(Linear) (Six-base) MAPSORT of: KIEE1178.seq Check: 2071 from: 1 to: 2058 KIEE1178.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 608 759 1011 1095 1500 1584 1668 1752 Size: 608 151 252 84 405 84 84 84 Cuts at: 1752 2058 Size: 306 Fragments arranged by size: 608 405 306 252 151 84 84 84 84 AleI CACnn'nnGTG Cuts at: 0 113 1861 2058 Size: 113 1748 197 Fragments arranged by size: 1748 197 113 AlwNI CAG_nnn'CTG Cuts at: 0 60 782 2058 Size: 60 722 1276 Fragments arranged by size: 1276 722 60 ApaI G_GGCC'C Cuts at: 0 81 1818 2058 Size: 81 1737 240 Fragments arranged by size: 1737 240 81 ApaLI G'TGCA_C Cuts at: 0 31 2058 Size: 31 2027 ApoI r'AATT_y Cuts at: 0 1169 1214 1961 2045 2058 Size: 1169 45 747 84 13 Fragments arranged by size: 1169 747 84 45 13 AseI AT'TA_AT Cuts at: 0 650 2058 Size: 650 1408 AvaI C'yCGr_G Cuts at: 0 41 415 2058 Size: 41 374 1643 Fragments arranged by size: 1643 374 41 BanI G'GyrC_C Cuts at: 0 225 2058 Size: 225 1833 BanII G_rGCy'C Cuts at: 0 81 871 955 1123 1444 1780 1818 2058 Size: 81 790 84 168 321 336 38 240 Fragments arranged by size: 790 336 321 240 168 84 81 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 765 1916 2058 Size: 765 1151 142 Fragments arranged by size: 1151 765 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 22 56 2058 Size: 22 34 2002 Fragments arranged by size: 2002 34 22 BfrBI ATG'CAT Cuts at: 0 1149 2058 Size: 1149 909 Bme1580I G_kGCm'C Cuts at: 0 35 81 1818 2058 Size: 35 46 1737 240 Fragments arranged by size: 1737 240 46 35 BmrI ACTGGGnnnn_n' Cuts at: 0 911 1079 1163 1812 2058 Size: 911 168 84 649 246 Fragments arranged by size: 911 649 246 168 84 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 177 2058 Size: 177 1881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 190 1328 1412 1496 1664 2058 Size: 121 69 1138 84 84 168 394 Fragments arranged by size: 1138 394 168 121 84 84 69 Bpu10I CC'TnA_GC Cuts at: 0 152 865 2058 Size: 152 713 1193 Fragments arranged by size: 1193 713 152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 939 2007 2058 Size: 939 1068 51 Fragments arranged by size: 1068 939 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 40 70 2058 Size: 40 30 1988 Fragments arranged by size: 1988 40 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 478 610 2058 Size: 61 417 132 1448 Fragments arranged by size: 1448 417 132 61 BseYI C'CCAG_C Cuts at: 0 519 1133 2033 2058 Size: 519 614 900 25 Fragments arranged by size: 900 614 519 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 776 1767 2058 Size: 776 991 291 Fragments arranged by size: 991 776 291 BsiHKAI G_wGCw'C Cuts at: 0 35 871 955 1123 1612 1780 2058 Size: 35 836 84 168 489 168 278 Fragments arranged by size: 836 489 278 168 168 84 35 BsiWI C'GTAC_G Cuts at: 0 746 2058 Size: 746 1312 BsmI GAATG_Cn' Cuts at: 0 685 1582 1845 2058 Size: 685 897 263 213 Fragments arranged by size: 897 685 263 213 BsmBI CGTCTCn'nnnn_ Cuts at: 0 362 2058 Size: 362 1696 Bsp1286I G_dGCh'C Cuts at: 0 35 81 871 955 1123 1444 1612 1780 Size: 35 46 790 84 168 321 168 168 Cuts at: 1780 1818 2058 Size: 38 240 Fragments arranged by size: 790 321 240 168 168 168 84 46 38 35 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 209 2058 Size: 209 1849 BsrDI GCAATG_nn' Cuts at: 0 1998 2058 Size: 1998 60 BsrGI T'GTAC_A Cuts at: 0 1336 2058 Size: 1336 722 BstYI r'GATC_y Cuts at: 0 1271 2058 Size: 1271 787 BtgI C'CryG_G Cuts at: 0 187 732 2058 Size: 187 545 1326 Fragments arranged by size: 1326 545 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 35 2058 Size: 35 2023 BtsI GCAGTG_nn' Cuts at: 0 61 120 1755 2058 Size: 61 59 1635 303 Fragments arranged by size: 1635 303 61 59 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1080 1115 1980 2015 2058 Size: 1080 35 865 35 43 Fragments arranged by size: 1080 865 43 35 35 DraI TTT'AAA Cuts at: 0 834 1407 2058 Size: 834 573 651 Fragments arranged by size: 834 651 573 DraIII CAC_nnn'GTG Cuts at: 0 845 2058 Size: 845 1213 EaeI y'GGCC_r Cuts at: 0 69 2058 Size: 69 1989 EarI CTCTTCn'nnn_ Cuts at: 0 176 2058 Size: 176 1882 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 850 2058 Size: 850 1208 EcoICRI GAG'CTC Cuts at: 0 869 953 1121 1778 2058 Size: 869 84 168 657 280 Fragments arranged by size: 869 657 280 168 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 190 608 759 1011 1095 1328 1412 Size: 121 69 418 151 252 84 233 84 Cuts at: 1412 1496 1500 1584 1664 1668 1752 2058 Size: 84 4 84 80 4 84 306 Fragments arranged by size: 418 306 252 233 151 121 84 84 84 84 84 80 69 4 4 EcoO109I rG'GnC_Cy Cuts at: 0 77 78 192 233 995 1079 1652 1814 Size: 77 1 114 41 762 84 573 162 Cuts at: 1814 2058 Size: 244 Fragments arranged by size: 762 573 244 162 114 84 77 41 1 EcoRI G'AATT_C Cuts at: 0 1214 1961 2058 Size: 1214 747 97 Fragments arranged by size: 1214 747 97 EcoRV GAT'ATC Cuts at: 0 630 2058 Size: 630 1428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1275 1307 1767 1799 2058 Size: 1275 32 460 32 259 Fragments arranged by size: 1275 460 259 32 32 HindIII A'AGCT_T Cuts at: 0 938 1259 2006 2058 Size: 938 321 747 52 Fragments arranged by size: 938 747 321 52 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1731 2058 Size: 1731 327 MscI TGG'CCA Cuts at: 0 71 2058 Size: 71 1987 MslI CAynn'nnrTG Cuts at: 0 113 1239 1407 1861 1904 2058 Size: 113 1126 168 454 43 154 Fragments arranged by size: 1126 454 168 154 113 43 MspA1I CmG'CkG Cuts at: 0 530 1044 1296 2058 Size: 530 514 252 762 Fragments arranged by size: 762 530 514 252 NcoI C'CATG_G Cuts at: 0 187 732 2058 Size: 187 545 1326 Fragments arranged by size: 1326 545 187 NsiI A_TGCA'T Cuts at: 0 1151 2058 Size: 1151 907 PasI CC'CwG_GG Cuts at: 0 504 2058 Size: 504 1554 PpuMI rG'GwC_Cy Cuts at: 0 192 233 2058 Size: 192 41 1825 Fragments arranged by size: 1825 192 41 PshAI GACnn'nnGTC Cuts at: 0 322 2058 Size: 322 1736 PsiI TTA'TAA Cuts at: 0 1322 1985 2058 Size: 1322 663 73 Fragments arranged by size: 1322 663 73 PspOMI G'GGCC_C Cuts at: 0 77 1814 2058 Size: 77 1737 244 Fragments arranged by size: 1737 244 77 PstI C_TGCA'G Cuts at: 0 206 2058 Size: 206 1852 PvuII CAG'CTG Cuts at: 0 1044 1296 2058 Size: 1044 252 762 Fragments arranged by size: 1044 762 252 SacI G_AGCT'C Cuts at: 0 871 955 1123 1780 2058 Size: 871 84 168 657 278 Fragments arranged by size: 871 657 278 168 84 SapI GCTCTTCn'nnn_ Cuts at: 0 176 2058 Size: 176 1882 SbfI CC_TGCA'GG Cuts at: 0 206 2058 Size: 206 1852 SfcI C'TryA_G Cuts at: 0 84 202 915 1223 2058 Size: 84 118 713 308 835 Fragments arranged by size: 835 713 308 118 84 SmaI CCC'GGG Cuts at: 0 417 2058 Size: 417 1641 SmlI C'TyrA_G Cuts at: 0 954 2022 2058 Size: 954 1068 36 Fragments arranged by size: 1068 954 36 StuI AGG'CCT Cuts at: 0 277 1108 2058 Size: 277 831 950 Fragments arranged by size: 950 831 277 StyI C'CwwG_G Cuts at: 0 187 229 491 732 2058 Size: 187 42 262 241 1326 Fragments arranged by size: 1326 262 241 187 42 SwaI ATTT'AAAT Cuts at: 0 1407 2058 Size: 1407 651 TatI w'GTAC_w Cuts at: 0 1336 2058 Size: 1336 722 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 40 72 2058 Size: 40 32 1986 Fragments arranged by size: 1986 40 32 XmaI C'CCGG_G Cuts at: 0 415 2058 Size: 415 1643 XmnI GAAnn'nnTTC Cuts at: 0 772 1024 1108 1681 2058 Size: 772 252 84 573 377 Fragments arranged by size: 772 573 377 252 84 Enzymes that do cut: AcuI AleI AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII BbsI BcgI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BstYI BtgI BtgZI BtsI CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HindIII MmeI MscI MslI MspA1I NcoI NsiI PasI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SapI SbfI SfcI SmaI SmlI StuI StyI SwaI TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BglI BglII BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI DrdI EagI EcoNI FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI TaqII Tth111I XbaI XcmI XhoI ZraI