################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BciVI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PvuII RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 66 GTCGAC GT^[AC][GT]AC AcsI 892 GAATTC [GA]^AATT[CT] AflIII 140 ACGTGT A^C[GA][CT]GT AlwNI 1032 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 892 GAATTC [GA]^AATT[CT] AseI 462 ATTAAT AT^TAAT AsnI 462 ATTAAT AT^TAAT BanI 922 GGTGCC G^G[CT][GA]CC BbsI 1310 GAAGAC GAAGAC(2/6) BcgI 1014 CGAGACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 372 TGATCA T^GATCA BfaI 898 CTAG C^TAG BglII 1429 AGATCT A^GATCT BmyI 963 GGGCAC G[AGT]GC[ACT]^C BpuAI 1310 GAAGAC GAAGAC(2/6) BsaI 1015 GAGACC GGTCTC(1/5) BsgI 1403 GTGCAG GTGCAG(16/14) Bsp1286I 963 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 210 TGTACA T^GTACA BspHI 986 TCATGA T^CATGA BsrBI 16 GAGCGG CCGCTC(-3/-3) BsrGI 210 TGTACA T^GTACA BstAPI 388 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 715 GGTTACC G^GT[ACGT]ACC BstPI 715 GGTTACC G^GT[ACGT]ACC DraI 645 TTTAAA TTT^AAA DraII 125 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1277 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 125 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 715 GGTTACC G^GT[ACGT]ACC EcoRI 892 GAATTC G^AATTC EcoRV 901 GATATC GAT^ATC EcoT14I 951 CCTTGG C^C[AT][AT]GG FauI 114 CCCGC CCCGC(4/6) FbaI 372 TGATCA T^GATCA HaeII 56 GGCGCT [GA]GCGC^[CT] HincII 66 GTCGAC GT[CT]^[GA]AC HindII 66 GTCGAC GT[CT]^[GA]AC MaeI 898 CTAG C^TAG MaeII 140 ACGT A^CGT MslI 330 CATTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PpuMI 125 GGGACCC [GA]G^G[AT]CC[CT] PshBI 462 ATTAAT AT^TAAT PstI 1038 CTGCAG CTGCA^G PvuI 159 CGATCG CGAT^CG RcaI 986 TCATGA T^CATGA SalI 66 GTCGAC G^TCGAC SspBI 210 TGTACA T^GTACA StyI 951 CCTTGG C^C[AT][AT]GG SwaI 644 ATTTAAAT ATTT^AAAT Tsp45I 1273 GTGAC ^GT[GC]AC XcmI 1094 CCAACCTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 90 GATCC GGATC(4/5) 1034 GGATC BbvCI 1063 CCTCAGC CCTCAGC(-5/-2) 916 GCTGAGG BcnI 76 CCGGG CC^[GC]GG 123 CCGGG BsaJI 131 CCCAGG C^C[ACGT][ACGT]GG 951 CCTTGG BsiEI 22 CGGTCG CG[GA][CT]^CG 159 CGATCG BsmFI 125 GGGAC GGGAC(10/14) 1112 GGGAC BsrI 364 ACTGG ACTGG(1/-1) 1358 CCAGT HapII 76 CCGG C^CGG 123 CCGG HinfI 759 GAATC G^A[ACGT]TC 1218 GAATC HpaII 76 CCGG C^CGG 123 CCGG MspI 76 CCGG C^CGG 123 CCGG NciI 76 CCGGG CC^[GC]GG 123 CCGGG PflMI 1019 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1094 CCAACCTCTGG SfcI 183 CTACAG C^T[GA][CT]AG 1038 CTGCAG SspI 459 AATATT AAT^ATT 749 AATATT TfiI 759 GAATC G^A[AT]TC 1218 GAATC Van91I 1019 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1094 CCAACCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BbvI BsiYI BslI BsmAI BsrDI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII