################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccIII AciI AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MfeI MflI MluI MroI MslI MspA1I MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI TspRI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 73 GTAGAC GT^[AC][GT]AC AccII 7 CGCG CG^CG AflIII 985 ACATGT A^C[GA][CT]GT AlwNI 506 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 973 GAGCAC G[AT]GC[AT]^C AvaI 68 CTCGAG C^[CT]CG[GA]G AvaII 160 GGACC G^G[AT]CC BalI 61 TGGCCA TGG^CCA BclI 840 TGATCA T^GATCA BfaI 344 CTAG C^TAG BmyI 973 GAGCAC G[AGT]GC[ACT]^C BpmI 20 CTGGAG CTGGAG(16/14) BsaBI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 23 GAGACC GGTCTC(1/5) BseRI 478 GAGGAG GAGGAG(10/8) BsiHKAI 973 GAGCAC G[AT]GC[AT]^C BsoBI 68 CTCGAG C^[CT]CG[GA]G Bsp1286I 973 GAGCAC G[AGT]GC[ACT]^C BspHI 141 TCATGA T^CATGA BspLU11I 985 ACATGT A^CATGT BspMI 546 ACCTGC ACCTGC(4/8) BsrDI 386 GCAATG GCAATG(2/0) BssKI 19 CCTGG ^CC[ACGT]GG BstNI 19 CCTGG CC^[AT]GG BstUI 7 CGCG CG^CG EaeI 61 TGGCCA [CT]^GGCC[GA] EcoNI 532 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 19 CCTGG ^CC[AT]GG EcoT22I 308 ATGCAT ATGCA^T FbaI 840 TGATCA T^GATCA HapII 742 CCGG C^CGG HpaII 742 CCGG C^CGG HphI 850 GGTGA GGTGA(8/7) MaeI 344 CTAG C^TAG MamI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 61 TGGCCA TGG^CCA MscI 61 TGGCCA TGG^CCA MspI 742 CCGG C^CGG MvaI 19 CCTGG CC^[AT]GG MvnI 7 CGCG CG^CG NruI 6 TCGCGA TCG^CGA NsiI 308 ATGCAT ATGCA^T NspI 985 ACATGT [GA]CATG^[CT] PaeR7I 68 CTCGAG C^TCGAG PstI 548 CTGCAG CTGCA^G RcaI 141 TCATGA T^CATGA ScrFI 19 CCTGG CC^[ACGT]GG SmlI 68 CTCGAG C^T[CT][GA]AG SpeI 343 ACTAGT A^CTAGT XhoI 68 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 271 AAATTT [GA]^AATT[CT] 786 AAATTT ApoI 271 AAATTT [GA]^AATT[CT] 786 AAATTT BsiYI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 532 CCTAAAAGAGG BslI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 532 CCTAAAAGAGG BsmAI 23 GAGAC GTCTC(1/5) 145 GAGAC BstF5I 393 GGATG GGATG(2/0) 699 GGATG Cfr13I 160 GGACC G^G[ACGT]CC 708 GGGCC DpnII 841 GATC ^GATC 937 GATC FokI 393 GGATG GGATG(9/13) 699 GGATG HincII 281 GTCAAC GT[CT]^[GA]AC 409 GTTGAC HindII 281 GTCAAC GT[CT]^[GA]AC 409 GTTGAC MboI 841 GATC ^GATC 937 GATC MseI 230 TTAA T^TAA 515 TTAA NdeII 841 GATC ^GATC 937 GATC PleI 3 GACTC GAGTC(4/5) 528 GAGTC Sau3AI 841 GATC ^GATC 937 GATC Sau96I 160 GGACC G^G[ACGT]CC 708 GGGCC SfaNI 310 GCATC GCATC(5/9) 508 GCATC TaqI 69 TCGA T^CGA 939 TCGA Tru9I 230 TTAA T^TAA 515 TTAA Tsp509I 272 AATT ^AATT 787 AATT TthHB8I 69 TCGA T^CGA 939 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BbvI Cac8I DdeI EarI Fnu4HI HaeIII HinfI ItaI Ksp632I MaeIII MboII MnlI NlaIII SfcI TfiI TseI Tsp45I