(Circular) (Six-base) MAPSORT of: pFN21AE4253.seq Check: 7651 from: 1 to: 7744 pFN21AE4253 7744 bp With 182 enzymes: SgfI * March 28, 2012 13:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2866 2866 Size: 7744 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5554 278 Size: 53 83 186 936 4018 2468 Fragments arranged by size: 4018 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2104 5087 1937 Size: 167 2983 4594 Fragments arranged by size: 4594 2983 167 Acc65I G'GTAC_C Cuts at: 2444 5065 2444 Size: 2621 5123 AclI AA'CG_TT Cuts at: 1563 5871 6244 1563 Size: 4308 373 3063 Fragments arranged by size: 4308 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 3399 3655 4014 4054 4625 5807 7191 Size: 567 1200 256 359 40 571 1182 1384 Cuts at: 7191 7459 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1200 1182 571 567 359 268 256 40 AflII C'TTAA_G Cuts at: 820 1017 2503 820 Size: 197 1486 6061 Fragments arranged by size: 6061 1486 197 AflIII A'CryG_T Cuts at: 1203 2404 6643 7620 1203 Size: 1201 4239 977 1327 Fragments arranged by size: 4239 1327 1201 977 AgeI A'CCGG_T Cuts at: 6575 6575 Size: 7744 AhdI GACnn_n'nnGTC Cuts at: 6473 6473 Size: 7744 AleI CACnn'nnGTG Cuts at: 1166 2918 1166 Size: 1752 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7744 AlwNI CAG_nnn'CTG Cuts at: 4415 5487 7059 4415 Size: 1072 1572 5100 Fragments arranged by size: 5100 1572 1072 ApaLI G'TGCA_C Cuts at: 2264 5801 6957 2264 Size: 3537 1156 3051 Fragments arranged by size: 3537 3051 1156 ApoI r'AATT_y Cuts at: 1493 2525 2547 2608 2699 3113 3923 4471 4536 Size: 1032 22 61 91 414 810 548 65 Cuts at: 4536 5053 5279 5431 7540 1493 Size: 517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1032 810 548 517 414 226 152 91 65 61 22 AseI AT'TA_AT Cuts at: 160 1032 3140 3144 6298 160 Size: 872 2108 4 3154 1606 Fragments arranged by size: 3154 2108 1606 872 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7744 AvaI C'yCGr_G Cuts at: 1870 1943 4489 5069 1870 Size: 73 2546 580 4545 Fragments arranged by size: 4545 2546 580 73 AvrII C'CTAG_G Cuts at: 2095 2095 Size: 7744 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4491 4524 4491 Size: 33 7711 BamHI G'GATC_C Cuts at: 4875 5074 5441 4875 Size: 199 367 7178 Fragments arranged by size: 7178 367 199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2444 5065 6520 618 Size: 325 217 627 657 2621 1455 1842 Fragments arranged by size: 2621 1842 1455 657 627 325 217 BanII G_rGCy'C Cuts at: 1391 2363 2603 3447 5063 1391 Size: 972 240 844 1616 4072 Fragments arranged by size: 4072 1616 972 844 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 3661 5031 928 Size: 971 198 1564 1370 3641 Fragments arranged by size: 3641 1564 1370 971 198 BbvCI CC'TCA_GC Cuts at: 3806 3806 Size: 7744 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4573 4607 5935 5969 1903 Size: 34 2636 34 1328 34 3678 Fragments arranged by size: 3678 2636 1328 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2758 2781 5636 6846 2758 Size: 23 2855 1210 3656 Fragments arranged by size: 3656 2855 1210 23 BclI T'GATC_A Cuts at: 1548 3843 1548 Size: 2295 5449 BfrBI ATG'CAT Cuts at: 3601 3601 Size: 7744 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3335 4833 6355 Size: 107 122 71 631 605 1663 1498 1522 Cuts at: 6355 136 Size: 1525 Fragments arranged by size: 1663 1525 1522 1498 631 605 122 107 71 BglII A'GATC_T Cuts at: 3218 3942 7739 3218 Size: 724 3797 3223 Fragments arranged by size: 3797 3223 724 BlpI GC'TnA_GC Cuts at: 5161 5161 Size: 7744 Bme1580I G_kGCm'C Cuts at: 1790 2268 3567 4468 4932 5805 6961 1790 Size: 478 1299 901 464 873 1156 2573 Fragments arranged by size: 2573 1299 1156 901 873 478 464 BmgBI CAC'GTC Cuts at: 1318 2108 2263 1318 Size: 790 155 6799 Fragments arranged by size: 6799 790 155 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3442 4754 5475 Size: 416 53 83 399 24 2025 1312 721 Cuts at: 5475 6433 442 Size: 958 1753 Fragments arranged by size: 2025 1753 1312 958 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7744 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4948 4948 Size: 7744 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4372 4461 4488 6404 1239 Size: 3133 89 27 1916 2579 Fragments arranged by size: 3133 2579 1916 89 27 Bpu10I CC'TnA_GC Cuts at: 3806 3806 Size: 7744 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3629 3719 5869 6734 7032 3629 Size: 90 2150 865 298 4341 Fragments arranged by size: 4341 2150 865 298 90 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3605 4438 4987 6407 882 Size: 617 2106 833 549 1420 2219 Fragments arranged by size: 2219 2106 1420 833 617 549 BsaAI yAC'GTr Cuts at: 493 1204 4084 5535 493 Size: 711 2880 1451 2702 Fragments arranged by size: 2880 2702 1451 711 BsaBI GATnn'nnATC Cuts at: 7738 7738 Size: 7744 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3531 5551 5933 6568 Size: 53 83 186 936 1998 2020 382 635 Cuts at: 6568 275 Size: 1451 Fragments arranged by size: 2020 1998 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6177 6575 6849 6996 7407 6177 Size: 398 274 147 411 6514 Fragments arranged by size: 6514 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3434 3464 1178 Size: 30 165 30 2031 30 5458 Fragments arranged by size: 5458 2031 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3338 4467 1186 Size: 2152 1129 4463 Fragments arranged by size: 4463 2152 1129 BseYI C'CCAG_C Cuts at: 3713 6947 7443 3713 Size: 3234 496 4014 Fragments arranged by size: 4014 3234 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3507 4061 1163 Size: 573 1771 554 4846 Fragments arranged by size: 4846 1771 573 554 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5194 5955 6983 1540 Size: 275 186 3193 761 1028 2301 Fragments arranged by size: 3193 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 3685 4058 5805 5890 6961 2268 Size: 1417 373 1747 85 1071 3051 Fragments arranged by size: 3051 1747 1417 1071 373 85 BsmI GAATG_Cn' Cuts at: 2230 5255 5348 2230 Size: 3025 93 4626 Fragments arranged by size: 4626 3025 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2363 2603 3447 3567 3685 4058 Size: 399 478 95 240 844 120 118 373 Cuts at: 4058 4468 4932 5063 5805 5890 6961 1391 Size: 410 464 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 844 742 478 464 410 399 373 240 131 120 118 95 85 BspEI T'CCGG_A Cuts at: 7407 7407 Size: 7744 BspHI T'CATG_A Cuts at: 3472 5633 7363 3472 Size: 2161 1730 3853 Fragments arranged by size: 3853 2161 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2866 3714 5099 844 Size: 2022 848 1385 3489 Fragments arranged by size: 3489 2022 1385 848 BsrBI CCG'CTC Cuts at: 1394 1823 2973 5631 1394 Size: 429 1150 2658 3507 Fragments arranged by size: 3507 2658 1150 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2677 6239 6413 65 Size: 1183 1429 3562 174 1396 Fragments arranged by size: 3562 1429 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6388 6575 1700 Size: 110 4578 187 2869 Fragments arranged by size: 4578 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4554 96 Size: 4458 3286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7744 BssSI C'ACGA_G Cuts at: 4577 5804 6816 4577 Size: 1227 1012 5505 Fragments arranged by size: 5505 1227 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7744 BstBI TT'CG_AA Cuts at: 6548 6548 Size: 7744 BstEII G'GTnAC_C Cuts at: 1184 3874 1184 Size: 2690 5054 BstXI CCAn_nnnn'nTGG Cuts at: 2186 2518 4654 2186 Size: 332 2136 5276 Fragments arranged by size: 5276 2136 332 BstYI r'GATC_y Cuts at: 1972 2135 3218 3420 3490 3942 4320 4875 5074 Size: 163 1083 202 70 452 378 555 199 Cuts at: 5074 5441 5826 5843 7295 7381 7393 7739 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1083 555 452 385 378 367 346 202 199 163 86 70 17 12 Bsu36I CC'TnA_GG Cuts at: 4923 4923 Size: 7744 BtgI C'CryG_G Cuts at: 513 1065 2004 2752 3437 3963 4314 513 Size: 552 939 748 685 526 351 3943 Fragments arranged by size: 3943 939 748 685 552 526 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7005 Fragments arranged by size: 7005 683 56 BtsI GCAGTG_nn' Cuts at: 4353 4857 4873 5260 6054 6074 4353 Size: 504 16 387 794 20 6023 Fragments arranged by size: 6023 794 504 387 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7744 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7709 DraI TTT'AAA Cuts at: 2706 5048 2706 Size: 2342 5402 DraIII CAC_nnn'GTG Cuts at: 4084 4084 Size: 7744 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6751 809 Size: 507 5435 1802 Fragments arranged by size: 5435 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 5191 6080 Size: 54 1425 50 275 18 204 3157 889 Cuts at: 6080 7709 8 Size: 1629 43 Fragments arranged by size: 3157 1629 1425 889 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5191 1537 Size: 275 3379 4090 Fragments arranged by size: 4090 3379 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2218 2351 3045 3713 3990 5674 1353 Size: 865 133 694 668 277 1684 3423 Fragments arranged by size: 3423 1684 865 694 668 277 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3353 4313 6331 6705 6851 387 Size: 1064 138 1764 960 2018 374 146 1280 Fragments arranged by size: 2018 1764 1280 1064 960 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 3399 3655 4014 4054 4372 4461 Size: 393 567 1200 256 359 40 318 89 Cuts at: 4461 4488 4625 5807 6404 7191 7459 1239 Size: 27 137 1182 597 787 268 1524 Fragments arranged by size: 1524 1200 1182 787 597 567 393 359 318 268 256 137 89 40 27 EcoO109I rG'GnC_Cy Cuts at: 2537 3694 4728 4835 4951 2537 Size: 1157 1034 107 116 5330 Fragments arranged by size: 5330 1157 1034 116 107 EcoRI G'AATT_C Cuts at: 3113 3923 5053 5431 3113 Size: 810 1130 378 5426 Fragments arranged by size: 5426 1130 810 378 EcoRV GAT'ATC Cuts at: 2908 2908 Size: 7744 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2518 2706 4630 2518 Size: 188 1924 5632 Fragments arranged by size: 5632 1924 188 FspI TGC'GCA Cuts at: 6250 6250 Size: 7744 HaeII r_GCGC'y Cuts at: 1678 4787 6891 1678 Size: 3109 2104 2531 Fragments arranged by size: 3109 2531 2104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2995 3027 3383 3415 3542 Size: 32 135 32 1423 32 356 32 127 Cuts at: 3542 3574 3991 4023 4421 4453 6385 6417 6459 Size: 32 417 32 398 32 1932 32 42 Cuts at: 6459 6491 1373 Size: 32 2626 Fragments arranged by size: 2626 1932 1423 417 398 356 135 127 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5088 5334 669 Size: 988 281 3150 246 3079 Fragments arranged by size: 3150 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3403 748 Size: 2655 5089 HpaI GTT'AAC Cuts at: 5334 5334 Size: 7744 KpnI G_GTAC'C Cuts at: 2448 5069 2448 Size: 2621 5123 MfeI C'AATT_G Cuts at: 4269 5343 4269 Size: 1074 6670 MluI A'CGCG_T Cuts at: 7620 7620 Size: 7744 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3729 4229 4374 6858 7042 1305 Size: 2424 500 145 2484 184 2007 Fragments arranged by size: 2484 2424 2007 500 184 145 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 10 Size: 54 1425 343 204 5718 Fragments arranged by size: 5718 1425 343 204 54 MslI CAynn'nnrTG Cuts at: 518 1166 2918 3774 3968 4016 4652 5702 6061 Size: 648 1752 856 194 48 636 1050 359 Cuts at: 6061 6220 518 Size: 159 2042 Fragments arranged by size: 2042 1752 1050 856 648 636 359 194 159 48 MspA1I CmG'CkG Cuts at: 1606 3992 4372 4378 4421 5160 5837 6985 7230 Size: 2386 380 6 43 739 677 1148 245 Cuts at: 7230 1606 Size: 2120 Fragments arranged by size: 2386 2120 1148 739 677 380 245 43 6 NcoI C'CATG_G Cuts at: 513 1065 2004 3963 4314 513 Size: 552 939 1959 351 3943 Fragments arranged by size: 3943 1959 939 552 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 7744 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7744 NotI GC'GGCC_GC Cuts at: 5191 5191 Size: 7744 NsiI A_TGCA'T Cuts at: 3603 3603 Size: 7744 NspI r_CATG'y Cuts at: 1132 1229 3262 3605 5102 6647 1132 Size: 97 2033 343 1497 1545 2229 Fragments arranged by size: 2229 2033 1545 1497 343 97 PacI TTA_AT'TAA Cuts at: 3144 3144 Size: 7744 PasI CC'CwG_GG Cuts at: 1348 2541 3495 4522 1348 Size: 1193 954 1027 4570 Fragments arranged by size: 4570 1193 1027 954 PciI A'CATG_T Cuts at: 6643 6643 Size: 7744 PflMI CCAn_nnn'nTGG Cuts at: 1108 3068 1108 Size: 1960 5784 PmeI GTTT'AAAC Cuts at: 5048 5048 Size: 7744 PmlI CAC'GTG Cuts at: 4084 4084 Size: 7744 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5813 5845 7326 7358 5813 Size: 32 1481 32 6199 Fragments arranged by size: 6199 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4728 4951 4728 Size: 223 7521 PshAI GACnn'nnGTC Cuts at: 1613 4771 1613 Size: 3158 4586 PsiI TTA'TAA Cuts at: 5314 5314 Size: 7744 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2267 2299 2267 Size: 32 7712 PstI C_TGCA'G Cuts at: 830 3461 3737 4360 4377 4420 5096 830 Size: 2631 276 623 17 43 676 3478 Fragments arranged by size: 3478 2631 676 623 276 43 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7744 PvuII CAG'CTG Cuts at: 3992 4372 4378 4421 3992 Size: 380 6 43 7315 Fragments arranged by size: 7315 380 43 6 SalI G'TCGA_C Cuts at: 1936 5086 1936 Size: 3150 4594 SapI GCTCTTCn'nnn_ Cuts at: 2218 2351 3713 2218 Size: 133 1362 6249 Fragments arranged by size: 6249 1362 133 SbfI CC_TGCA'GG Cuts at: 5096 5096 Size: 7744 ScaI AGT'ACT Cuts at: 5992 7704 5992 Size: 1712 6032 SexAI A'CCwGG_T Cuts at: 4344 4344 Size: 7744 SfcI C'TryA_G Cuts at: 826 1045 2101 2467 3457 3733 4165 4356 4373 Size: 219 1056 366 990 276 432 191 17 Cuts at: 4373 4416 5092 6227 6908 7099 826 Size: 43 676 1135 681 191 1471 Fragments arranged by size: 1471 1135 1056 990 681 676 432 366 276 219 191 191 43 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7744 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7744 SmaI CCC'GGG Cuts at: 1872 4491 5071 1872 Size: 2619 580 4545 Fragments arranged by size: 4545 2619 580 SmlI C'TyrA_G Cuts at: 820 1017 1943 2503 3608 3698 5848 6749 7011 Size: 197 926 560 1105 90 2150 901 262 Cuts at: 7011 820 Size: 1553 Fragments arranged by size: 2150 1553 1105 926 901 560 262 197 90 SnaBI TAC'GTA Cuts at: 493 5535 493 Size: 5042 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7744 SphI G_CATG'C Cuts at: 1132 5102 1132 Size: 3970 3774 SspI AAT'ATT Cuts at: 5 52 2309 3042 5668 5 Size: 47 2257 733 2626 2081 Fragments arranged by size: 2626 2257 2081 733 47 StuI AGG'CCT Cuts at: 1517 3506 1517 Size: 1989 5755 StyI C'CwwG_G Cuts at: 513 1065 2004 2095 2812 3963 4314 4452 4623 Size: 552 939 91 717 1151 351 138 171 Cuts at: 4623 4828 4890 5183 513 Size: 205 62 293 3074 Fragments arranged by size: 3074 1151 939 717 552 351 293 205 171 138 91 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2705 3316 4654 5771 5788 5941 6126 Size: 1255 1125 611 1338 1117 17 153 185 Cuts at: 6126 6571 325 Size: 445 1498 Fragments arranged by size: 1498 1338 1255 1125 1117 611 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3930 4554 5990 Size: 274 80 33 51 1443 1953 624 1436 Cuts at: 5990 7702 96 Size: 1712 138 Fragments arranged by size: 1953 1712 1443 1436 624 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3434 3466 4673 4705 7704 7736 3434 Size: 32 1207 32 2999 32 3442 Fragments arranged by size: 3442 2999 1207 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7744 XbaI T'CTAG_A Cuts at: 5080 5080 Size: 7744 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4312 4312 Size: 7744 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7744 XmaI C'CCGG_G Cuts at: 1870 4489 5069 1870 Size: 2619 580 4545 Fragments arranged by size: 4545 2619 580 XmnI GAAnn'nnTTC Cuts at: 2550 5873 2550 Size: 3323 4421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5552 276 Size: 53 83 186 936 4018 2468 Fragments arranged by size: 4018 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI