################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MfeI MflI MluI MluNI MscI MseI MslI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tru9I Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 190 GTATAC GT^[AC][GT]AC AccIII 15 TCCGGA T^CCGGA AciI 30 GCGG CCGC(-3/-1) Asp700I 343 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 89 CTCGGG C^[CT]CG[GA]G BanII 84 GAGCCC G[GA]GC[CT]^C BclI 372 TGATCA T^GATCA BlpI 81 GCTGAGC GC^T[ACGT]AGC BmyI 84 GAGCCC G[AGT]GC[ACT]^C Bpu1102I 81 GCTGAGC GC^T[ACGT]AGC BsaBI 373 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 15 TCCGGA [AT]^CCGG[AT] BseAI 15 TCCGGA T^CCGGA BsiYI 164 CCCCTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 164 CCCCTTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 334 GAGAC GTCTC(1/5) BsoBI 89 CTCGGG C^[CT]CG[GA]G Bsp1286I 84 GAGCCC G[AGT]GC[ACT]^C BspEI 15 TCCGGA T^CCGGA BsrFI 96 GCCGGT [GA]^CCGG[CT] BsrI 337 ACTGG ACTGG(1/-1) Bst1107I 190 GTATAC GTA^TAC BstAPI 36 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 18 GGATG GGATG(2/0) BstZ17I 190 GTATAC GTA^TAC CelII 81 GCTGAGC GC^T[ACGT]AGC Cfr10I 96 GCCGGT [GA]^CCGG[CT] DpnII 373 GATC ^GATC DraII 120 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 183 CCATGG C^C[GA][CT]GG EcoO109I 120 GGGGCCT [GA]G^G[ACGT]CC[CT] FbaI 372 TGATCA T^GATCA FokI 18 GGATG GGATG(9/13) HaeIII 122 GGCC GG^CC HphI 319 GGTGA GGTGA(8/7) MamI 373 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 373 GATC ^GATC MboII 114 GAAGA GAAGA(8/7) MroI 15 TCCGGA T^CCGGA MspA1I 40 CAGCTG C[AC]G^C[GT]G NcoI 183 CCATGG C^CATGG NdeI 281 CATATG CA^TATG NdeII 373 GATC ^GATC PstI 34 CTGCAG CTGCA^G PvuII 40 CAGCTG CAG^CTG Sau3AI 373 GATC ^GATC SfcI 34 CTGCAG C^T[GA][CT]AG SspI 309 AATATT AAT^ATT XmnI 343 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 37 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 76 CAGGAGCTG AvaII 72 GGACC G^G[AT]CC 180 GGTCC BpmI 331 CTGGAG CTGGAG(16/14) 338 CTGGAG BssKI 75 CCAGG ^CC[ACGT]GG 316 CCTGG BstNI 75 CCAGG CC^[AT]GG 316 CCTGG DdeI 52 CTGAG C^T[ACGT]AG 82 CTGAG EcoRII 75 CCAGG ^CC[AT]GG 316 CCTGG EcoT14I 166 CCTTGG C^C[AT][AT]GG 183 CCATGG HapII 16 CCGG C^CGG 97 CCGG HpaII 16 CCGG C^CGG 97 CCGG MnlI 88 CCTC CCTC(7/6) 171 GAGG MspI 16 CCGG C^CGG 97 CCGG MvaI 75 CCAGG CC^[AT]GG 316 CCTGG NlaIV 93 GGAGCC GG[ACGT]^[ACGT]CC 120 GGGGCC ScrFI 75 CCAGG CC^[ACGT]GG 316 CCTGG StyI 166 CCTTGG C^C[AT][AT]GG 183 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BsaJI Cfr13I Fnu4HI ItaI MwoI NlaIII RsaI Sau96I TseI Tsp509I