################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BamHI BanI BbeI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AocI 76 CCTAAGG CC^T[ACGT]AGG AspHI 418 GAGCTC G[AT]GC[AT]^C AvrII 243 CCTAGG C^CTAGG BanII 418 GAGCTC G[GA]GC[CT]^C BbrPI 592 CACGTG CAC^GTG BciVI 457 GTATCC GTATCC(6/5) BfaI 244 CTAG C^TAG BlnI 243 CCTAGG C^CTAGG BmyI 418 GAGCTC G[AGT]GC[ACT]^C BsaAI 592 CACGTG [CT]AC^GT[GA] BseRI 229 GAGGAG GAGGAG(10/8) BsiHKAI 418 GAGCTC G[AT]GC[AT]^C BsmI 121 GAATGC GAATGC(1/-1) Bsp1286I 418 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 86 TGTACA T^GTACA BspMI 424 ACCTGC ACCTGC(4/8) BsrGI 86 TGTACA T^GTACA BstEII 525 GGTCACC G^GT[ACGT]ACC BstPI 525 GGTCACC G^GT[ACGT]ACC BstXI 218 CCACTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 76 CCTAAGG CC^T[ACGT]AGG Cac8I 613 GCCAGC GC[ACGT]^[ACGT]GC CfoI 540 GCGC GCG^C DpnII 3 GATC ^GATC DraIII 203 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 76 CCTAAGG CC^T[ACGT]AGG EcoO65I 525 GGTCACC G^GT[ACGT]ACC EcoT22I 303 ATGCAT ATGCA^T HhaI 540 GCGC GCG^C HinP1I 540 GCGC G^CGC HindIII 359 AAGCTT A^AGCTT HinfI 345 GACTC G^A[ACGT]TC MaeI 244 CTAG C^TAG MboI 3 GATC ^GATC NdeII 3 GATC ^GATC NsiI 303 ATGCAT ATGCA^T PflMI 550 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 345 GACTC GAGTC(4/5) PmaCI 592 CACGTG CAC^GTG PmlI 592 CACGTG CAC^GTG SacI 418 GAGCTC GAGCT^C Sau3AI 3 GATC ^GATC SexAI 521 ACCAGGT A^CC[AT]GGT SfaNI 689 GCATC GCATC(5/9) SmlI 269 CTCAAG C^T[CT][GA]AG SspBI 86 TGTACA T^GTACA Tsp509I 32 AATT ^AATT Van91I 550 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 503 GGACC G^G[AT]CC 647 GGACC BalI 463 TGGCCA TGG^CCA 611 TGGCCA BpmI 498 CTCCAG CTGGAG(16/14) 226 CTGGAG BsrDI 693 CATTGC GCAATG(2/0) 107 GCAATG Cfr13I 503 GGACC G^G[ACGT]CC 647 GGACC DdeI 77 CTAAG C^T[ACGT]AG 727 CTAAG DraII 502 AGGACCC [GA]G^G[ACGT]CC[CT] 646 AGGACCC EaeI 463 TGGCCA [CT]^GGCC[GA] 611 TGGCCA EcoNI 243 CCTAGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 516 CCTCTACCAGG EcoO109I 502 AGGACCC [GA]G^G[ACGT]CC[CT] 646 AGGACCC EcoT14I 153 CCTTGG C^C[AT][AT]GG 243 CCTAGG MaeII 470 ACGT A^CGT 593 ACGT MboII 5 TCTTC GAAGA(8/7) 757 TCTTC MluNI 463 TGGCCA TGG^CCA 611 TGGCCA MscI 463 TGGCCA TGG^CCA 611 TGGCCA MspA1I 235 CAGCTG C[AC]G^C[GT]G 570 CAGCTG NlaIII 100 CATG CATG^ 145 CATG PpuMI 502 AGGACCC [GA]G^G[AT]CC[CT] 646 AGGACCC PvuII 235 CAGCTG CAG^CTG 570 CAGCTG Sau96I 503 GGACC G^G[ACGT]CC 647 GGACC SfcI 366 CTACAG C^T[GA][CT]AG 434 CTACAG StuI 251 AGGCCT AGG^CCT 513 AGGCCT StyI 153 CCTTGG C^C[AT][AT]GG 243 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI AluI BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MnlI MvaI MwoI NlaIV RsaI ScrFI TseI Tsp45I TspRI