################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BbeI BcgI BciVI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI BspEI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AcsI 536 AAATTC [GA]^AATT[CT] AflIII 529 ACGTGT A^C[GA][CT]GT AluI 558 AGCT AG^CT Alw44I 422 GTGCAC G^TGCAC ApaLI 422 GTGCAC G^TGCAC ApoI 536 AAATTC [GA]^AATT[CT] Asp700I 70 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 371 TGGCCA TGG^CCA BanI 721 GGTGCC G^G[CT][GA]CC BanII 321 GGGCTC G[GA]GC[CT]^C BbrPI 528 CACGTG CAC^GTG BbsI 85 GTCTTC GAAGAC(2/6) BbvCI 683 CCTCAGC CCTCAGC(-5/-2) BbvI 757 GCTGC GCAGC(8/12) BclI 140 TGATCA T^GATCA BfaI 151 CTAG C^TAG BglI 346 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 85 GTCTTC GAAGAC(2/6) BsaAI 528 CACGTG [CT]AC^GT[GA] BsaBI 141 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 438 GGTCTC GGTCTC(1/5) BseRI 610 CTCCTC GAGGAG(10/8) BsmAI 439 GTCTC GTCTC(1/5) BsmFI 309 GTCCC GGGAC(10/14) BsmI 605 GCATTC GAATGC(1/-1) Bsp1407I 294 TGTACA T^GTACA BspDI 517 ATCGAT AT^CGAT BsrBI 464 CCGCTC CCGCTC(-3/-3) BsrDI 923 CATTGC GCAATG(2/0) BsrGI 294 TGTACA T^GTACA BstXI 587 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 559 GCTTGC GC[ACGT]^[ACGT]GC ClaI 517 ATCGAT AT^CGAT DraIII 327 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 317 CCATGG C^C[GA][CT]GG EarI 79 CTCTTC CTCTTC(1/4) FauI 384 CCCGC CCCGC(4/6) FbaI 140 TGATCA T^GATCA Fnu4HI 757 GCTGC GC^[ACGT]GC HgaI 416 GCGTC GACGC(5/10) ItaI 757 GCTGC GC^[ACGT]GC Ksp632I 79 CTCTTC CTCTTC(1/4) MaeI 151 CTAG C^TAG MaeII 529 ACGT A^CGT MamI 141 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MspA1I 385 CCGCTG C[AC]G^C[GT]G NcoI 317 CCATGG C^CATGG NdeI 356 CATATG CA^TATG PmaCI 528 CACGTG CAC^GTG PmlI 528 CACGTG CAC^GTG PstI 490 CTGCAG CTGCA^G SfcI 490 CTGCAG C^T[GA][CT]AG SspBI 294 TGTACA T^GTACA TaqI 518 TCGA T^CGA TseI 757 GCTGC G^C[AT]GC Tsp45I 448 GTGAC ^GT[GC]AC TspRI 572 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 518 TCGA T^CGA XcmI 587 CCATCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 70 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AspHI 337 GTGCTC G[AT]GC[AT]^C 422 GTGCAC BsiHKAI 337 GTGCTC G[AT]GC[AT]^C 422 GTGCAC BspHI 404 TCATGA T^CATGA 578 TCATGA BsrI 54 CCAGT ACTGG(1/-1) 479 CCAGT DpnII 141 GATC ^GATC 520 GATC EaeI 344 TGGCCG [CT]^GGCC[GA] 371 TGGCCA EcoT14I 186 CCTTGG C^C[AT][AT]GG 317 CCATGG HaeIII 345 GGCC GG^CC 372 GGCC MboI 141 GATC ^GATC 520 GATC MseI 164 TTAA T^TAA 871 TTAA MwoI 346 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 424 GCACGGATGGC NdeII 141 GATC ^GATC 520 GATC NlaIV 322 GGCTCC GG[ACGT]^[ACGT]CC 721 GGTGCC RcaI 404 TCATGA T^CATGA 578 TCATGA Sau3AI 141 GATC ^GATC 520 GATC StyI 186 CCTTGG C^C[AT][AT]GG 317 CCATGG Tru9I 164 TTAA T^TAA 871 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AfaI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI DdeI EcoRII FokI HinfI HphI MaeIII MboII MnlI MslI MvaI NlaIII RsaI ScrFI SfaNI TfiI Tsp509I