################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 577 GTAGAC GT^[AC][GT]AC AccII 305 CGCG CG^CG AocI 837 CCTCAGG CC^T[ACGT]AGG BanI 196 GGCACC G^G[CT][GA]CC BciVI 892 GGATAC GTATCC(6/5) BfaI 325 CTAG C^TAG BsaAI 480 CACGTA [CT]AC^GT[GA] BsaWI 760 ACCGGA [AT]^CCGG[AT] BseRI 212 GAGGAG GAGGAG(10/8) BsrBI 345 GAGCGG CCGCTC(-3/-3) BsrFI 377 GCCGGC [GA]^CCGG[CT] BstUI 305 CGCG CG^CG Bsu36I 837 CCTCAGG CC^T[ACGT]AGG CfoI 32 GCGC GCG^C Cfr10I 377 GCCGGC [GA]^CCGG[CT] EaeI 635 TGGCCG [CT]^GGCC[GA] Eco81I 837 CCTCAGG CC^T[ACGT]AGG EcoT14I 182 CCAAGG C^C[AT][AT]GG HaeII 31 GGCGCT [GA]GCGC^[CT] HhaI 32 GCGC GCG^C HinP1I 32 GCGC G^CGC MaeI 325 CTAG C^TAG MaeIII 398 GTGAC ^GT[ACGT]AC MboII 105 GAAGA GAAGA(8/7) MseI 755 TTAA T^TAA MspA1I 436 CAGCGG C[AC]G^C[GT]G MvnI 305 CGCG CG^CG NaeI 377 GCCGGC GCC^GGC NgoMIV 377 GCCGGC G^CCGGC PleI 846 GACTC GAGTC(4/5) PshAI 88 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 568 GAGCTC GAGCT^C SexAI 581 ACCTGGT A^CC[AT]GGT SfaNI 869 GCATC GCATC(5/9) StyI 182 CCAAGG C^C[AT][AT]GG TfiI 697 GATTC G^A[AT]TC Tru9I 755 TTAA T^TAA Tsp45I 398 GTGAC ^GT[GC]AC TspRI 687 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BcnI 157 CCGGG CC^[GC]GG 468 CCGGG BpmI 76 CTCCAG CTGGAG(16/14) 118 CTGGAG BsmFI 470 GGGAC GGGAC(10/14) 844 GGGAC BstF5I 537 CATCC GGATG(2/0) 447 GGATG DpnII 623 GATC ^GATC 751 GATC FauI 303 CCCGC CCCGC(4/6) 691 GCGGG FokI 537 CATCC GGATG(9/13) 447 GGATG HinfI 697 GATTC G^A[ACGT]TC 846 GACTC HphI 102 GGTGA GGTGA(8/7) 137 TCACC MboI 623 GATC ^GATC 751 GATC NciI 157 CCGGG CC^[GC]GG 468 CCGGG NdeII 623 GATC ^GATC 751 GATC PpuMI 23 GGGTCCT [GA]G^G[AT]CC[CT] 317 AGGACCT Sau3AI 623 GATC ^GATC 751 GATC Tsp509I 766 AATT ^AATT 898 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI ApaI AspHI AvaII BanII BbvI BglI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeII MnlI MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI TseI