21 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6730 XbaI T'CTAG_A Cuts at: 4066 4066 Size: 6730 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2335 2335 Size: 6730 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6730 XmaI C'CCGG_G Cuts at: 1870 2041 2525 3399 3873 4055 1870 Size: 171 484 874 474 182 4545 Fragments arranged by size: 4545 874 484 474 182 171 XmnI GAAnn'nnTTC Cuts at: 4859 4859 Size: 6730 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3436 4538 276 Size: 53 83 186 936 1902 1102 2468 Fragments arranged by size: 2468 1902 1102 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI