(Circular) (Six-base) MAPSORT of: pFN21AE5430.seq Check: 7531 from: 1 to: 5662 pFN21AE5430 5662 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2735 2735 Size: 5662 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3005 1937 Size: 1068 4594 Acc65I G'GTAC_C Cuts at: 2983 2983 Size: 5662 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3725 5109 5377 1632 Size: 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5465 AflIII A'CryG_T Cuts at: 1203 4561 5538 1203 Size: 3358 977 1327 Fragments arranged by size: 3358 1327 977 AgeI A'CCGG_T Cuts at: 4493 4493 Size: 5662 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 2747 1166 Size: 1581 4081 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5662 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2654 2622 Size: 32 5630 AlwNI CAG_nnn'CTG Cuts at: 2451 3405 4977 2451 Size: 954 1572 3136 Fragments arranged by size: 3136 1572 954 ApaLI G'TGCA_C Cuts at: 3719 4875 3719 Size: 1156 4506 ApoI r'AATT_y Cuts at: 1493 2971 3197 3349 5458 1493 Size: 1478 226 152 2109 1697 Fragments arranged by size: 2109 1697 1478 226 152 AseI AT'TA_AT Cuts at: 160 1032 4216 160 Size: 872 3184 1606 Fragments arranged by size: 3184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2987 1870 Size: 73 1044 4545 Fragments arranged by size: 4545 1044 73 AvrII C'CTAG_G Cuts at: 2194 2626 2194 Size: 432 5230 BamHI G'GATC_C Cuts at: 2688 2992 3359 2688 Size: 304 367 4991 Fragments arranged by size: 4991 367 304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2983 4438 618 Size: 325 217 627 1196 1455 1842 Fragments arranged by size: 1842 1455 1196 627 325 217 BanII G_rGCy'C Cuts at: 1391 2981 1391 Size: 1590 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2656 2710 2749 928 Size: 971 757 54 39 3841 Fragments arranged by size: 3841 971 757 54 39 BbvCI CC'TCA_GC Cuts at: 2695 2700 2695 Size: 5 5657 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3853 3887 1903 Size: 34 1916 34 3678 Fragments arranged by size: 3678 1916 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4764 3554 Size: 1210 4452 BclI T'GATC_A Cuts at: 1548 2409 2595 1548 Size: 861 186 4615 Fragments arranged by size: 4615 861 186 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4273 136 Size: 107 122 71 631 605 2601 1525 Fragments arranged by size: 2601 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5657 5657 Size: 5662 BlpI GC'TnA_GC Cuts at: 3079 3079 Size: 5662 Bme1580I G_kGCm'C Cuts at: 1790 3723 4879 1790 Size: 1933 1156 2573 Fragments arranged by size: 2573 1933 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3393 4351 442 Size: 416 53 83 399 24 1976 958 1753 Fragments arranged by size: 1976 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4322 1239 Size: 3083 2579 Bpu10I CC'TnA_GC Cuts at: 2351 2695 2700 2351 Size: 344 5 5313 Fragments arranged by size: 5313 344 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3787 4652 4950 3787 Size: 865 298 4499 Fragments arranged by size: 4499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 2935 4325 882 Size: 617 516 920 1390 2219 Fragments arranged by size: 2219 1390 920 617 516 BsaAI yAC'GTr Cuts at: 493 1204 3453 493 Size: 711 2249 2702 Fragments arranged by size: 2702 2249 711 BsaBI GATnn'nnATC Cuts at: 5656 5656 Size: 5662 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3469 3851 4486 275 Size: 53 83 186 936 1936 382 635 1451 Fragments arranged by size: 1936 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4095 4493 4767 4914 5325 4095 Size: 398 274 147 411 4432 Fragments arranged by size: 4432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2508 2538 2622 2652 1178 Size: 30 165 30 1105 30 84 30 4188 Fragments arranged by size: 4188 1105 165 84 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 2944 1186 Size: 1038 720 3904 Fragments arranged by size: 3904 1038 720 BseYI C'CCAG_C Cuts at: 2125 4865 5361 2125 Size: 2740 496 2426 Fragments arranged by size: 2740 2426 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2156 2324 1163 Size: 573 420 168 4501 Fragments arranged by size: 4501 573 420 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 3723 3808 4879 2097 Size: 1626 85 1071 2880 Fragments arranged by size: 2880 1626 1071 85 BsmI GAATG_Cn' Cuts at: 3173 3266 3173 Size: 93 5569 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2981 3723 3808 4879 1391 Size: 399 307 884 742 85 1071 2174 Fragments arranged by size: 2174 1071 884 742 399 307 85 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 2412 3551 5281 2412 Size: 1139 1730 2793 Fragments arranged by size: 2793 1730 1139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2735 3017 844 Size: 1891 282 3489 Fragments arranged by size: 3489 1891 282 BsrBI CCG'CTC Cuts at: 1394 1823 3549 1394 Size: 429 1726 3507 Fragments arranged by size: 3507 1726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 4157 4331 65 Size: 1183 889 2020 174 1396 Fragments arranged by size: 2020 1396 1183 889 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4306 4493 1700 Size: 110 2496 187 2869 Fragments arranged by size: 2869 2496 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 2114 3722 4734 2114 Size: 1608 1012 3042 Fragments arranged by size: 3042 1608 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 2149 2866 1184 Size: 965 717 3980 Fragments arranged by size: 3980 965 717 BstYI r'GATC_y Cuts at: 1972 2688 2827 2992 3359 3744 3761 5213 5299 Size: 716 139 165 367 385 17 1452 86 Cuts at: 5299 5311 5657 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 716 385 367 346 165 139 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2051 2396 2684 2900 2051 Size: 345 288 216 4813 Fragments arranged by size: 4813 345 288 216 BtgI C'CryG_G Cuts at: 513 1065 2004 2325 2436 2475 2745 2808 513 Size: 552 939 321 111 39 270 63 3367 Fragments arranged by size: 3367 939 552 321 270 111 63 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4923 Fragments arranged by size: 4923 683 56 BtsI GCAGTG_nn' Cuts at: 3178 3972 3992 3178 Size: 794 20 4848 Fragments arranged by size: 4848 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5627 DraI TTT'AAA Cuts at: 2966 2966 Size: 5662 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 3109 3998 Size: 54 1425 50 275 18 549 730 889 Cuts at: 3998 5627 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 730 549 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3592 1353 Size: 2239 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3725 4322 5109 5377 1239 Size: 393 2093 597 787 268 1524 Fragments arranged by size: 2093 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2137 2766 2137 Size: 629 5033 EcoRI G'AATT_C Cuts at: 2971 3349 2971 Size: 378 5284 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2104 2846 2104 Size: 742 4920 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 4809 1678 Size: 3131 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2508 2540 4303 4335 4377 Size: 32 135 32 936 32 1763 32 42 Cuts at: 4377 4409 1373 Size: 32 2626 Fragments arranged by size: 2626 1763 936 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3006 3252 669 Size: 988 281 1068 246 3079 Fragments arranged by size: 3079 1068 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2987 2987 Size: 5662 MfeI C'AATT_G Cuts at: 3261 3261 Size: 5662 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4776 4960 1305 Size: 3471 184 2007 Fragments arranged by size: 3471 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 10 Size: 54 1425 343 549 3291 Fragments arranged by size: 3291 1425 549 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2180 2435 2591 2606 2747 3620 Size: 648 981 33 255 156 15 141 873 Cuts at: 3620 3979 4138 518 Size: 359 159 2042 Fragments arranged by size: 2042 981 873 648 359 255 159 156 141 33 15 MspA1I CmG'CkG Cuts at: 1606 2503 2699 3078 3755 4903 5148 1606 Size: 897 196 379 677 1148 245 2120 Fragments arranged by size: 2120 1148 897 677 379 245 196 NcoI C'CATG_G Cuts at: 513 1065 2004 2325 2436 2808 513 Size: 552 939 321 111 372 3367 Fragments arranged by size: 3367 939 552 372 321 111 NdeI CA'TA_TG Cuts at: 387 387 Size: 5662 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5662 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 2434 3020 4565 1132 Size: 97 1205 586 1545 2229 Fragments arranged by size: 2229 1545 1205 586 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5662 PciI A'CATG_T Cuts at: 4561 4561 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 1108 2451 1108 Size: 1343 4319 PfoI T'CCnGG_A Cuts at: 2211 2250 2211 Size: 39 5623 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2622 2654 3731 3763 5244 5276 2622 Size: 32 1077 32 1481 32 3008 Fragments arranged by size: 3008 1481 1077 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2766 2137 Size: 629 5033 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5662 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PstI C_TGCA'G Cuts at: 830 3014 830 Size: 2184 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 PvuII CAG'CTG Cuts at: 2503 2699 2503 Size: 196 5466 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SbfI CC_TGCA'GG Cuts at: 3014 3014 Size: 5662 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SfcI C'TryA_G Cuts at: 826 1045 2893 3010 4145 4826 5017 826 Size: 219 1848 117 1135 681 191 1471 Fragments arranged by size: 1848 1471 1135 681 219 191 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2989 1872 Size: 1117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3766 4667 4929 820 Size: 197 926 1823 901 262 1553 Fragments arranged by size: 1823 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3453 493 Size: 2960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 2004 2194 2325 2436 2490 2626 2808 Size: 552 939 190 131 111 54 136 182 Cuts at: 2808 3101 513 Size: 293 3074 Fragments arranged by size: 3074 939 552 293 190 182 136 131 111 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2082 3689 3706 3859 4044 4489 325 Size: 1255 502 1607 17 153 185 445 1498 Fragments arranged by size: 1607 1498 1255 502 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3908 5620 Size: 274 80 33 51 1443 207 1724 1712 Cuts at: 5620 96 Size: 138 Fragments arranged by size: 1724 1712 1443 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5622 5654 5622 Size: 32 5630 Tth111I GACn'n_nGTC Cuts at: 1316 2522 1316 Size: 1206 4456 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2987 1870 Size: 1117 4545 XmnI GAAnn'nnTTC Cuts at: 3791 3791 Size: 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI