(Circular) (Six-base) MAPSORT of: pFN21AE5540.seq Check: 9305 from: 1 to: 5788 pFN21AE5540 5788 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2903 3851 5235 5503 1632 Size: 1271 948 1384 268 1917 Fragments arranged by size: 1917 1384 1271 948 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 2044 2123 2237 2365 2722 2794 2989 3067 3531 Size: 79 114 128 357 72 195 78 464 Cuts at: 3531 5103 2044 Size: 1572 2729 Fragments arranged by size: 2729 1572 464 357 195 128 114 79 78 72 ApaI G_GGCC'C Cuts at: 2133 2133 Size: 5788 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 2165 3097 3323 3475 5584 1493 Size: 672 932 226 152 2109 1697 Fragments arranged by size: 2109 1697 932 672 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2388 2591 3113 1870 Size: 73 445 203 522 4545 Fragments arranged by size: 4545 522 445 203 73 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3109 4564 618 Size: 325 217 627 1322 1455 1842 Fragments arranged by size: 1842 1455 1322 627 325 217 BanII G_rGCy'C Cuts at: 1391 2133 3037 3107 1391 Size: 742 904 70 4072 Fragments arranged by size: 4072 904 742 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2631 928 Size: 971 732 4085 Fragments arranged by size: 4085 971 732 BbvCI CC'TCA_GC Cuts at: 2517 2517 Size: 5788 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2240 2513 2531 Size: 107 122 71 631 605 568 273 18 Cuts at: 2531 2593 2642 4399 136 Size: 62 49 1757 1525 Fragments arranged by size: 1757 1525 631 605 568 273 122 107 71 62 49 18 BglII A'GATC_T Cuts at: 2055 5783 2055 Size: 3728 2060 BlpI GC'TnA_GC Cuts at: 2193 2290 3205 2193 Size: 97 915 4776 Fragments arranged by size: 4776 915 97 Bme1580I G_kGCm'C Cuts at: 1790 2133 3849 5005 1790 Size: 343 1716 1156 2573 Fragments arranged by size: 2573 1716 1156 343 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2211 2744 2816 3089 4448 1239 Size: 972 533 72 273 1359 2579 Fragments arranged by size: 2579 1359 972 533 273 72 Bpu10I CC'TnA_GC Cuts at: 2517 2517 Size: 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2094 3913 4778 5076 2094 Size: 1819 865 298 2806 Fragments arranged by size: 2806 1819 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 3595 3977 4612 Size: 53 83 186 936 495 1567 382 635 Cuts at: 4612 275 Size: 1451 Fragments arranged by size: 1567 1451 936 635 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2169 2463 4221 4619 4893 5040 5451 2169 Size: 294 1758 398 274 147 411 2506 Fragments arranged by size: 2506 1758 411 398 294 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2534 2813 1186 Size: 1348 279 4161 Fragments arranged by size: 4161 1348 279 BseYI C'CCAG_C Cuts at: 4991 5487 4991 Size: 496 5292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2554 1163 Size: 573 818 4397 Fragments arranged by size: 4397 818 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2940 3238 3999 5027 1540 Size: 275 186 939 298 761 1028 2301 Fragments arranged by size: 2301 1028 939 761 298 275 186 BsiHKAI G_wGCw'C Cuts at: 2199 3849 3934 5005 2199 Size: 1650 85 1071 2982 Fragments arranged by size: 2982 1650 1071 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2199 3037 3107 3849 3934 5005 Size: 399 343 66 838 70 742 85 1071 Cuts at: 5005 1391 Size: 2174 Fragments arranged by size: 2174 1071 838 742 399 343 85 70 66 BspEI T'CCGG_A Cuts at: 2169 5451 2169 Size: 3282 2506 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2043 2997 3143 844 Size: 1199 954 146 3489 Fragments arranged by size: 3489 1199 954 146 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4283 4457 65 Size: 1183 3035 174 1396 Fragments arranged by size: 3035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2632 2712 2748 2919 2939 2973 4432 Size: 110 822 80 36 171 20 34 1459 Cuts at: 4432 4619 1700 Size: 187 2869 Fragments arranged by size: 2869 1459 822 187 171 110 80 36 34 20 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 2087 3073 1925 Size: 162 986 4640 Fragments arranged by size: 4640 986 162 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 2989 794 Size: 2195 3593 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2212 2212 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2055 2376 2847 3118 3485 3870 3887 5339 Size: 83 321 471 271 367 385 17 1452 Cuts at: 5339 5425 5437 5783 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 471 385 367 346 321 271 86 83 17 12 Bsu36I CC'TnA_GG Cuts at: 2253 2253 Size: 5788 BtgI C'CryG_G Cuts at: 513 1065 2004 2526 2579 513 Size: 552 939 522 53 3722 Fragments arranged by size: 3722 939 552 522 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2407 3304 4098 4118 2407 Size: 897 794 20 4077 Fragments arranged by size: 4077 897 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2316 2634 Size: 54 1425 50 275 18 216 270 318 Cuts at: 2634 2767 2937 3235 4124 5753 8 Size: 133 170 298 889 1629 43 Fragments arranged by size: 1629 1425 889 318 298 275 270 216 170 133 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2937 3235 1537 Size: 275 1125 298 4090 Fragments arranged by size: 4090 1125 298 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2175 2465 2657 3718 1353 Size: 822 290 192 1061 3423 Fragments arranged by size: 3423 1061 822 290 192 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2458 2849 4375 4749 4895 387 Size: 1064 138 869 391 1526 374 146 1280 Fragments arranged by size: 1526 1280 1064 869 391 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2744 2816 2903 3089 3851 4448 Size: 393 579 533 72 87 186 762 597 Cuts at: 4448 5235 5503 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 762 597 579 533 393 268 186 87 72 EcoNI CCTnn'n_nnAGG Cuts at: 2828 2870 3059 2828 Size: 42 189 5557 Fragments arranged by size: 5557 189 42 EcoO109I rG'GnC_Cy Cuts at: 2129 2130 2565 2878 3064 2129 Size: 1 435 313 186 4853 Fragments arranged by size: 4853 435 313 186 1 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 2993 4294 2993 Size: 1301 4487 HaeII r_GCGC'y Cuts at: 1678 2731 2920 2968 3022 4935 1678 Size: 1053 189 48 54 1913 2531 Fragments arranged by size: 2531 1913 1053 189 54 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 4429 4461 4503 Size: 32 135 32 743 32 2082 32 42 Cuts at: 4503 4535 1373 Size: 32 2626 Fragments arranged by size: 2626 2082 743 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4902 5086 1305 Size: 3597 184 2007 Fragments arranged by size: 3597 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 2318 10 Size: 54 1425 343 216 270 3480 Fragments arranged by size: 3480 1425 343 270 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 3746 4105 4264 518 Size: 648 2580 359 159 2042 Fragments arranged by size: 2580 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2581 2695 2719 2911 3204 3881 5029 5274 Size: 975 114 24 192 293 677 1148 245 Cuts at: 5274 1606 Size: 2120 Fragments arranged by size: 2120 1148 975 677 293 245 192 114 24 NaeI GCC'GGC Cuts at: 2634 2750 2941 2634 Size: 116 191 5481 Fragments arranged by size: 5481 191 116 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NgoMIV G'CCGG_C Cuts at: 2632 2748 2939 2632 Size: 116 191 5481 Fragments arranged by size: 5481 191 116 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 2542 1108 Size: 1434 4354 PfoI T'CCnGG_A Cuts at: 2379 2379 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3064 3064 Size: 5788 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2129 2129 Size: 5788 PstI C_TGCA'G Cuts at: 830 2040 2128 2308 2736 2874 3140 830 Size: 1210 88 180 428 138 266 3478 Fragments arranged by size: 3478 1210 428 266 180 138 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2719 2719 Size: 5788 SacII CC_GC'GG Cuts at: 2582 2582 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 2736 3140 2736 Size: 404 5384 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2036 2124 2304 2732 2870 3136 4271 Size: 219 991 88 180 428 138 266 1135 Cuts at: 4271 4952 5143 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 991 681 428 266 219 191 180 138 88 SfiI GGCCn_nnn'nGGCC Cuts at: 2531 2531 Size: 5788 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 2919 1700 Size: 1219 4569 SmaI CCC'GGG Cuts at: 1872 2390 2593 3115 1872 Size: 518 203 522 4545 Fragments arranged by size: 4545 522 518 203 SmlI C'TyrA_G Cuts at: 820 1017 1943 2109 3892 4793 5055 820 Size: 197 926 166 1783 901 262 1553 Fragments arranged by size: 1783 1553 926 901 262 197 166 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SrfI GCCC'GGGC Cuts at: 2593 2593 Size: 5788 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2251 3081 1517 Size: 734 830 4224 Fragments arranged by size: 4224 830 734 StyI C'CwwG_G Cuts at: 513 1065 2004 2022 2211 2406 2427 3227 513 Size: 552 939 18 189 195 21 800 3074 Fragments arranged by size: 3074 939 800 552 195 189 21 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5748 5780 5748 Size: 32 5756 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 2388 2591 3113 1870 Size: 518 203 522 4545 Fragments arranged by size: 4545 522 518 203 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfoI SwaI XcmI