(Linear) (Six-base) MAPSORT of: KIEE9520.seq Check: 6051 from: 1 to: 1335 KIEE9520.orf With 182 enzymes: SgfI * March 22, 2012 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 47 243 422 1335 Size: 47 196 179 913 Fragments arranged by size: 913 196 179 47 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 602 1335 Size: 602 733 AflIII A'CryG_T Cuts at: 0 1292 1335 Size: 1292 43 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 295 387 1335 Size: 295 92 948 Fragments arranged by size: 948 295 92 AlwNI CAG_nnn'CTG Cuts at: 0 258 625 830 1335 Size: 258 367 205 505 Fragments arranged by size: 505 367 258 205 AvaI C'yCGr_G Cuts at: 0 508 1335 Size: 508 827 BanI G'GyrC_C Cuts at: 0 296 535 775 1099 1335 Size: 296 239 240 324 236 Fragments arranged by size: 324 296 240 239 236 BanII G_rGCy'C Cuts at: 0 67 711 1081 1246 1335 Size: 67 644 370 165 89 Fragments arranged by size: 644 370 165 89 67 BbsI GAAGACnn'nnnn_ Cuts at: 0 631 1335 Size: 631 704 BglI GCCn_nnn'nGGC Cuts at: 0 283 313 839 1228 1335 Size: 283 30 526 389 107 Fragments arranged by size: 526 389 283 107 30 Bme1580I G_kGCm'C Cuts at: 0 274 465 517 597 1102 1335 Size: 274 191 52 80 505 233 Fragments arranged by size: 505 274 233 191 80 52 BmrI ACTGGGnnnn_n' Cuts at: 0 304 1335 Size: 304 1031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 222 682 1335 Size: 222 460 653 Fragments arranged by size: 653 460 222 Bpu10I CC'TnA_GC Cuts at: 0 1075 1335 Size: 1075 260 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 426 583 1335 Size: 426 157 752 Fragments arranged by size: 752 426 157 BsaAI yAC'GTr Cuts at: 0 1164 1293 1335 Size: 1164 129 42 Fragments arranged by size: 1164 129 42 BsaHI Gr'CG_yC Cuts at: 0 918 1335 Size: 918 417 BsaWI w'CCGG_w Cuts at: 0 24 1335 Size: 24 1311 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 219 1335 Size: 219 1116 BseYI C'CCAG_C Cuts at: 0 258 837 945 1014 1032 1335 Size: 258 579 108 69 18 303 Fragments arranged by size: 579 303 258 108 69 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 511 910 1017 1335 Size: 271 240 399 107 318 Fragments arranged by size: 399 318 271 240 107 BsiEI CG_ry'CG Cuts at: 0 1207 1335 Size: 1207 128 BsmI GAATG_Cn' Cuts at: 0 327 1335 Size: 327 1008 BsmBI CGTCTCn'nnnn_ Cuts at: 0 156 1335 Size: 156 1179 Bsp1286I G_dGCh'C Cuts at: 0 67 274 465 517 597 711 1081 1102 Size: 67 207 191 52 80 114 370 21 Cuts at: 1102 1246 1335 Size: 144 89 Fragments arranged by size: 370 207 191 144 114 89 80 67 52 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 47 222 243 422 989 1335 Size: 47 175 21 179 567 346 Fragments arranged by size: 567 346 179 175 47 21 BsrBI CCG'CTC Cuts at: 0 552 1335 Size: 552 783 BsrDI GCAATG_nn' Cuts at: 0 1210 1335 Size: 1210 125 BsrFI r'CCGG_y Cuts at: 0 1202 1206 1222 1335 Size: 1202 4 16 113 Fragments arranged by size: 1202 113 16 4 BssSI C'ACGA_G Cuts at: 0 1120 1335 Size: 1120 215 BstAPI GCAn_nnn'nTGC Cuts at: 0 376 601 830 1335 Size: 376 225 229 505 Fragments arranged by size: 505 376 229 225 BstEII G'GTnAC_C Cuts at: 0 192 1335 Size: 192 1143 BstXI CCAn_nnnn'nTGG Cuts at: 0 131 246 966 1239 1335 Size: 131 115 720 273 96 Fragments arranged by size: 720 273 131 115 96 BstYI r'GATC_y Cuts at: 0 86 1335 Size: 86 1249 Bsu36I CC'TnA_GG Cuts at: 0 797 1335 Size: 797 538 BtgI C'CryG_G Cuts at: 0 983 1335 Size: 983 352 BtsI GCAGTG_nn' Cuts at: 0 745 831 957 1335 Size: 745 86 126 378 Fragments arranged by size: 745 378 126 86 DraIII CAC_nnn'GTG Cuts at: 0 303 1335 Size: 303 1032 DrdI GACnn_nn'nnGTC Cuts at: 0 232 1335 Size: 232 1103 EaeI y'GGCC_r Cuts at: 0 848 980 1204 1229 1335 Size: 848 132 224 25 106 Fragments arranged by size: 848 224 132 106 25 EagI C'GGCC_G Cuts at: 0 1204 1335 Size: 1204 131 EarI CTCTTCn'nnn_ Cuts at: 0 58 719 728 1335 Size: 58 661 9 607 Fragments arranged by size: 661 607 58 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 222 602 682 1335 Size: 222 380 80 653 Fragments arranged by size: 653 380 222 80 EcoNI CCTnn'n_nnAGG Cuts at: 0 490 1335 Size: 490 845 EcoO109I rG'GnC_Cy Cuts at: 0 234 1001 1335 Size: 234 767 334 Fragments arranged by size: 767 334 234 FseI GG_CCGG'CC Cuts at: 0 1206 1335 Size: 1206 129 HaeII r_GCGC'y Cuts at: 0 1066 1259 1335 Size: 1066 193 76 Fragments arranged by size: 1066 193 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 673 680 705 712 1335 Size: 673 7 25 7 623 Fragments arranged by size: 673 623 25 7 7 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 112 1335 Size: 112 1223 MscI TGG'CCA Cuts at: 0 982 1231 1335 Size: 982 249 104 Fragments arranged by size: 982 249 104 MslI CAynn'nnrTG Cuts at: 0 244 369 988 1237 1335 Size: 244 125 619 249 98 Fragments arranged by size: 619 249 244 125 98 MspA1I CmG'CkG Cuts at: 0 622 1335 Size: 622 713 NaeI GCC'GGC Cuts at: 0 1204 1208 1224 1335 Size: 1204 4 16 111 Fragments arranged by size: 1204 111 16 4 NcoI C'CATG_G Cuts at: 0 983 1335 Size: 983 352 NgoMIV G'CCGG_C Cuts at: 0 1202 1206 1222 1335 Size: 1202 4 16 113 Fragments arranged by size: 1202 113 16 4 NspI r_CATG'y Cuts at: 0 374 1335 Size: 374 961 PflMI CCAn_nnn'nTGG Cuts at: 0 42 1335 Size: 42 1293 PmlI CAC'GTG Cuts at: 0 1293 1335 Size: 1293 42 PpuMI rG'GwC_Cy Cuts at: 0 234 1001 1335 Size: 234 767 334 Fragments arranged by size: 767 334 234 PspXI vC'TCGA_Gb Cuts at: 0 508 1335 Size: 508 827 PvuII CAG'CTG Cuts at: 0 622 1335 Size: 622 713 SmlI C'TyrA_G Cuts at: 0 405 508 562 1335 Size: 405 103 54 773 Fragments arranged by size: 773 405 103 54 SphI G_CATG'C Cuts at: 0 374 1335 Size: 374 961 StuI AGG'CCT Cuts at: 0 455 695 802 1335 Size: 455 240 107 533 Fragments arranged by size: 533 455 240 107 StyI C'CwwG_G Cuts at: 0 983 1004 1335 Size: 983 21 331 Fragments arranged by size: 983 331 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 24 81 976 1125 1335 Size: 24 57 895 149 210 Fragments arranged by size: 895 210 149 57 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 771 803 1335 Size: 771 32 532 Fragments arranged by size: 771 532 32 XhoI C'TCGA_G Cuts at: 0 508 1335 Size: 508 827 Enzymes that do cut: AarI AcuI AflIII AlfI AlwNI AvaI BanI BanII BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PmlI PpuMI PspXI PvuII SmlI SphI StuI StyI TaqII TstI XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaBI BsaXI BsiHKAI BsiWI BspEI BspHI BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI EciI EcoICRI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XcmI XmaI XmnI ZraI