################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BanI BanII BbeI BbrPI BcgI BciVI BfrI BglI BglII BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 162 CCGC CCGC(-3/-1) AlwNI 441 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 88 GTGCTC G[AT]GC[AT]^C AspI 514 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 374 TGGCCA TGG^CCA BamHI 683 GGATCC G^GATCC BcnI 630 CCCGG CC^[GC]GG BmyI 88 GTGCTC G[AGT]GC[ACT]^C BsiHKAI 88 GTGCTC G[AT]GC[AT]^C Bsp1286I 88 GTGCTC G[AGT]GC[ACT]^C BspHI 407 TCATGA T^CATGA BspMI 721 ACCTGC ACCTGC(4/8) BsrDI 125 GCAATG GCAATG(2/0) BsrI 240 ACTGG ACTGG(1/-1) BssSI 109 CTCGTG CACGAG(-5/-1) EaeI 374 TGGCCA [CT]^GGCC[GA] FauI 161 CCCGC CCCGC(4/6) HapII 631 CCGG C^CGG HpaII 631 CCGG C^CGG MluNI 374 TGGCCA TGG^CCA MscI 374 TGGCCA TGG^CCA MspI 631 CCGG C^CGG MwoI 90 GCTCATCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 630 CCCGG CC^[GC]GG NspI 425 ACATGC [GA]CATG^[CT] PflFI 514 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 440 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 407 TCATGA T^CATGA SfaNI 870 GATGC GCATC(5/9) SfcI 888 CTATAG C^T[GA][CT]AG TfiI 543 GATTC G^A[AT]TC Tsp509I 492 AATT ^AATT TspRI 239 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 514 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 440 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvrII 189 CCTAGG C^CTAGG 621 CCTAGG BbvCI 689 CCTCAGC CCTCAGC(-5/-2) 694 GCTGAGG BclI 404 TGATCA T^GATCA 590 TGATCA BfaI 190 CTAG C^TAG 622 CTAG BlnI 189 CCTAGG C^CTAGG 621 CCTAGG BsaI 16 GAGACC GGTCTC(1/5) 936 GAGACC BseRI 228 CTCCTC GAGGAG(10/8) 924 GAGGAG BsgI 166 CTGCAC GTGCAG(16/14) 298 GTGCAG BstEII 144 GGTGACC G^GT[ACGT]ACC 861 GGTGACC BstPI 144 GGTGACC G^GT[ACGT]ACC 861 GGTGACC BstYI 683 GGATCC [GA]^GATC[CT] 822 GGATCT DraII 131 GGGTCCT [GA]G^G[ACGT]CC[CT] 760 GGGACCT EcoO109I 131 GGGTCCT [GA]G^G[ACGT]CC[CT] 760 GGGACCT EcoO65I 144 GGTGACC G^GT[ACGT]ACC 861 GGTGACC FbaI 404 TGATCA T^GATCA 590 TGATCA HaeIII 375 GGCC GG^CC 564 GGCC MaeI 190 CTAG C^TAG 622 CTAG MflI 683 GGATCC [GA]^GATC[CT] 822 GGATCT MspA1I 496 CAGCTG C[AC]G^C[GT]G 692 CAGCTG PpuMI 131 GGGTCCT [GA]G^G[AT]CC[CT] 760 GGGACCT PvuII 496 CAGCTG CAG^CTG 692 CAGCTG XhoII 683 GGATCC [GA]^GATC[CT] 822 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AfaI AluI AlwI AocI AvaII BbsI BbvI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DsaI Eco81I EcoRII EcoT14I Fnu4HI FokI HinfI HphI ItaI MaeIII MboI MboII MnlI MslI MvaI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI StyI TseI Tsp45I