################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AccIII AclI AflII AgeI AscI AseI AsnI Asp700I AviII BclI BfrI BglII BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EcoRI EcoRV FbaI FspI HpaI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfuI SgrAI SnaBI SpeI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 3181 GACGTC GACGT^C Acc65I 3629 GGTACC G^GTACC AccI 3184 GTCTAC GT^[AC][GT]AC AcsI 1107 GAATTT [GA]^AATT[CT] Alw44I 2805 GTGCAC G^TGCAC ApaLI 2805 GTGCAC G^TGCAC ApoI 1107 GAATTT [GA]^AATT[CT] Asp718I 3629 GGTACC G^GTACC AvrII 369 CCTAGG C^CTAGG BamHI 3319 GGATCC G^GATCC BciVI 2068 GGATAC GTATCC(6/5) BlnI 369 CCTAGG C^CTAGG BsmBI 3410 CGTCTC CGTCTC(1/5) BsmI 1003 GAATGC GAATGC(1/-1) Bsp1407I 1716 TGTACA T^GTACA BsrGI 1716 TGTACA T^GTACA EcoT22I 2586 ATGCAT ATGCA^T HincII 352 GTCAAC GT[CT]^[GA]AC HindII 352 GTCAAC GT[CT]^[GA]AC HindIII 4228 AAGCTT A^AGCTT KpnI 3629 GGTACC GGTAC^C MseI 1400 TTAA T^TAA NsiI 2586 ATGCAT ATGCA^T PaeR7I 3247 CTCGAG C^TCGAG PflMI 3524 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 1023 GCTCTTC GCTCTTC(1/4) SexAI 301 ACCTGGT A^CC[AT]GGT SfiI 3465 GGCCACAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 3247 CTCGAG C^T[CT][GA]AG SspBI 1716 TGTACA T^GTACA Tru9I 1400 TTAA T^TAA Tsp509I 1108 AATT ^AATT Van91I 3524 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 711 TCTAGA T^CTAGA XhoI 3247 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflIII 155 ACACGT A^C[GA][CT]GT 725 ACACGT AhdI 1150 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2473 GACCCCCTGTC AspEI 1150 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2473 GACCCCCTGTC AspI 4087 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4449 GACCTTGTC BbeI 89 GGCGCC GGCGC^C 196 GGCGCC BbrPI 156 CACGTG CAC^GTG 190 CACGTG BcgI 4704 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2225 GCAACCCTTTCG BlpI 4369 GCTGAGC GC^T[ACGT]AGC 4389 GCTGAGC Bpu1102I 4369 GCTGAGC GC^T[ACGT]AGC 4389 GCTGAGC BsiEI 50 CGGCCG CG[GA][CT]^CG 513 CGGCCG BstEII 684 GGTCACC G^GT[ACGT]ACC 2518 GGTGACC BstPI 684 GGTCACC G^GT[ACGT]ACC 2518 GGTGACC CelII 4369 GCTGAGC GC^T[ACGT]AGC 4389 GCTGAGC DraIII 300 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1085 CACACTGTG EagI 50 CGGCCG C^GGCCG 513 CGGCCG Eam1105I 1150 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2473 GACCCCCTGTC EarI 1024 CTCTTC CTCTTC(1/4) 3376 GAAGAG EclXI 50 CGGCCG C^GGCCG 513 CGGCCG Eco52I 50 CGGCCG C^GGCCG 513 CGGCCG EcoO65I 684 GGTCACC G^GT[ACGT]ACC 2518 GGTGACC FseI 514 GGCCGGCC GGCCGG^CC 634 GGCCGGCC KasI 89 GGCGCC G^GCGCC 196 GGCGCC Ksp632I 1024 CTCTTC CTCTTC(1/4) 3376 GAAGAG NarI 89 GGCGCC GG^CGCC 196 GGCGCC NcoI 320 CCATGG C^CATGG 466 CCATGG PflFI 4087 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4449 GACCTTGTC PmaCI 156 CACGTG CAC^GTG 190 CACGTG PmlI 156 CACGTG CAC^GTG 190 CACGTG SbfI 1824 CCTGCAGG CCTGCA^GG 2891 CCTGCAGG SfoI 89 GGCGCC GGC^GCC 196 GGCGCC Sse8387I 1824 CCTGCAGG CCTGCA^GG 2891 CCTGCAGG Tth111I 4087 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4449 GACCTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SphI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI