################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## Acc65I AccII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI CelII Cfr10I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HaeII HindIII HpaI KasI KpnI KspI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 2134 GACGTC GACGT^C AccIII 2095 TCCGGA T^CCGGA AcyI 2134 GACGTC G[GA]^CG[CT]C AocI 1590 CCTTAGG CC^T[ACGT]AGG AseI 1429 ATTAAT AT^TAAT AsnI 1429 ATTAAT AT^TAAT AvaI 1532 CTCGGG C^[CT]CG[GA]G BcgI 1721 GCACAGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1037 TGATCA T^GATCA BcnI 1095 CCGGG CC^[GC]GG BsaBI 468 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2134 GACGTC G[GA]^CG[CT]C BsaWI 2095 TCCGGA [AT]^CCGG[AT] BseAI 2095 TCCGGA T^CCGGA BsoBI 1532 CTCGGG C^[CT]CG[GA]G BspDI 1222 ATCGAT AT^CGAT BspEI 2095 TCCGGA T^CCGGA BspMI 1913 ACCTGC ACCTGC(4/8) BsrDI 1994 GCAATG GCAATG(2/0) Bst1107I 1392 GTATAC GTA^TAC BstAPI 1125 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 2252 GGATCT [GA]^GATC[CT] BstZ17I 1392 GTATAC GTA^TAC Bsu36I 1590 CCTTAGG CC^T[ACGT]AGG CfoI 1963 GCGC GCG^C ClaI 1222 ATCGAT AT^CGAT DraIII 751 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1590 CCTTAGG CC^T[ACGT]AGG FbaI 1037 TGATCA T^GATCA HgaI 856 GACGC GACGC(5/10) HhaI 1963 GCGC GCG^C Hin1I 2134 GACGTC G[GA]^CG[CT]C HinP1I 1963 GCGC G^CGC HincII 2197 GTTGAC GT[CT]^[GA]AC HindII 2197 GTTGAC GT[CT]^[GA]AC MamI 468 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 314 CAATTG C^AATTG MflI 2252 GGATCT [GA]^GATC[CT] MroI 2095 TCCGGA T^CCGGA MunI 314 CAATTG C^AATTG NciI 1095 CCGGG CC^[GC]GG PshAI 831 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1429 ATTAAT AT^TAAT SmlI 1283 CTCAAG C^T[CT][GA]AG XhoII 2252 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1392 GTATAC GT^[AC][GT]AC 1633 GTAGAC AfaI 226 GTAC GT^AC 1155 GTAC AflIII 933 ACACGT A^C[GA][CT]GT 1051 ACACGT BsmAI 425 GAGAC GTCTC(1/5) 1809 GTCTC BsmI 774 GCATTC GAATGC(1/-1) 1551 GCATTC BspHI 48 TCATGA T^CATGA 410 TCATGA EarI 1064 GAAGAG CTCTTC(1/4) 1420 GAAGAG EcoNI 1531 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1877 CCTTGCAGAGG EcoRV 406 GATATC GAT^ATC 597 GATATC HapII 1095 CCGG C^CGG 2096 CCGG HpaII 1095 CCGG C^CGG 2096 CCGG Ksp632I 1064 GAAGAG CTCTTC(1/4) 1420 GAAGAG MspA1I 996 CAGCTG C[AC]G^C[GT]G 2024 CAGCTG MspI 1095 CCGG C^CGG 2096 CCGG NcoI 341 CCATGG C^CATGG 1842 CCATGG NspI 534 ACATGC [GA]CATG^[CT] 698 GCATGT PvuII 996 CAGCTG CAG^CTG 2024 CAGCTG RcaI 48 TCATGA T^CATGA 410 TCATGA RsaI 226 GTAC GT^AC 1155 GTAC SspI 850 AATATT AAT^ATT 1119 AATATT StuI 357 AGGCCT AGG^CCT 2150 AGGCCT Tsp45I 757 GTGAC ^GT[GC]AC 2111 GTCAC XcmI 539 CCATAACAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2222 CCAGCATGGTTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AvaII BalI BbvI BfaI BsaJI BsgI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I