(Circular) (Six-base) MAPSORT of: pFN21AE1790.seq Check: 7467 from: 1 to: 5596 pFN21AE1790 5596 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2708 2708 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3659 5043 5311 1632 Size: 2027 1384 268 1917 Fragments arranged by size: 2027 1917 1384 268 AfeI AGC'GCT Cuts at: 2789 2789 Size: 5596 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 2370 2475 4495 5472 1203 Size: 1167 105 2020 977 1327 Fragments arranged by size: 2020 1327 1167 977 105 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 2744 1166 Size: 1578 4018 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2462 2494 2462 Size: 32 5564 AlwNI CAG_nnn'CTG Cuts at: 2624 3339 4911 2624 Size: 715 1572 3309 Fragments arranged by size: 3309 1572 715 ApaLI G'TGCA_C Cuts at: 2366 3653 4809 2366 Size: 1287 1156 3153 Fragments arranged by size: 3153 1287 1156 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2168 2185 2306 2921 1870 Size: 73 225 17 121 615 4545 Fragments arranged by size: 4545 615 225 121 73 17 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 2506 2797 2917 4372 Size: 325 217 627 314 405 291 120 1455 Cuts at: 4372 618 Size: 1842 Fragments arranged by size: 1842 1455 627 405 325 314 291 217 120 BanII G_rGCy'C Cuts at: 1391 2736 2915 1391 Size: 1345 179 4072 Fragments arranged by size: 4072 1345 179 BbeI G_GCGC'C Cuts at: 2105 2105 Size: 5596 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2395 2429 3787 3821 1903 Size: 34 50 34 374 34 1358 34 3678 Fragments arranged by size: 3678 1358 374 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2882 4207 Size: 107 122 71 631 605 337 873 1325 Cuts at: 4207 136 Size: 1525 Fragments arranged by size: 1525 1325 873 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2169 2370 2511 2682 2802 3657 4813 1790 Size: 379 201 141 171 120 855 1156 2573 Fragments arranged by size: 2573 1156 855 379 201 171 141 120 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2377 2377 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2473 2561 2729 2804 4256 1239 Size: 1234 88 168 75 1452 2579 Fragments arranged by size: 2579 1452 1234 168 88 75 Bpu10I CC'TnA_GC Cuts at: 2486 2486 Size: 5596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 2855 3721 4586 4884 2213 Size: 642 866 865 298 2925 Fragments arranged by size: 2925 866 865 642 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2248 2641 2671 2746 3387 493 Size: 711 1044 393 30 75 641 2702 Fragments arranged by size: 2702 1044 711 641 393 75 30 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2102 3403 3785 4420 Size: 53 83 186 936 569 1301 382 635 Cuts at: 4420 275 Size: 1451 Fragments arranged by size: 1451 1301 936 635 569 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5596 BseYI C'CCAG_C Cuts at: 2050 2113 4799 5295 2050 Size: 63 2686 496 2351 Fragments arranged by size: 2686 2351 496 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2575 1163 Size: 573 839 4184 Fragments arranged by size: 4184 839 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2332 2419 3046 3807 4835 Size: 275 186 60 271 87 627 761 1028 Cuts at: 4835 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 627 275 271 186 87 60 BsiHKAI G_wGCw'C Cuts at: 2370 2736 3657 3742 4813 2370 Size: 366 921 85 1071 3153 Fragments arranged by size: 3153 1071 921 366 85 BsiWI C'GTAC_G Cuts at: 2244 2244 Size: 5596 BsmI GAATG_Cn' Cuts at: 2530 3107 3200 2530 Size: 577 93 4926 Fragments arranged by size: 4926 577 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2318 2318 Size: 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2370 2511 2682 2736 2802 2915 Size: 399 379 201 141 171 54 66 113 Cuts at: 2915 3657 3742 4813 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 201 171 141 113 85 66 54 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2708 2951 844 Size: 1864 243 3489 Fragments arranged by size: 3489 1864 243 BsrBI CCG'CTC Cuts at: 1394 1823 2818 3483 1394 Size: 429 995 665 3507 Fragments arranged by size: 3507 995 665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2021 2118 2331 4240 4427 1700 Size: 110 211 97 213 1909 187 2869 Fragments arranged by size: 2869 1909 213 211 187 110 97 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 2277 1925 Size: 352 5244 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 2423 1184 Size: 1239 4357 BstXI CCAn_nnnn'nTGG Cuts at: 2782 2782 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591 Size: 954 367 385 17 1452 86 12 346 Cuts at: 5591 1972 Size: 1977 Fragments arranged by size: 1977 1452 954 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2136 2179 2272 2317 2401 2419 2466 Size: 552 1071 43 93 45 84 18 47 Cuts at: 2466 513 Size: 3643 Fragments arranged by size: 3643 1071 552 93 84 47 45 43 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2413 2682 3112 3906 3926 2413 Size: 269 430 794 20 4083 Fragments arranged by size: 4083 794 430 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2310 4603 809 Size: 507 994 2293 1802 Fragments arranged by size: 2293 1802 994 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2196 2430 3043 Size: 54 1425 50 275 18 366 234 613 Cuts at: 3043 3932 5561 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 613 366 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2609 3526 1353 Size: 1256 917 3423 Fragments arranged by size: 3423 1256 917 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2734 2734 Size: 5596 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2473 2561 2729 2804 3659 4256 5043 Size: 393 841 88 168 75 855 597 787 Cuts at: 5043 5311 1239 Size: 268 1524 Fragments arranged by size: 1524 855 841 787 597 393 268 168 88 75 EcoO109I rG'GnC_Cy Cuts at: 2174 2174 Size: 5596 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FseI GG_CCGG'CC Cuts at: 2025 2025 Size: 5596 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2015 2105 2791 4743 1678 Size: 337 90 686 1952 2531 Fragments arranged by size: 2531 1952 686 337 90 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 4237 4269 4311 Size: 32 135 32 1046 32 1587 32 42 Cuts at: 4311 4343 1373 Size: 32 2626 Fragments arranged by size: 2626 1587 1046 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KasI G'GCGC_C Cuts at: 2101 2101 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 2370 5472 2370 Size: 3102 2494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2198 10 Size: 54 1425 343 366 3408 Fragments arranged by size: 3408 1425 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3554 3913 4072 518 Size: 648 1578 810 359 159 2042 Fragments arranged by size: 2042 1578 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2181 2274 2299 2319 2403 2421 2451 3012 Size: 575 93 25 20 84 18 30 561 Cuts at: 3012 3689 4837 5082 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 575 561 245 93 84 30 25 20 18 NaeI GCC'GGC Cuts at: 2023 2120 2023 Size: 97 5499 NarI GG'CG_CC Cuts at: 2102 2102 Size: 5596 NcoI C'CATG_G Cuts at: 513 1065 2466 513 Size: 552 1401 3643 Fragments arranged by size: 3643 1401 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NgoMIV G'CCGG_C Cuts at: 2021 2118 2021 Size: 97 5499 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2954 4499 1132 Size: 97 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PfoI T'CCnGG_A Cuts at: 2706 2706 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PmlI CAC'GTG Cuts at: 2671 2671 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2174 2174 Size: 5596 PshAI GACnn'nnGTC Cuts at: 1613 2421 1613 Size: 808 4788 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2299 2299 Size: 5596 SacI G_AGCT'C Cuts at: 2736 2736 Size: 5596 SacII CC_GC'GG Cuts at: 2182 2275 2320 2404 2422 2182 Size: 93 45 84 18 5356 Fragments arranged by size: 5356 93 84 45 18 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SanDI GG'GwC_CC Cuts at: 2174 2174 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SexAI A'CCwGG_T Cuts at: 2649 2649 Size: 5596 SfcI C'TryA_G Cuts at: 826 1045 2944 4079 4760 4951 826 Size: 219 1899 1135 681 191 1471 Fragments arranged by size: 1899 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2103 2103 Size: 5596 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2170 2187 2923 1872 Size: 298 17 736 4545 Fragments arranged by size: 4545 736 298 17 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 2870 3700 4601 4863 820 Size: 197 926 285 642 830 901 262 1553 Fragments arranged by size: 1553 926 901 830 642 285 262 197 SnaBI TAC'GTA Cuts at: 493 2641 3387 493 Size: 2148 746 2702 Fragments arranged by size: 2702 2148 746 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SrfI GCCC'GGGC Cuts at: 2187 2187 Size: 5596 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2466 3035 513 Size: 552 1401 569 3074 Fragments arranged by size: 3074 1401 569 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 2275 3623 3640 3793 3978 4423 Size: 1255 462 233 1348 17 153 185 445 Cuts at: 4423 325 Size: 1498 Fragments arranged by size: 1498 1348 1255 462 445 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2761 2761 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2168 2185 2921 1870 Size: 298 17 736 4545 Fragments arranged by size: 4545 736 298 17 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbvCI BfrBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI SfiI SwaI