(Circular) (Six-base) MAPSORT of: pFN21AE3291.seq Check: 8507 from: 1 to: 6964 pFN21AE3291 6964 bp With 182 enzymes: SgfI * March 28, 2012 12:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4143 4774 278 Size: 53 83 186 936 2607 631 2468 Fragments arranged by size: 2607 2468 936 631 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 3639 4307 1937 Size: 1461 241 668 4594 Fragments arranged by size: 4594 1461 668 241 Acc65I G'GTAC_C Cuts at: 4285 4285 Size: 6964 AclI AA'CG_TT Cuts at: 1563 5091 5464 1563 Size: 3528 373 3063 Fragments arranged by size: 3528 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2423 3128 5027 6411 6679 1632 Size: 791 705 1899 1384 268 1917 Fragments arranged by size: 1917 1899 1384 791 705 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6767 AflIII A'CryG_T Cuts at: 1203 2938 3056 5863 6840 1203 Size: 1735 118 2807 977 1327 Fragments arranged by size: 2807 1735 1327 977 118 AgeI A'CCGG_T Cuts at: 5795 5795 Size: 6964 AhdI GACnn_n'nnGTC Cuts at: 5693 5693 Size: 6964 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6964 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3180 3212 3180 Size: 32 6932 AlwNI CAG_nnn'CTG Cuts at: 2599 3003 3476 4707 6279 2599 Size: 404 473 1231 1572 3284 Fragments arranged by size: 3284 1572 1231 473 404 ApaLI G'TGCA_C Cuts at: 5021 6177 5021 Size: 1156 5808 ApoI r'AATT_y Cuts at: 1493 3254 3495 3677 4273 4499 4651 6760 1493 Size: 1761 241 182 596 226 152 2109 1697 Fragments arranged by size: 2109 1761 1697 596 241 226 182 152 AseI AT'TA_AT Cuts at: 160 1032 3435 5518 160 Size: 872 2403 2083 1606 Fragments arranged by size: 2403 2083 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 AvaI C'yCGr_G Cuts at: 1870 1943 3537 4289 1870 Size: 73 1594 752 4545 Fragments arranged by size: 4545 1594 752 73 BamHI G'GATC_C Cuts at: 4294 4661 4294 Size: 367 6597 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4285 5740 618 Size: 325 217 627 2498 1455 1842 Fragments arranged by size: 2498 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4283 1391 Size: 2892 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5993 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 5155 5189 1903 Size: 34 1778 34 1406 34 3678 Fragments arranged by size: 3678 1778 1406 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4856 6066 4856 Size: 1210 5754 BclI T'GATC_A Cuts at: 1548 3042 1548 Size: 1494 5470 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5575 136 Size: 107 122 71 631 605 3903 1525 Fragments arranged by size: 3903 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6959 6959 Size: 6964 BlpI GC'TnA_GC Cuts at: 4381 4381 Size: 6964 Bme1580I G_kGCm'C Cuts at: 1790 5025 6181 1790 Size: 3235 1156 2573 Fragments arranged by size: 3235 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2941 3059 4177 1318 Size: 1623 118 1118 4105 Fragments arranged by size: 4105 1623 1118 118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3806 4695 5653 Size: 416 53 83 399 24 2389 889 958 Cuts at: 5653 442 Size: 1753 Fragments arranged by size: 2389 1753 958 889 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6964 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3301 3601 3301 Size: 300 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5624 1239 Size: 4385 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3273 5089 5954 6252 3273 Size: 1816 865 298 3985 Fragments arranged by size: 3985 1816 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5627 882 Size: 617 4128 2219 Fragments arranged by size: 4128 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4755 493 Size: 711 3551 2702 Fragments arranged by size: 3551 2702 711 BsaBI GATnn'nnATC Cuts at: 2477 6958 2477 Size: 4481 2483 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4140 4771 5153 5788 Size: 53 83 186 936 2607 631 382 635 Cuts at: 5788 275 Size: 1451 Fragments arranged by size: 2607 1451 936 635 631 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4100 5397 5795 6069 6216 6627 4100 Size: 1297 398 274 147 411 4437 Fragments arranged by size: 4437 1297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3761 3791 1178 Size: 30 165 30 2358 30 4351 Fragments arranged by size: 4351 2358 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6964 BseYI C'CCAG_C Cuts at: 4216 6167 6663 4216 Size: 1951 496 4517 Fragments arranged by size: 4517 1951 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2175 2560 2607 2949 3122 4190 1163 Size: 573 439 385 47 342 173 1068 3937 Fragments arranged by size: 3937 1068 573 439 385 342 173 47 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4414 5175 6203 1540 Size: 275 186 2413 761 1028 2301 Fragments arranged by size: 2413 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5025 5110 6181 5025 Size: 85 1071 5808 Fragments arranged by size: 5808 1071 85 BsmI GAATG_Cn' Cuts at: 2779 3556 4475 4568 2779 Size: 777 919 93 5175 Fragments arranged by size: 5175 919 777 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4283 5025 5110 6181 1391 Size: 399 2493 742 85 1071 2174 Fragments arranged by size: 2493 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 4100 6627 4100 Size: 2527 4437 BspHI T'CATG_A Cuts at: 2053 2415 4853 6583 2053 Size: 362 2438 1730 2434 Fragments arranged by size: 2438 2434 1730 362 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3927 4319 844 Size: 3083 392 3489 Fragments arranged by size: 3489 3083 392 BsrBI CCG'CTC Cuts at: 1394 1823 4851 1394 Size: 429 3028 3507 Fragments arranged by size: 3507 3028 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4006 5459 5633 65 Size: 1183 2758 1453 174 1396 Fragments arranged by size: 2758 1453 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5608 5795 1700 Size: 110 3798 187 2869 Fragments arranged by size: 3798 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6964 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6964 BssSI C'ACGA_G Cuts at: 5024 6036 5024 Size: 1012 5952 BstAPI GCAn_nnn'nTGC Cuts at: 794 3136 794 Size: 2342 4622 BstBI TT'CG_AA Cuts at: 5768 5768 Size: 6964 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6964 BstYI r'GATC_y Cuts at: 1972 4257 4294 4661 5046 5063 6515 6601 6613 Size: 2285 37 367 385 17 1452 86 12 Cuts at: 6613 6959 1972 Size: 346 1977 Fragments arranged by size: 2285 1977 1452 385 367 346 86 37 17 12 BstZ17I GTA'TAC Cuts at: 3399 3399 Size: 6964 Bsu36I CC'TnA_GG Cuts at: 3596 3596 Size: 6964 BtgI C'CryG_G Cuts at: 513 1065 2004 2346 3769 3847 513 Size: 552 939 342 1423 78 3630 Fragments arranged by size: 3630 1423 939 552 342 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6225 Fragments arranged by size: 6225 683 56 BtsI GCAGTG_nn' Cuts at: 2590 3134 3242 4480 5274 5294 2590 Size: 544 108 1238 794 20 4260 Fragments arranged by size: 4260 1238 794 544 108 20 ClaI AT'CG_AT Cuts at: 1554 3228 1554 Size: 1674 5290 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6929 DraI TTT'AAA Cuts at: 4268 4268 Size: 6964 DraIII CAC_nnn'GTG Cuts at: 2761 2761 Size: 6964 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5971 809 Size: 507 4655 1802 Fragments arranged by size: 4655 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2454 3327 3523 Size: 54 1425 50 275 18 624 873 196 Cuts at: 3523 3682 4110 4411 5300 6929 8 Size: 159 428 301 889 1629 43 Fragments arranged by size: 1629 1425 889 873 624 428 301 275 196 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4411 1537 Size: 275 2599 4090 Fragments arranged by size: 4090 2599 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3064 3420 4894 1353 Size: 1711 356 1474 3423 Fragments arranged by size: 3423 1711 1474 356 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3683 5551 5925 6071 387 Size: 1064 138 2094 1868 374 146 1280 Fragments arranged by size: 2094 1868 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 3128 5027 5624 6411 6679 1239 Size: 393 791 705 1899 597 787 268 1524 Fragments arranged by size: 1899 1524 791 787 705 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3540 3886 3540 Size: 346 6618 EcoO109I rG'GnC_Cy Cuts at: 2048 3600 3916 2048 Size: 1552 316 5096 Fragments arranged by size: 5096 1552 316 EcoRI G'AATT_C Cuts at: 4273 4651 4273 Size: 378 6586 EcoRV GAT'ATC Cuts at: 2413 2604 2413 Size: 191 6773 FspI TGC'GCA Cuts at: 5470 5470 Size: 6964 HaeII r_GCGC'y Cuts at: 1678 6111 1678 Size: 4433 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2711 2743 5605 5637 5679 Size: 32 135 32 1139 32 2862 32 42 Cuts at: 5679 5711 1373 Size: 32 2626 Fragments arranged by size: 2862 2626 1139 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4204 4308 4554 669 Size: 988 281 2266 104 246 3079 Fragments arranged by size: 3079 2266 988 281 246 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 6964 HpaI GTT'AAC Cuts at: 4554 4554 Size: 6964 KpnI G_GTAC'C Cuts at: 4289 4289 Size: 6964 MfeI C'AATT_G Cuts at: 2319 4563 2319 Size: 2244 4720 MluI A'CGCG_T Cuts at: 6840 6840 Size: 6964 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3001 6078 6262 1305 Size: 1696 3077 184 2007 Fragments arranged by size: 3077 2007 1696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2456 3684 4112 10 Size: 54 1425 343 624 1228 428 2862 Fragments arranged by size: 2862 1425 1228 624 428 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 2565 2702 2789 3756 4922 5281 Size: 648 1155 244 137 87 967 1166 359 Cuts at: 5281 5440 518 Size: 159 2042 Fragments arranged by size: 2042 1166 1155 967 648 359 244 159 137 87 MspA1I CmG'CkG Cuts at: 1606 3003 4031 4380 5057 6205 6450 1606 Size: 1397 1028 349 677 1148 245 2120 Fragments arranged by size: 2120 1397 1148 1028 677 349 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2346 3847 513 Size: 552 939 342 1501 3630 Fragments arranged by size: 3630 1501 939 552 342 NdeI CA'TA_TG Cuts at: 387 387 Size: 6964 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6964 NotI GC'GGCC_GC Cuts at: 4411 4411 Size: 6964 NspI r_CATG'y Cuts at: 1132 1229 2543 2707 4322 5867 1132 Size: 97 1314 164 1615 1545 2229 Fragments arranged by size: 2229 1615 1545 1314 164 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6964 PciI A'CATG_T Cuts at: 5863 5863 Size: 6964 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6964 PfoI T'CCnGG_A Cuts at: 3099 3099 Size: 6964 PmeI GTTT'AAAC Cuts at: 4268 4268 Size: 6964 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5033 5065 6546 6578 5033 Size: 32 1481 32 5419 Fragments arranged by size: 5419 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2048 3600 3916 2048 Size: 1552 316 5096 Fragments arranged by size: 5096 1552 316 PshAI GACnn'nnGTC Cuts at: 1613 2840 1613 Size: 1227 5737 PsiI TTA'TAA Cuts at: 4534 4534 Size: 6964 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2669 2701 3389 3421 2669 Size: 32 688 32 6212 Fragments arranged by size: 6212 688 32 32 PstI C_TGCA'G Cuts at: 830 2028 3469 3494 3793 4316 830 Size: 1198 1441 25 299 523 3478 Fragments arranged by size: 3478 1441 1198 523 299 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6964 PvuII CAG'CTG Cuts at: 3003 4031 3003 Size: 1028 5936 SalI G'TCGA_C Cuts at: 1936 4306 1936 Size: 2370 4594 SbfI CC_TGCA'GG Cuts at: 4316 4316 Size: 6964 ScaI AGT'ACT Cuts at: 5212 6924 5212 Size: 1712 5252 SfcI C'TryA_G Cuts at: 826 1045 2024 2750 3446 3465 3490 3789 4312 Size: 219 979 726 696 19 25 299 523 Cuts at: 4312 5447 6128 6319 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 726 696 681 523 299 219 191 25 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6964 SmaI CCC'GGG Cuts at: 1872 4291 1872 Size: 2419 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3288 5068 5969 6231 820 Size: 197 926 1345 1780 901 262 1553 Fragments arranged by size: 1780 1553 1345 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4755 493 Size: 4262 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6964 SphI G_CATG'C Cuts at: 1132 4322 1132 Size: 3190 3774 SspI AAT'ATT Cuts at: 5 52 2857 3126 4888 5 Size: 47 2805 269 1762 2081 Fragments arranged by size: 2805 2081 1762 269 47 StuI AGG'CCT Cuts at: 1517 2364 4157 1517 Size: 847 1793 4324 Fragments arranged by size: 4324 1793 847 StyI C'CwwG_G Cuts at: 513 1065 2004 2346 3200 3324 3685 3847 4403 Size: 552 939 342 854 124 361 162 556 Cuts at: 4403 513 Size: 3074 Fragments arranged by size: 3074 939 854 556 552 361 342 162 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3128 3840 3953 4208 4991 5008 5161 Size: 1255 1548 712 113 255 783 17 153 Cuts at: 5161 5346 5791 325 Size: 185 445 1498 Fragments arranged by size: 1548 1498 1255 783 712 445 255 185 153 113 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5210 6922 96 Size: 274 80 33 51 1443 3233 1712 138 Fragments arranged by size: 3233 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2588 2620 3024 3056 3761 3793 6924 6956 2588 Size: 32 404 32 705 32 3131 32 2596 Fragments arranged by size: 3131 2596 705 404 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6964 XbaI T'CTAG_A Cuts at: 4300 4300 Size: 6964 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2551 4234 2551 Size: 1683 5281 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6964 XmaI C'CCGG_G Cuts at: 1870 4289 1870 Size: 2419 4545 XmnI GAAnn'nnTTC Cuts at: 5093 5093 Size: 6964 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4141 4772 276 Size: 53 83 186 936 2607 631 2468 Fragments arranged by size: 2607 2468 936 631 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI