(Circular) (Six-base) MAPSORT of: pFN21AE6736.seq Check: 8799 from: 1 to: 9430 pFN21AE6736 9430 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2802 3212 3837 4823 4890 5214 6322 2802 Size: 410 625 986 67 324 1108 5910 Fragments arranged by size: 5910 1108 986 625 410 324 67 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5190 7240 278 Size: 53 83 186 936 3654 2050 2468 Fragments arranged by size: 3654 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5190 6773 1937 Size: 3253 1583 4594 Fragments arranged by size: 4594 3253 1583 Acc65I G'GTAC_C Cuts at: 5634 6751 5634 Size: 1117 8313 AclI AA'CG_TT Cuts at: 1563 7557 7930 1563 Size: 5994 373 3063 Fragments arranged by size: 5994 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 5105 5715 5820 5834 6366 7493 8877 Size: 797 2676 610 105 14 532 1127 1384 Cuts at: 8877 9145 1632 Size: 268 1917 Fragments arranged by size: 2676 1917 1384 1127 797 610 532 268 105 14 AfeI AGC'GCT Cuts at: 2105 2231 2912 3101 2105 Size: 126 681 189 8434 Fragments arranged by size: 8434 681 189 126 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9233 AflIII A'CryG_T Cuts at: 1203 2160 2730 8329 9306 1203 Size: 957 570 5599 977 1327 Fragments arranged by size: 5599 1327 977 957 570 AgeI A'CCGG_T Cuts at: 8261 8261 Size: 9430 AhdI GACnn_n'nnGTC Cuts at: 3160 4483 8159 3160 Size: 1323 3676 4431 Fragments arranged by size: 4431 3676 1323 AleI CACnn'nnGTG Cuts at: 1166 2399 3069 4952 6276 1166 Size: 1233 670 1883 1324 4320 Fragments arranged by size: 4320 1883 1324 1233 670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4606 4711 5418 6294 1184 Size: 3422 105 707 876 4320 Fragments arranged by size: 4320 3422 876 707 105 AlwNI CAG_nnn'CTG Cuts at: 2539 3291 3441 4704 5142 5293 5531 5996 6337 Size: 752 150 1263 438 151 238 465 341 Cuts at: 6337 6364 6517 6625 6682 7173 8745 2539 Size: 27 153 108 57 491 1572 3224 Fragments arranged by size: 3224 1572 1263 752 491 465 438 341 238 153 151 150 108 57 27 ApaI G_GGCC'C Cuts at: 2947 4196 5545 2947 Size: 1249 1349 6832 Fragments arranged by size: 6832 1349 1249 ApaLI G'TGCA_C Cuts at: 4810 7487 8643 4810 Size: 2677 1156 5597 Fragments arranged by size: 5597 2677 1156 ApoI r'AATT_y Cuts at: 1493 3112 6739 6965 7117 9226 1493 Size: 1619 3627 226 152 2109 1697 Fragments arranged by size: 3627 2109 1697 1619 226 152 AseI AT'TA_AT Cuts at: 160 1032 7984 160 Size: 872 6952 1606 Fragments arranged by size: 6952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9430 AvaI C'yCGr_G Cuts at: 1870 1943 2177 2525 2946 3254 3394 3701 4339 Size: 73 234 348 421 308 140 307 638 Cuts at: 4339 5220 5252 5274 5770 5793 6568 6707 6755 Size: 881 32 22 496 23 775 139 48 Cuts at: 6755 1870 Size: 4545 Fragments arranged by size: 4545 881 775 638 496 421 348 308 307 234 140 139 73 48 32 23 22 AvrII C'CTAG_G Cuts at: 2374 2374 Size: 9430 BamHI G'GATC_C Cuts at: 5324 6760 7127 5324 Size: 1436 367 7627 Fragments arranged by size: 7627 1436 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2094 2201 2877 2951 Size: 325 217 627 257 50 107 676 74 Cuts at: 2951 3200 3694 4142 4410 4851 5303 5634 6751 Size: 249 494 448 268 441 452 331 1117 Cuts at: 6751 8206 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1117 676 627 494 452 448 441 331 325 268 257 249 217 107 74 50 BanII G_rGCy'C Cuts at: 1391 2221 2321 2481 2672 2947 3030 3560 3584 Size: 830 100 160 191 275 83 530 24 Cuts at: 3584 3605 3624 3819 4196 4404 4622 4775 5221 Size: 21 19 195 377 208 218 153 446 Cuts at: 5221 5545 5779 6566 6749 1391 Size: 324 234 787 183 4072 Fragments arranged by size: 4072 830 787 530 446 377 324 275 234 218 208 195 191 183 160 153 100 83 24 21 19 BbeI G_GCGC'C Cuts at: 2098 2205 2098 Size: 107 9323 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2516 3615 5339 5808 5840 6137 928 Size: 971 617 1099 1724 469 32 297 4221 Fragments arranged by size: 4221 1724 1099 971 617 469 297 32 BbvCI CC'TCA_GC Cuts at: 3506 3728 6251 3506 Size: 222 2523 6685 Fragments arranged by size: 6685 2523 222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4219 4253 6698 6732 7621 7655 1903 Size: 34 2282 34 2445 34 889 34 3678 Fragments arranged by size: 3678 2445 2282 889 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 7322 8532 4067 Size: 3255 1210 4965 Fragments arranged by size: 4965 3255 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9430 BfrBI ATG'CAT Cuts at: 4593 4593 Size: 9430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3780 4341 Size: 107 122 71 631 605 337 1771 561 Cuts at: 4341 5477 5762 5867 8041 136 Size: 1136 285 105 2174 1525 Fragments arranged by size: 2174 1771 1525 1136 631 605 561 337 285 122 107 105 71 BglII A'GATC_T Cuts at: 9425 9425 Size: 9430 BlpI GC'TnA_GC Cuts at: 6375 6395 6847 6375 Size: 20 452 8958 Fragments arranged by size: 8958 452 20 Bme1580I G_kGCm'C Cuts at: 1790 2947 2954 3699 4196 4413 4814 5308 5317 Size: 1157 7 745 497 217 401 494 9 Cuts at: 5317 5488 5545 6538 7491 8647 1790 Size: 171 57 993 953 1156 2573 Fragments arranged by size: 2573 1157 1156 993 953 745 497 494 401 217 171 57 9 7 BmgBI CAC'GTC Cuts at: 1318 2854 3850 1318 Size: 1536 996 6898 Fragments arranged by size: 6898 1536 996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3133 3478 4643 Size: 416 53 83 399 24 1716 345 1165 Cuts at: 4643 4778 4922 4924 5436 7161 8119 442 Size: 135 144 2 512 1725 958 1753 Fragments arranged by size: 1753 1725 1716 1165 958 512 416 399 345 144 135 83 53 24 2 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 2753 3167 4712 5255 5378 5390 5441 Size: 1415 99 414 1545 543 123 12 51 Cuts at: 5441 5609 5645 5849 5867 6163 6386 6458 6620 Size: 168 36 204 18 296 223 72 162 Cuts at: 6620 6704 8090 1239 Size: 84 1386 2579 Fragments arranged by size: 2579 1545 1415 1386 543 414 296 223 204 168 162 123 99 84 72 51 36 18 12 Bpu10I CC'TnA_GC Cuts at: 3506 3599 3728 3818 6251 3506 Size: 93 129 90 2433 6685 Fragments arranged by size: 6685 2433 129 93 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7555 8420 8718 7555 Size: 865 298 8267 Fragments arranged by size: 8267 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3642 3830 5603 6446 8093 882 Size: 617 2143 188 1773 843 1647 2219 Fragments arranged by size: 2219 2143 1773 1647 843 617 188 BsaAI yAC'GTr Cuts at: 493 1204 2163 2197 2397 7221 493 Size: 711 959 34 200 4824 2702 Fragments arranged by size: 4824 2702 959 711 200 34 BsaBI GATnn'nnATC Cuts at: 9424 9424 Size: 9430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2095 2202 2238 5187 Size: 53 83 186 936 562 107 36 2949 Cuts at: 5187 6323 6341 7237 7619 8254 275 Size: 1136 18 896 382 635 1451 Fragments arranged by size: 2949 1451 1136 936 896 635 562 382 186 107 83 53 36 18 BsaWI w'CCGG_w Cuts at: 7863 8261 8535 8682 9093 7863 Size: 398 274 147 411 8200 Fragments arranged by size: 8200 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2252 2282 2483 2513 4307 Size: 30 165 30 849 30 201 30 1794 Cuts at: 4307 4337 4772 4802 5618 5648 1178 Size: 30 435 30 816 30 4960 Fragments arranged by size: 4960 1794 849 816 435 201 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2678 2696 3629 3632 3878 5084 5399 1186 Size: 1492 18 933 3 246 1206 315 5217 Fragments arranged by size: 5217 1492 1206 933 315 246 18 3 BseYI C'CCAG_C Cuts at: 2026 2250 2645 3083 3932 4396 4416 4858 5164 Size: 224 395 438 849 464 20 442 306 Cuts at: 5164 5343 5613 6154 6327 6389 6478 6505 6559 Size: 179 270 541 173 62 89 27 54 Cuts at: 6559 6612 8633 9129 2026 Size: 53 2021 496 2327 Fragments arranged by size: 2327 2021 849 541 496 464 442 438 395 306 270 224 179 173 89 62 54 53 27 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2360 2557 3277 4108 4493 4671 4978 Size: 573 624 197 720 831 385 178 307 Cuts at: 4978 5323 5741 5918 6680 1163 Size: 345 418 177 762 3913 Fragments arranged by size: 3913 831 762 720 624 573 418 385 345 307 197 178 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2521 6880 7641 8669 1540 Size: 275 186 57 463 4359 761 1028 2301 Fragments arranged by size: 4359 2301 1028 761 463 275 186 57 BsiHKAI G_wGCw'C Cuts at: 2306 2769 3030 3567 3624 3819 4069 4539 4814 Size: 463 261 537 57 195 250 470 275 Cuts at: 4814 4907 5779 7491 7576 8647 2306 Size: 93 872 1712 85 1071 3089 Fragments arranged by size: 3089 1712 1071 872 537 470 463 275 261 250 195 93 85 57 BsmI GAATG_Cn' Cuts at: 3014 6941 7034 3014 Size: 3927 93 5410 Fragments arranged by size: 5410 3927 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5421 5421 Size: 9430 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2221 2306 2321 2481 2672 2769 2947 Size: 399 431 85 15 160 191 97 178 Cuts at: 2947 2954 3030 3560 3567 3584 3605 3624 3699 Size: 7 76 530 7 17 21 19 75 Cuts at: 3699 3819 4069 4196 4404 4413 4539 4622 4775 Size: 120 250 127 208 9 126 83 153 Cuts at: 4775 4814 4907 5221 5308 5317 5488 5545 5779 Size: 39 93 314 87 9 171 57 234 Cuts at: 5779 6538 6566 6749 7491 7576 8647 1391 Size: 759 28 183 742 85 1071 2174 Fragments arranged by size: 2174 1071 759 742 530 431 399 314 250 234 208 191 183 178 171 160 153 127 126 120 97 93 87 85 85 83 76 75 57 39 28 21 19 17 15 9 9 7 7 BspEI T'CCGG_A Cuts at: 9093 9093 Size: 9430 BspHI T'CATG_A Cuts at: 7319 9049 7319 Size: 1730 7700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2547 2802 3212 3417 3837 4823 4890 5214 Size: 1703 255 410 205 420 986 67 324 Cuts at: 5214 5345 6322 6718 6785 844 Size: 131 977 396 67 3489 Fragments arranged by size: 3489 1703 986 977 420 410 396 324 255 205 131 67 67 BsrBI CCG'CTC Cuts at: 1394 1823 3000 6259 6379 7317 1394 Size: 429 1177 3259 120 938 3507 Fragments arranged by size: 3507 3259 1177 938 429 120 BsrDI GCAATG_nn' Cuts at: 65 1248 2221 5045 6074 7925 8099 65 Size: 1183 973 2824 1029 1851 174 1396 Fragments arranged by size: 2824 1851 1396 1183 1029 973 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2057 2113 2520 2640 2721 2874 2894 Size: 110 247 56 407 120 81 153 20 Cuts at: 2894 3941 8074 8261 1700 Size: 1047 4133 187 2869 Fragments arranged by size: 4133 2869 1047 407 247 187 153 120 110 81 56 20 BsrGI T'GTAC_A Cuts at: 96 3721 96 Size: 3625 5805 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9430 BssSI C'ACGA_G Cuts at: 2969 4937 6121 7490 8502 2969 Size: 1968 1184 1369 1012 3897 Fragments arranged by size: 3897 1968 1369 1184 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3291 4599 4704 5031 5531 6625 6703 794 Size: 2497 1308 105 327 500 1094 78 3521 Fragments arranged by size: 3521 2497 1308 1094 500 327 105 78 BstBI TT'CG_AA Cuts at: 8234 8234 Size: 9430 BstEII G'GTnAC_C Cuts at: 1184 2689 4523 1184 Size: 1505 1834 6091 Fragments arranged by size: 6091 1834 1505 BstXI CCAn_nnnn'nTGG Cuts at: 3083 3550 4008 4465 3083 Size: 467 458 457 8048 Fragments arranged by size: 8048 467 458 457 BstYI r'GATC_y Cuts at: 1972 4164 5324 6414 6630 6660 6760 7127 7512 Size: 2192 1160 1090 216 30 100 367 385 Cuts at: 7512 7529 8981 9067 9079 9425 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2192 1977 1452 1160 1090 385 367 346 216 100 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3761 5430 6548 3761 Size: 1669 1118 6643 Fragments arranged by size: 6643 1669 1118 BtgI C'CryG_G Cuts at: 513 1065 2004 2048 2205 2325 2471 2939 3046 Size: 552 939 44 157 120 146 468 107 Cuts at: 3046 3131 3219 4464 4518 513 Size: 85 88 1245 54 5425 Fragments arranged by size: 5425 1245 939 552 468 157 146 120 107 88 85 54 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3204 487 Size: 683 56 1978 6713 Fragments arranged by size: 6713 1978 683 56 BtsI GCAGTG_nn' Cuts at: 2998 3064 3343 3477 3860 4330 4492 5029 5370 Size: 66 279 134 383 470 162 537 341 Cuts at: 5370 5529 6946 7740 7760 2998 Size: 159 1417 794 20 4668 Fragments arranged by size: 4668 1417 794 537 470 383 341 279 162 159 134 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2974 3009 529 Size: 35 2410 35 6950 Fragments arranged by size: 6950 2410 35 35 DraI TTT'AAA Cuts at: 6734 6734 Size: 9430 DraIII CAC_nnn'GTG Cuts at: 2310 3095 2310 Size: 785 8645 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8437 809 Size: 507 7121 1802 Fragments arranged by size: 7121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2518 2995 Size: 54 1425 50 275 18 225 463 477 Cuts at: 2995 3454 3983 4279 4489 5469 5712 5859 6216 Size: 459 529 296 210 980 243 147 357 Cuts at: 6216 6877 7766 9395 8 Size: 661 889 1629 43 Fragments arranged by size: 1629 1425 980 889 661 529 477 463 459 357 296 275 243 225 210 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2055 2518 6877 1537 Size: 275 243 463 4359 4090 Fragments arranged by size: 4359 4090 463 275 243 EarI CTCTTCn'nnn_ Cuts at: 1353 3035 5376 7360 1353 Size: 1682 2341 1984 3423 Fragments arranged by size: 3423 2341 1984 1682 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2360 3899 3967 6158 8017 8391 Size: 1064 138 771 1539 68 2191 1859 374 Cuts at: 8391 8537 387 Size: 146 1280 Fragments arranged by size: 2191 1859 1539 1280 1064 771 374 146 138 68 EcoICRI GAG'CTC Cuts at: 3028 3622 3817 5777 3028 Size: 594 195 1960 6681 Fragments arranged by size: 6681 1960 594 195 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2654 2753 3167 4712 5105 5255 Size: 393 797 225 99 414 1545 393 150 Cuts at: 5255 5378 5390 5441 5609 5645 5715 5820 5834 Size: 123 12 51 168 36 70 105 14 Cuts at: 5834 5849 5867 6163 6366 6386 6458 6620 6704 Size: 15 18 296 203 20 72 162 84 Cuts at: 6704 7493 8090 8877 9145 1239 Size: 789 597 787 268 1524 Fragments arranged by size: 1545 1524 797 789 787 597 414 393 393 296 268 225 203 168 162 150 123 105 99 84 72 70 51 36 20 18 15 14 12 EcoNI CCTnn'n_nnAGG Cuts at: 2378 3959 4846 5063 5433 5960 6250 6580 2378 Size: 1581 887 217 370 527 290 330 5228 Fragments arranged by size: 5228 1581 887 527 370 330 290 217 EcoO109I rG'GnC_Cy Cuts at: 2755 3127 3399 3592 4100 4192 4390 5173 5438 Size: 372 272 193 508 92 198 783 265 Cuts at: 5438 5541 5542 5620 5824 6514 6573 2755 Size: 103 1 78 204 690 59 5612 Fragments arranged by size: 5612 783 690 508 372 272 265 204 198 193 103 92 78 59 1 EcoRI G'AATT_C Cuts at: 6739 7117 6739 Size: 378 9052 FseI GG_CCGG'CC Cuts at: 2524 2644 2524 Size: 120 9310 FspI TGC'GCA Cuts at: 7936 7936 Size: 9430 HaeII r_GCGC'y Cuts at: 1678 2039 2098 2107 2205 2233 2257 2499 2652 Size: 361 59 9 98 28 24 242 153 Cuts at: 2652 2914 3058 3103 3346 4360 4566 4920 8577 Size: 262 144 45 243 1014 206 354 3657 Cuts at: 8577 1678 Size: 2531 Fragments arranged by size: 3657 2531 1014 361 354 262 243 242 206 153 144 98 59 45 28 24 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3146 3178 4181 4213 4469 Size: 32 135 32 1574 32 1003 32 256 Cuts at: 4469 4501 6443 6475 6593 6625 8071 8103 8145 Size: 32 1942 32 118 32 1446 32 42 Cuts at: 8145 8177 1373 Size: 32 2626 Fragments arranged by size: 2626 1942 1574 1446 1003 256 135 118 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2359 6774 7020 669 Size: 988 281 421 4415 246 3079 Fragments arranged by size: 4415 3079 988 421 281 246 HindIII A'AGCT_T Cuts at: 748 6233 748 Size: 5485 3945 HpaI GTT'AAC Cuts at: 7020 7020 Size: 9430 KasI G'GCGC_C Cuts at: 2094 2201 2094 Size: 107 9323 KpnI G_GTAC'C Cuts at: 5638 6755 5638 Size: 1117 8313 MfeI C'AATT_G Cuts at: 7029 7029 Size: 9430 MluI A'CGCG_T Cuts at: 9306 9306 Size: 9430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2817 5151 5580 6340 8544 8728 1305 Size: 1512 2334 429 760 2204 184 2007 Fragments arranged by size: 2334 2204 2007 1512 760 429 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3456 4281 4491 5471 5714 Size: 54 1425 343 1624 825 210 980 243 Cuts at: 5714 5861 6218 10 Size: 147 357 3222 Fragments arranged by size: 3222 1624 1425 980 825 357 343 243 210 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 3069 4952 4954 6276 7388 7747 Size: 648 1233 670 1883 2 1322 1112 359 Cuts at: 7747 7906 518 Size: 159 2042 Fragments arranged by size: 2042 1883 1322 1233 1112 670 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2050 2207 2602 3083 3133 3246 3308 3428 Size: 444 157 395 481 50 113 62 120 Cuts at: 3428 3441 3655 3727 3883 4113 4396 4605 4704 Size: 13 214 72 156 230 283 209 99 Cuts at: 4704 4858 4992 5284 5310 5347 5402 5617 6559 Size: 154 134 292 26 37 55 215 942 Cuts at: 6559 6723 6846 7523 8671 8916 1606 Size: 164 123 677 1148 245 2120 Fragments arranged by size: 2120 1148 942 677 481 444 395 292 283 245 230 215 214 209 164 157 156 154 134 123 120 113 99 72 62 55 50 37 26 13 NaeI GCC'GGC Cuts at: 2059 2522 2642 2059 Size: 463 120 8847 Fragments arranged by size: 8847 463 120 NarI GG'CG_CC Cuts at: 2095 2202 2095 Size: 107 9323 NcoI C'CATG_G Cuts at: 513 1065 2004 2325 2471 513 Size: 552 939 321 146 7472 Fragments arranged by size: 7472 939 552 321 146 NdeI CA'TA_TG Cuts at: 387 387 Size: 9430 NgoMIV G'CCGG_C Cuts at: 2057 2520 2640 2057 Size: 463 120 8847 Fragments arranged by size: 8847 463 120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9430 NotI GC'GGCC_GC Cuts at: 6877 6877 Size: 9430 NsiI A_TGCA'T Cuts at: 4595 4595 Size: 9430 NspI r_CATG'y Cuts at: 1132 1229 2834 4597 4953 5399 6788 8333 1132 Size: 97 1605 1763 356 446 1389 1545 2229 Fragments arranged by size: 2229 1763 1605 1545 1389 446 356 97 PasI CC'CwG_GG Cuts at: 1348 2672 3804 5142 1348 Size: 1324 1132 1338 5636 Fragments arranged by size: 5636 1338 1324 1132 PciI A'CATG_T Cuts at: 8329 8329 Size: 9430 PflMI CCAn_nnn'nTGG Cuts at: 1108 5535 1108 Size: 4427 5003 PfoI T'CCnGG_A Cuts at: 2928 4069 5232 6417 2928 Size: 1141 1163 1185 5941 Fragments arranged by size: 5941 1185 1163 1141 PmeI GTTT'AAAC Cuts at: 6734 6734 Size: 9430 PmlI CAC'GTG Cuts at: 2163 2197 2163 Size: 34 9396 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 7499 7531 9012 9044 3806 Size: 32 3661 32 1481 32 4192 Fragments arranged by size: 4192 3661 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2755 3127 3399 3592 4100 4390 5438 5620 6573 Size: 372 272 193 508 290 1048 182 953 Cuts at: 6573 2755 Size: 5612 Fragments arranged by size: 5612 1048 953 508 372 290 272 193 182 PshAI GACnn'nnGTC Cuts at: 1613 2187 3246 3387 3962 1613 Size: 574 1059 141 575 7081 Fragments arranged by size: 7081 1059 575 574 141 PsiI TTA'TAA Cuts at: 7000 7000 Size: 9430 PspOMI G'GGCC_C Cuts at: 2943 4192 5541 2943 Size: 1249 1349 6832 Fragments arranged by size: 6832 1349 1249 PspXI vC'TCGA_Gb Cuts at: 5252 5252 Size: 9430 PstI C_TGCA'G Cuts at: 830 2682 2993 3287 3414 3437 3834 4178 4700 Size: 1852 311 294 127 23 397 344 522 Cuts at: 4700 4901 5289 6048 6558 6630 6782 830 Size: 201 388 759 510 72 152 3478 Fragments arranged by size: 3478 1852 759 522 510 397 388 344 311 294 201 152 127 72 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9430 PvuII CAG'CTG Cuts at: 3083 3428 3441 3727 4113 4605 4858 5284 5347 Size: 345 13 286 386 492 253 426 63 Cuts at: 5347 5402 6559 6723 3083 Size: 55 1157 164 5790 Fragments arranged by size: 5790 1157 492 426 386 345 286 253 164 63 55 13 SacI G_AGCT'C Cuts at: 3030 3624 3819 5779 3030 Size: 594 195 1960 6681 Fragments arranged by size: 6681 1960 594 195 SacII CC_GC'GG Cuts at: 2051 2208 3134 2051 Size: 157 926 8347 Fragments arranged by size: 8347 926 157 SalI G'TCGA_C Cuts at: 1936 6772 1936 Size: 4836 4594 SanDI GG'GwC_CC Cuts at: 3127 4100 4390 3127 Size: 973 290 8167 Fragments arranged by size: 8167 973 290 SapI GCTCTTCn'nnn_ Cuts at: 3035 3035 Size: 9430 SbfI CC_TGCA'GG Cuts at: 3834 4901 6782 3834 Size: 1067 1881 6482 Fragments arranged by size: 6482 1881 1067 ScaI AGT'ACT Cuts at: 7678 9390 7678 Size: 1712 7718 SexAI A'CCwGG_T Cuts at: 2306 2306 Size: 9430 SfcI C'TryA_G Cuts at: 826 1045 2491 2678 2989 3283 3410 3433 3830 Size: 219 1446 187 311 294 127 23 397 Cuts at: 3830 4174 4696 4897 5285 6044 6554 6626 6778 Size: 344 522 201 388 759 510 72 152 Cuts at: 6778 7913 8594 8785 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1446 1135 759 681 522 510 397 388 344 311 294 219 201 191 187 152 127 72 23 SfiI GGCCn_nnn'nGGCC Cuts at: 5477 5477 Size: 9430 SfoI GGC'GCC Cuts at: 2096 2203 2096 Size: 107 9323 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9430 SmaI CCC'GGG Cuts at: 1872 2948 3256 3396 3703 4341 5276 5772 5795 Size: 1076 308 140 307 638 935 496 23 Cuts at: 5795 6570 6757 1872 Size: 775 187 4545 Fragments arranged by size: 4545 1076 935 775 638 496 308 307 187 140 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 5252 7534 8435 8697 820 Size: 197 926 3309 2282 901 262 1553 Fragments arranged by size: 3309 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7221 493 Size: 6728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9430 SphI G_CATG'C Cuts at: 1132 2834 4597 5399 6788 1132 Size: 1702 1763 802 1389 3774 Fragments arranged by size: 3774 1763 1702 1389 802 SspI AAT'ATT Cuts at: 5 52 7354 5 Size: 47 7302 2081 Fragments arranged by size: 7302 2081 47 StuI AGG'CCT Cuts at: 1517 2153 4841 4963 6245 1517 Size: 636 2688 122 1282 4702 Fragments arranged by size: 4702 2688 1282 636 122 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 2374 2471 2760 3462 5119 Size: 552 939 321 49 97 289 702 1657 Cuts at: 5119 5177 6060 6219 6240 6869 513 Size: 58 883 159 21 629 3074 Fragments arranged by size: 3074 1657 939 883 702 629 552 321 289 159 97 58 49 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2344 4000 4612 4626 4779 5357 6374 Size: 1255 764 1656 612 14 153 578 1017 Cuts at: 6374 6509 7457 7474 7627 7812 8257 325 Size: 135 948 17 153 185 445 1498 Fragments arranged by size: 1656 1498 1255 1017 948 764 612 578 445 185 153 153 135 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3721 7676 9388 Size: 274 80 33 51 1443 1744 3955 1712 Cuts at: 9388 96 Size: 138 Fragments arranged by size: 3955 1744 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2252 2284 2924 2956 3286 3318 4307 4339 4772 Size: 32 640 32 330 32 989 32 433 Cuts at: 4772 4804 9390 9422 2252 Size: 32 4586 32 2260 Fragments arranged by size: 4586 2260 989 640 433 330 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6095 6457 1316 Size: 4779 362 4289 Fragments arranged by size: 4779 4289 362 XbaI T'CTAG_A Cuts at: 2716 6766 2716 Size: 4050 5380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3425 4799 6016 3425 Size: 1374 1217 6839 Fragments arranged by size: 6839 1374 1217 XhoI C'TCGA_G Cuts at: 1943 5252 1943 Size: 3309 6121 XmaI C'CCGG_G Cuts at: 1870 2946 3254 3394 3701 4339 5274 5770 5793 Size: 1076 308 140 307 638 935 496 23 Cuts at: 5793 6568 6755 1870 Size: 775 187 4545 Fragments arranged by size: 4545 1076 935 775 638 496 308 307 187 140 23 XmnI GAAnn'nnTTC Cuts at: 7559 7559 Size: 9430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5188 7238 276 Size: 53 83 186 936 3654 2050 2468 Fragments arranged by size: 3654 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BplI BsiWI BstZ17I EcoRV FalI FspAI NruI PacI PsrI RsrII SrfI SwaI