(Linear) (Six-base) MAPSORT of: KIEE3272.seq Check: 1985 from: 1 to: 2025 KIEE3272.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 156 536 944 953 1073 2025 Size: 156 380 408 9 120 952 Fragments arranged by size: 952 408 380 156 120 9 AatII G_ACGT'C Cuts at: 0 1433 2025 Size: 1433 592 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 256 1884 2025 Size: 205 51 1628 141 Fragments arranged by size: 1628 205 141 51 AfeI AGC'GCT Cuts at: 0 1843 2025 Size: 1843 182 AflIII A'CryG_T Cuts at: 0 461 1016 2025 Size: 461 555 1009 Fragments arranged by size: 1009 555 461 AlwNI CAG_nnn'CTG Cuts at: 0 963 1044 1233 2025 Size: 963 81 189 792 Fragments arranged by size: 963 792 189 81 ApaI G_GGCC'C Cuts at: 0 10 43 444 1322 1470 1876 1966 2025 Size: 10 33 401 878 148 406 90 59 Fragments arranged by size: 878 406 401 148 90 59 33 10 ApaLI G'TGCA_C Cuts at: 0 1438 2025 Size: 1438 587 ApoI r'AATT_y Cuts at: 0 1648 2025 Size: 1648 377 AvaI C'yCGr_G Cuts at: 0 36 520 1394 1461 1868 1990 2025 Size: 36 484 874 67 407 122 35 Fragments arranged by size: 874 484 407 122 67 36 35 BanI G'GyrC_C Cuts at: 0 53 172 524 941 1028 1751 2025 Size: 53 119 352 417 87 723 274 Fragments arranged by size: 723 417 352 274 119 87 53 BanII G_rGCy'C Cuts at: 0 10 35 43 444 1106 1322 1470 1775 Size: 10 25 8 401 662 216 148 305 Cuts at: 1775 1876 1966 1997 2025 Size: 101 90 31 28 Fragments arranged by size: 662 401 305 216 148 101 90 31 28 25 10 8 BbeI G_GCGC'C Cuts at: 0 57 1032 2025 Size: 57 975 993 Fragments arranged by size: 993 975 57 BbsI GAAGACnn'nnnn_ Cuts at: 0 193 771 826 1466 2025 Size: 193 578 55 640 559 Fragments arranged by size: 640 578 559 193 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 355 389 817 851 2025 Size: 355 34 428 34 1174 Fragments arranged by size: 1174 428 355 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 215 1207 2025 Size: 215 992 818 Fragments arranged by size: 992 818 215 BglII A'GATC_T Cuts at: 0 1070 2025 Size: 1070 955 BlpI GC'TnA_GC Cuts at: 0 1234 1925 2025 Size: 1234 691 100 Fragments arranged by size: 1234 691 100 Bme1580I G_kGCm'C Cuts at: 0 10 43 175 444 527 1322 1442 1470 Size: 10 33 132 269 83 795 120 28 Cuts at: 1470 1754 1876 1966 2025 Size: 284 122 90 59 Fragments arranged by size: 795 284 269 132 122 120 90 83 59 33 28 10 BmgBI CAC'GTC Cuts at: 0 777 798 2025 Size: 777 21 1227 Fragments arranged by size: 1227 777 21 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 118 1017 1291 1618 1809 2025 Size: 48 70 899 274 327 191 216 Fragments arranged by size: 899 327 274 216 191 70 48 Bpu10I CC'TnA_GC Cuts at: 0 716 844 2025 Size: 716 128 1181 Fragments arranged by size: 1181 716 128 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 2025 Size: 331 1694 BsaI GGTCTCn'nnnn_ Cuts at: 0 541 1237 2025 Size: 541 696 788 Fragments arranged by size: 788 696 541 BsaAI yAC'GTr Cuts at: 0 1017 1443 2025 Size: 1017 426 582 Fragments arranged by size: 1017 582 426 BsaHI Gr'CG_yC Cuts at: 0 54 76 1029 1430 2025 Size: 54 22 953 401 595 Fragments arranged by size: 953 595 401 54 22 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 410 440 1807 1837 2025 Size: 410 30 1367 30 188 Fragments arranged by size: 1367 410 188 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 759 1365 1401 1498 2025 Size: 759 606 36 97 527 Fragments arranged by size: 759 606 527 97 36 BseYI C'CCAG_C Cuts at: 0 213 603 1032 1321 1613 1997 2025 Size: 213 390 429 289 292 384 28 Fragments arranged by size: 429 390 384 292 289 213 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 1939 2025 Size: 184 1755 86 Fragments arranged by size: 1755 184 86 BsiEI CG_ry'CG Cuts at: 0 26 47 249 612 926 1149 1586 2025 Size: 26 21 202 363 314 223 437 439 Fragments arranged by size: 439 437 363 314 223 202 26 21 BsiHKAI G_wGCw'C Cuts at: 0 35 245 1106 1145 1442 2025 Size: 35 210 861 39 297 583 Fragments arranged by size: 861 583 297 210 39 35 Bsp1286I G_dGCh'C Cuts at: 0 10 35 43 175 245 444 527 1106 Size: 10 25 8 132 70 199 83 579 Cuts at: 1106 1145 1322 1442 1470 1754 1775 1876 1966 Size: 39 177 120 28 284 21 101 90 Cuts at: 1966 1997 2025 Size: 31 28 Fragments arranged by size: 579 284 199 177 132 120 101 90 83 70 39 31 28 28 25 21 10 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 156 536 944 953 1073 1262 2025 Size: 156 380 408 9 120 189 763 Fragments arranged by size: 763 408 380 189 156 120 9 BsrBI CCG'CTC Cuts at: 0 1275 2025 Size: 1275 750 BsrDI GCAATG_nn' Cuts at: 0 710 950 1749 2025 Size: 710 240 799 276 Fragments arranged by size: 799 710 276 240 BsrFI r'CCGG_y Cuts at: 0 1189 1682 2025 Size: 1189 493 343 Fragments arranged by size: 1189 493 343 BssHII G'CGCG_C Cuts at: 0 918 2025 Size: 918 1107 BssSI C'ACGA_G Cuts at: 0 1452 2025 Size: 1452 573 BstAPI GCAn_nnn'nTGC Cuts at: 0 1140 2025 Size: 1140 885 BstEII G'GTnAC_C Cuts at: 0 298 2025 Size: 298 1727 BstYI r'GATC_y Cuts at: 0 1070 1950 2025 Size: 1070 880 75 Fragments arranged by size: 1070 880 75 BtgI C'CryG_G Cuts at: 0 20 1022 1095 1580 1764 2025 Size: 20 1002 73 485 184 261 Fragments arranged by size: 1002 485 261 184 73 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 387 1647 2025 Size: 387 1260 378 Fragments arranged by size: 1260 387 378 BtsI GCAGTG_nn' Cuts at: 0 863 2011 2025 Size: 863 1148 14 Fragments arranged by size: 1148 863 14 ClaI AT'CG_AT Cuts at: 0 1832 2025 Size: 1832 193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 306 341 2025 Size: 306 35 1684 Fragments arranged by size: 1684 306 35 DraIII CAC_nnn'GTG Cuts at: 0 196 2025 Size: 196 1829 EaeI y'GGCC_r Cuts at: 0 23 44 246 707 800 923 965 1146 Size: 23 21 202 461 93 123 42 181 Cuts at: 1146 1187 1557 1583 2025 Size: 41 370 26 442 Fragments arranged by size: 461 442 370 202 181 123 93 42 41 26 23 21 EagI C'GGCC_G Cuts at: 0 23 44 246 923 1146 1583 2025 Size: 23 21 202 677 223 437 442 Fragments arranged by size: 677 442 437 223 202 23 21 EcoICRI GAG'CTC Cuts at: 0 33 1104 2025 Size: 33 1071 921 Fragments arranged by size: 1071 921 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 48 118 205 256 1017 1291 1618 1809 Size: 48 70 87 51 761 274 327 191 Cuts at: 1809 1884 2025 Size: 75 141 Fragments arranged by size: 761 327 274 191 141 87 75 70 51 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 556 595 2025 Size: 556 39 1430 Fragments arranged by size: 1430 556 39 EcoO109I rG'GnC_Cy Cuts at: 0 180 351 440 441 516 629 749 1041 Size: 180 171 89 1 75 113 120 292 Cuts at: 1041 1251 1318 1391 1466 1467 1872 1873 1962 Size: 210 67 73 75 1 405 1 89 Cuts at: 1962 2025 Size: 63 Fragments arranged by size: 405 292 210 180 171 120 113 89 89 75 75 73 67 63 1 1 1 EcoRI G'AATT_C Cuts at: 0 1648 2025 Size: 1648 377 HaeII r_GCGC'y Cuts at: 0 57 97 256 363 705 792 1032 1427 Size: 57 40 159 107 342 87 240 395 Cuts at: 1427 1845 2025 Size: 418 180 Fragments arranged by size: 418 395 342 240 180 159 107 87 57 40 HincII GTy'rAC Cuts at: 0 1505 2025 Size: 1505 520 KasI G'GCGC_C Cuts at: 0 53 1028 2025 Size: 53 975 997 Fragments arranged by size: 997 975 53 MscI TGG'CCA Cuts at: 0 709 1559 2025 Size: 709 850 466 Fragments arranged by size: 850 709 466 MslI CAynn'nnrTG Cuts at: 0 269 1120 1742 2025 Size: 269 851 622 283 Fragments arranged by size: 851 622 283 269 MspA1I CmG'CkG Cuts at: 0 22 213 390 1131 1137 1416 1698 1944 Size: 22 191 177 741 6 279 282 246 Cuts at: 1944 2025 Size: 81 Fragments arranged by size: 741 282 279 246 191 177 81 22 6 NaeI GCC'GGC Cuts at: 0 1191 1684 2025 Size: 1191 493 341 Fragments arranged by size: 1191 493 341 NarI GG'CG_CC Cuts at: 0 54 1029 2025 Size: 54 975 996 Fragments arranged by size: 996 975 54 NcoI C'CATG_G Cuts at: 0 1022 1095 2025 Size: 1022 73 930 Fragments arranged by size: 1022 930 73 NgoMIV G'CCGG_C Cuts at: 0 1189 1682 2025 Size: 1189 493 343 Fragments arranged by size: 1189 493 343 NspI r_CATG'y Cuts at: 0 1806 2025 Size: 1806 219 PasI CC'CwG_GG Cuts at: 0 1203 2025 Size: 1203 822 PflMI CCAn_nnn'nTGG Cuts at: 0 858 963 2025 Size: 858 105 1062 Fragments arranged by size: 1062 858 105 PfoI T'CCnGG_A Cuts at: 0 64 490 1825 2025 Size: 64 426 1335 200 Fragments arranged by size: 1335 426 200 64 PmlI CAC'GTG Cuts at: 0 1443 2025 Size: 1443 582 PpuMI rG'GwC_Cy Cuts at: 0 180 351 516 1251 2025 Size: 180 171 165 735 774 Fragments arranged by size: 774 735 180 171 165 PspOMI G'GGCC_C Cuts at: 0 6 39 440 1318 1466 1872 1962 2025 Size: 6 33 401 878 148 406 90 63 Fragments arranged by size: 878 406 401 148 90 63 33 6 PstI C_TGCA'G Cuts at: 0 908 1070 1136 2025 Size: 908 162 66 889 Fragments arranged by size: 908 889 162 66 PvuII CAG'CTG Cuts at: 0 213 1131 1137 2025 Size: 213 918 6 888 Fragments arranged by size: 918 888 213 6 SacI G_AGCT'C Cuts at: 0 35 1106 2025 Size: 35 1071 919 Fragments arranged by size: 1071 919 35 SacII CC_GC'GG Cuts at: 0 23 2025 Size: 23 2002 ScaI AGT'ACT Cuts at: 0 811 2025 Size: 811 1214 SfcI C'TryA_G Cuts at: 0 204 753 876 904 1066 1132 2025 Size: 204 549 123 28 162 66 893 Fragments arranged by size: 893 549 204 162 123 66 28 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1207 2025 Size: 1207 818 SfoI GGC'GCC Cuts at: 0 55 1030 2025 Size: 55 975 995 Fragments arranged by size: 995 975 55 SmaI CCC'GGG Cuts at: 0 38 522 1396 1870 2025 Size: 38 484 874 474 155 Fragments arranged by size: 874 484 474 155 38 SmlI C'TyrA_G Cuts at: 0 346 2025 Size: 346 1679 StuI AGG'CCT Cuts at: 0 562 871 2025 Size: 562 309 1154 Fragments arranged by size: 1154 562 309 StyI C'CwwG_G Cuts at: 0 317 1022 1095 2025 Size: 317 705 73 930 Fragments arranged by size: 930 705 317 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 667 1657 1750 2025 Size: 667 990 93 275 Fragments arranged by size: 990 667 275 93 TatI w'GTAC_w Cuts at: 0 809 2025 Size: 809 1216 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 282 314 335 367 1471 1503 2025 Size: 282 32 21 32 1104 32 522 Fragments arranged by size: 1104 522 282 32 32 32 21 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 330 2025 Size: 330 1695 XmaI C'CCGG_G Cuts at: 0 36 520 1394 1868 2025 Size: 36 484 874 474 157 Fragments arranged by size: 874 484 474 157 36 ZraI GAC'GTC Cuts at: 0 1431 2025 Size: 1431 594 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII ScaI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmrI BmtI BplI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrGI BstBI BstXI BstZ17I Bsu36I DraI DrdI EarI EciI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI