################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BbeI BbrPI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 336 CCGC CCGC(-3/-1) AfaI 333 GTAC GT^AC AflIII 460 ACATGT A^C[GA][CT]GT AocI 646 CCTGAGG CC^T[ACGT]AGG ApaI 251 GGGCCC GGGCC^C Asp700I 564 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 432 GGATCC G^GATCC BanI 358 GGTGCC G^G[CT][GA]CC BanII 251 GGGCCC G[GA]GC[CT]^C BbsI 214 GAAGAC GAAGAC(2/6) BbvCI 469 GCTGAGG CCTCAGC(-5/-2) BclI 344 TGATCA T^GATCA BcnI 254 CCCGG CC^[GC]GG BmyI 251 GGGCCC G[AGT]GC[ACT]^C BpuAI 214 GAAGAC GAAGAC(2/6) BsiYI 78 CCTATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 78 CCTATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 865 GAGAC GTCTC(1/5) BsmBI 865 GAGACG CGTCTC(1/5) BsmI 115 GAATGC GAATGC(1/-1) Bsp1286I 251 GGGCCC G[AGT]GC[ACT]^C BspHI 26 TCATGA T^CATGA BspLU11I 460 ACATGT A^CATGT Bsu36I 646 CCTGAGG CC^T[ACGT]AGG DrdI 511 GACCATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 646 CCTGAGG CC^T[ACGT]AGG FbaI 344 TGATCA T^GATCA HapII 255 CCGG C^CGG HindIII 565 AAGCTT A^AGCTT HpaII 255 CCGG C^CGG HphI 440 TCACC GGTGA(8/7) MfeI 110 CAATTG C^AATTG MseI 781 TTAA T^TAA MslI 303 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 255 CCGG C^CGG MunI 110 CAATTG C^AATTG MwoI 341 GCATGATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 254 CCCGG CC^[GC]GG NspI 460 ACATGT [GA]CATG^[CT] PleI 714 GACTC GAGTC(4/5) PpuMI 74 AGGACCT [GA]G^G[AT]CC[CT] PvuII 662 CAGCTG CAG^CTG RcaI 26 TCATGA T^CATGA RsaI 333 GTAC GT^AC SfcI 240 CTACAG C^T[GA][CT]AG TfiI 593 GAATC G^A[AT]TC Tru9I 781 TTAA T^TAA XmnI 564 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 121 AAATTC [GA]^AATT[CT] 419 GAATTT ApoI 121 AAATTC [GA]^AATT[CT] 419 GAATTT AvaII 75 GGACC G^G[AT]CC 267 GGACC BseRI 559 GAGGAG GAGGAG(10/8) 862 GAGGAG BstNI 147 CCTGG CC^[AT]GG 279 CCTGG BstYI 432 GGATCC [GA]^GATC[CT] 642 AGATCC DraII 74 AGGACCT [GA]G^G[ACGT]CC[CT] 250 AGGGCCC EcoO109I 74 AGGACCT [GA]G^G[ACGT]CC[CT] 250 AGGGCCC EcoRII 147 CCTGG ^CC[AT]GG 279 CCTGG HaeIII 252 GGCC GG^CC 486 GGCC HinfI 593 GAATC G^A[ACGT]TC 714 GACTC MboII 214 GAAGA GAAGA(8/7) 773 TCTTC MflI 432 GGATCC [GA]^GATC[CT] 642 AGATCC MspA1I 336 CCGCTG C[AC]G^C[GT]G 662 CAGCTG MvaI 147 CCTGG CC^[AT]GG 279 CCTGG SfaNI 322 GATGC GCATC(5/9) 818 GCATC XhoII 432 GGATCC [GA]^GATC[CT] 642 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsgI BsrI BssKI BstF5I Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI ItaI MaeIII MboI MnlI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI Tsp45I Tsp509I TspRI