(Linear) (Six-base) MAPSORT of: KIEE5672.seq Check: 8284 from: 1 to: 1635 KIEE5672.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1218 1635 Size: 1218 417 AccI GT'mk_AC Cuts at: 0 482 1286 1522 1635 Size: 482 804 236 113 Fragments arranged by size: 804 482 236 113 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1027 1528 1635 Size: 1027 501 107 Fragments arranged by size: 1027 501 107 AleI CACnn'nnGTG Cuts at: 0 805 1635 Size: 805 830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 823 1635 Size: 823 812 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 742 774 1635 Size: 742 32 861 Fragments arranged by size: 861 742 32 AlwNI CAG_nnn'CTG Cuts at: 0 44 1050 1635 Size: 44 1006 585 Fragments arranged by size: 1006 585 44 ApaI G_GGCC'C Cuts at: 0 265 1635 Size: 265 1370 AscI GG'CGCG_CC Cuts at: 0 1171 1635 Size: 1171 464 AvaI C'yCGr_G Cuts at: 0 256 864 943 1054 1635 Size: 256 608 79 111 581 Fragments arranged by size: 608 581 256 111 79 AvrII C'CTAG_G Cuts at: 0 366 1635 Size: 366 1269 BanI G'GyrC_C Cuts at: 0 100 728 1635 Size: 100 628 907 Fragments arranged by size: 907 628 100 BanII G_rGCy'C Cuts at: 0 265 1635 Size: 265 1370 BbeI G_GCGC'C Cuts at: 0 732 1635 Size: 732 903 BbsI GAAGACnn'nnnn_ Cuts at: 0 176 182 462 1635 Size: 176 6 280 1173 Fragments arranged by size: 1173 280 176 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 588 622 1635 Size: 588 34 1013 Fragments arranged by size: 1013 588 34 BglI GCCn_nnn'nGGC Cuts at: 0 778 1635 Size: 778 857 BlpI GC'TnA_GC Cuts at: 0 406 628 1635 Size: 406 222 1007 Fragments arranged by size: 1007 406 222 Bme1580I G_kGCm'C Cuts at: 0 105 265 1310 1635 Size: 105 160 1045 325 Fragments arranged by size: 1045 325 160 105 BmrI ACTGGGnnnn_n' Cuts at: 0 570 1635 Size: 570 1065 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 565 780 925 1635 Size: 51 514 215 145 710 Fragments arranged by size: 710 514 215 145 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 559 625 1147 1635 Size: 559 66 522 488 Fragments arranged by size: 559 522 488 66 BsaI GGTCTCn'nnnn_ Cuts at: 0 1390 1635 Size: 1390 245 BsaAI yAC'GTr Cuts at: 0 912 1635 Size: 912 723 BsaHI Gr'CG_yC Cuts at: 0 729 1635 Size: 729 906 BsaWI w'CCGG_w Cuts at: 0 1183 1635 Size: 1183 452 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 181 211 742 772 907 937 1635 Size: 181 30 531 30 135 30 698 Fragments arranged by size: 698 531 181 135 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 33 199 202 442 562 625 694 753 Size: 33 166 3 240 120 63 69 59 Cuts at: 753 862 1635 Size: 109 773 Fragments arranged by size: 773 240 166 120 109 69 63 59 33 3 BseYI C'CCAG_C Cuts at: 0 265 552 1309 1635 Size: 265 287 757 326 Fragments arranged by size: 757 326 287 265 BsiEI CG_ry'CG Cuts at: 0 456 467 1635 Size: 456 11 1168 Fragments arranged by size: 1168 456 11 BsiHKAI G_wGCw'C Cuts at: 0 518 812 911 1301 1635 Size: 518 294 99 390 334 Fragments arranged by size: 518 390 334 294 99 BsmBI CGTCTCn'nnnn_ Cuts at: 0 15 819 1635 Size: 15 804 816 Fragments arranged by size: 816 804 15 Bsp1286I G_dGCh'C Cuts at: 0 105 265 518 812 911 1301 1310 1635 Size: 105 160 253 294 99 390 9 325 Fragments arranged by size: 390 325 294 253 160 105 99 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 663 899 959 1218 1400 1635 Size: 663 236 60 259 182 235 Fragments arranged by size: 663 259 236 235 182 60 BsrBI CCG'CTC Cuts at: 0 615 1035 1635 Size: 615 420 600 Fragments arranged by size: 615 600 420 BsrDI GCAATG_nn' Cuts at: 0 1430 1635 Size: 1430 205 BsrFI r'CCGG_y Cuts at: 0 346 1635 Size: 346 1289 BssHII G'CGCG_C Cuts at: 0 660 662 991 1171 1635 Size: 660 2 329 180 464 Fragments arranged by size: 660 464 329 180 2 BssSI C'ACGA_G Cuts at: 0 652 1635 Size: 652 983 BstAPI GCAn_nnn'nTGC Cuts at: 0 657 1305 1635 Size: 657 648 330 Fragments arranged by size: 657 648 330 BstEII G'GTnAC_C Cuts at: 0 240 1635 Size: 240 1395 BstXI CCAn_nnnn'nTGG Cuts at: 0 789 1635 Size: 789 846 BstYI r'GATC_y Cuts at: 0 644 1635 Size: 644 991 BstZ17I GTA'TAC Cuts at: 0 1523 1635 Size: 1523 112 Bsu36I CC'TnA_GG Cuts at: 0 979 1635 Size: 979 656 BtgI C'CryG_G Cuts at: 0 129 818 842 1256 1352 1370 1635 Size: 129 689 24 414 96 18 265 Fragments arranged by size: 689 414 265 129 96 24 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1236 1635 Size: 1236 399 DraIII CAC_nnn'GTG Cuts at: 0 1305 1635 Size: 1305 330 DrdI GACnn_nn'nnGTC Cuts at: 0 466 1635 Size: 466 1169 EaeI y'GGCC_r Cuts at: 0 312 344 377 464 770 1022 1086 1635 Size: 312 32 33 87 306 252 64 549 Fragments arranged by size: 549 312 306 252 87 64 33 32 EagI C'GGCC_G Cuts at: 0 464 1635 Size: 464 1171 EarI CTCTTCn'nnn_ Cuts at: 0 212 700 1252 1635 Size: 212 488 552 383 Fragments arranged by size: 552 488 383 212 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 700 1635 Size: 700 935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 565 780 925 1027 1528 1635 Size: 51 514 215 145 102 501 107 Fragments arranged by size: 514 501 215 145 107 102 51 EcoO109I rG'GnC_Cy Cuts at: 0 41 261 262 1635 Size: 41 220 1 1373 Fragments arranged by size: 1373 220 41 1 FspI TGC'GCA Cuts at: 0 1147 1635 Size: 1147 488 FspAI rTGC'GCAy Cuts at: 0 1147 1635 Size: 1147 488 HaeII r_GCGC'y Cuts at: 0 732 829 835 987 1001 1635 Size: 732 97 6 152 14 634 Fragments arranged by size: 732 634 152 97 14 6 KasI G'GCGC_C Cuts at: 0 728 1635 Size: 728 907 MscI TGG'CCA Cuts at: 0 314 379 1024 1635 Size: 314 65 645 611 Fragments arranged by size: 645 611 314 65 MslI CAynn'nnrTG Cuts at: 0 787 805 1255 1635 Size: 787 18 450 380 Fragments arranged by size: 787 450 380 18 MspA1I CmG'CkG Cuts at: 0 327 477 726 1047 1354 1635 Size: 327 150 249 321 307 281 Fragments arranged by size: 327 321 307 281 249 150 NaeI GCC'GGC Cuts at: 0 348 1635 Size: 348 1287 NarI GG'CG_CC Cuts at: 0 729 1635 Size: 729 906 NcoI C'CATG_G Cuts at: 0 1256 1635 Size: 1256 379 NgoMIV G'CCGG_C Cuts at: 0 346 1635 Size: 346 1289 NspI r_CATG'y Cuts at: 0 708 1635 Size: 708 927 PasI CC'CwG_GG Cuts at: 0 96 1635 Size: 96 1539 PflMI CCAn_nnn'nTGG Cuts at: 0 1095 1230 1635 Size: 1095 135 405 Fragments arranged by size: 1095 405 135 PfoI T'CCnGG_A Cuts at: 0 28 542 902 1635 Size: 28 514 360 733 Fragments arranged by size: 733 514 360 28 PmlI CAC'GTG Cuts at: 0 912 1635 Size: 912 723 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 742 774 1635 Size: 742 32 861 Fragments arranged by size: 861 742 32 PspOMI G'GGCC_C Cuts at: 0 261 1635 Size: 261 1374 PspXI vC'TCGA_Gb Cuts at: 0 943 1054 1635 Size: 943 111 581 Fragments arranged by size: 943 581 111 PvuII CAG'CTG Cuts at: 0 327 477 1047 1635 Size: 327 150 570 588 Fragments arranged by size: 588 570 327 150 SacII CC_GC'GG Cuts at: 0 1355 1635 Size: 1355 280 SapI GCTCTTCn'nnn_ Cuts at: 0 1252 1635 Size: 1252 383 SexAI A'CCwGG_T Cuts at: 0 1007 1016 1635 Size: 1007 9 619 Fragments arranged by size: 1007 619 9 SfcI C'TryA_G Cuts at: 0 36 1497 1635 Size: 36 1461 138 Fragments arranged by size: 1461 138 36 SfiI GGCCn_nnn'nGGCC Cuts at: 0 778 1635 Size: 778 857 SfoI GGC'GCC Cuts at: 0 730 1635 Size: 730 905 SmlI C'TyrA_G Cuts at: 0 574 640 943 1054 1162 1635 Size: 574 66 303 111 108 473 Fragments arranged by size: 574 473 303 111 108 66 StyI C'CwwG_G Cuts at: 0 366 776 1256 1635 Size: 366 410 480 379 Fragments arranged by size: 480 410 379 366 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 88 298 1635 Size: 88 210 1337 Fragments arranged by size: 1337 210 88 TatI w'GTAC_w Cuts at: 0 578 1635 Size: 578 1057 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 181 213 907 939 1635 Size: 181 32 694 32 696 Fragments arranged by size: 696 694 181 32 32 XhoI C'TCGA_G Cuts at: 0 943 1054 1635 Size: 943 111 581 Fragments arranged by size: 943 581 111 XmnI GAAnn'nnTTC Cuts at: 0 757 1635 Size: 757 878 Enzymes that do cut: AarI AccI AcuI AleI AlfI AloI AlwNI ApaI AscI AvaI AvrII BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FspI FspAI HaeII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PspOMI PspXI PvuII SacII SapI SexAI SfcI SfiI SfoI SmlI StyI TaqII TatI TstI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI ApaLI ApoI AseI AsiSI BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaBI BsgI BsiWI BsmI BspEI BspHI BsrGI BstBI BtsI ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpuMI PshAI PsiI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XmaI ZraI