(Linear) (Six-base) MAPSORT of: KIEE4269.seq Check: 5949 from: 1 to: 3387 KIEE4269.orf With 182 enzymes: SgfI * March 20, 2014 14:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1123 3387 Size: 1123 2264 Acc65I G'GTAC_C Cuts at: 0 986 3387 Size: 986 2401 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 3076 3143 3387 Size: 196 2880 67 244 Fragments arranged by size: 2880 244 196 67 AfeI AGC'GCT Cuts at: 0 2194 3387 Size: 2194 1193 AflIII A'CryG_T Cuts at: 0 2097 3387 Size: 2097 1290 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1023 1055 1653 1685 1901 1933 3387 Size: 1023 32 598 32 216 32 1454 Fragments arranged by size: 1454 1023 598 216 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 601 3387 Size: 601 2786 ApaI G_GGCC'C Cuts at: 0 2546 3387 Size: 2546 841 ApoI r'AATT_y Cuts at: 0 1563 3387 Size: 1563 1824 AvrII C'CTAG_G Cuts at: 0 2383 3387 Size: 2383 1004 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3149 3182 3387 Size: 3149 33 205 Fragments arranged by size: 3149 205 33 BamHI G'GATC_C Cuts at: 0 1010 3387 Size: 1010 2377 BanI G'GyrC_C Cuts at: 0 343 632 986 3387 Size: 343 289 354 2401 Fragments arranged by size: 2401 354 343 289 BanII G_rGCy'C Cuts at: 0 2546 3257 3387 Size: 2546 711 130 Fragments arranged by size: 2546 711 130 BbeI G_GCGC'C Cuts at: 0 636 3387 Size: 636 2751 BbsI GAAGACnn'nnnn_ Cuts at: 0 1145 1310 1669 3131 3223 3387 Size: 1145 165 359 1462 92 164 Fragments arranged by size: 1462 1145 359 165 164 92 BciVI GTATCCnnnnn_n' Cuts at: 0 925 3387 Size: 925 2462 BfrBI ATG'CAT Cuts at: 0 942 3387 Size: 942 2445 BglII A'GATC_T Cuts at: 0 4 71 480 2058 2318 3387 Size: 4 67 409 1578 260 1069 Fragments arranged by size: 1578 1069 409 260 67 4 BlpI GC'TnA_GC Cuts at: 0 3032 3387 Size: 3032 355 Bme1580I G_kGCm'C Cuts at: 0 2546 3387 Size: 2546 841 BmgBI CAC'GTC Cuts at: 0 128 2100 3387 Size: 128 1972 1287 Fragments arranged by size: 1972 1287 128 BmrI ACTGGGnnnn_n' Cuts at: 0 2043 3387 Size: 2043 1344 BmtI G_CTAG'C Cuts at: 0 2998 3387 Size: 2998 389 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2498 3387 Size: 2498 889 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 199 391 934 2027 2211 2828 3032 Size: 9 190 192 543 1093 184 617 204 Cuts at: 3032 3387 Size: 355 Fragments arranged by size: 1093 617 543 355 204 192 190 184 9 Bpu10I CC'TnA_GC Cuts at: 0 1969 2870 3387 Size: 1969 901 517 Fragments arranged by size: 1969 901 517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 469 1723 3387 Size: 469 1254 1664 Fragments arranged by size: 1664 1254 469 BsaI GGTCTCn'nnnn_ Cuts at: 0 91 452 2804 2981 3387 Size: 91 361 2352 177 406 Fragments arranged by size: 2352 406 361 177 91 BsaAI yAC'GTr Cuts at: 0 2106 3387 Size: 2106 1281 BsaBI GATnn'nnATC Cuts at: 0 2156 3387 Size: 2156 1231 BsaHI Gr'CG_yC Cuts at: 0 633 3101 3160 3387 Size: 633 2468 59 227 Fragments arranged by size: 2468 633 227 59 BsaWI w'CCGG_w Cuts at: 0 1013 3387 Size: 1013 2374 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1023 1053 2352 2382 3387 Size: 1023 30 1299 30 1005 Fragments arranged by size: 1299 1023 1005 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 196 1044 2149 2214 2471 3387 Size: 196 848 1105 65 257 916 Fragments arranged by size: 1105 916 848 257 196 65 BseYI C'CCAG_C Cuts at: 0 1570 2524 2630 2901 2982 3387 Size: 1570 954 106 271 81 405 Fragments arranged by size: 1570 954 405 271 106 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 845 3109 3387 Size: 133 712 2264 278 Fragments arranged by size: 2264 712 278 133 BsiHKAI G_wGCw'C Cuts at: 0 560 2644 3257 3387 Size: 560 2084 613 130 Fragments arranged by size: 2084 613 560 130 BsmI GAATG_Cn' Cuts at: 0 1469 3387 Size: 1469 1918 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3092 3151 3387 Size: 3092 59 236 Fragments arranged by size: 3092 236 59 Bsp1286I G_dGCh'C Cuts at: 0 560 2546 2644 3257 3387 Size: 560 1986 98 613 130 Fragments arranged by size: 1986 613 560 130 98 BspEI T'CCGG_A Cuts at: 0 1013 3387 Size: 1013 2374 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 998 1123 2822 2918 2972 2999 3387 Size: 998 125 1699 96 54 27 388 Fragments arranged by size: 1699 998 388 125 96 54 27 BsrFI r'CCGG_y Cuts at: 0 920 3387 Size: 920 2467 BsrGI T'GTAC_A Cuts at: 0 264 1869 1937 3387 Size: 264 1605 68 1450 Fragments arranged by size: 1605 1450 264 68 BstAPI GCAn_nnn'nTGC Cuts at: 0 2659 3387 Size: 2659 728 BstXI CCAn_nnnn'nTGG Cuts at: 0 2835 3387 Size: 2835 552 BstYI r'GATC_y Cuts at: 0 4 71 480 702 1010 2058 2318 3072 Size: 4 67 409 222 308 1048 260 754 Cuts at: 3072 3387 Size: 315 Fragments arranged by size: 1048 754 409 315 308 260 222 67 4 BtgI C'CryG_G Cuts at: 0 1259 1269 2360 3387 Size: 1259 10 1091 1027 Fragments arranged by size: 1259 1091 1027 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 75 2165 3387 Size: 75 2090 1222 Fragments arranged by size: 2090 1222 75 BtsI GCAGTG_nn' Cuts at: 0 388 978 1628 1774 2254 3169 3387 Size: 388 590 650 146 480 915 218 Fragments arranged by size: 915 650 590 480 388 218 146 DraIII CAC_nnn'GTG Cuts at: 0 426 3387 Size: 426 2961 EaeI y'GGCC_r Cuts at: 0 1517 3318 3387 Size: 1517 1801 69 Fragments arranged by size: 1801 1517 69 EarI CTCTTCn'nnn_ Cuts at: 0 893 1241 2467 2598 3290 3387 Size: 893 348 1226 131 692 97 Fragments arranged by size: 1226 893 692 348 131 97 EcoICRI GAG'CTC Cuts at: 0 3255 3387 Size: 3255 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 196 199 391 934 2027 2211 2828 Size: 9 187 3 192 543 1093 184 617 Cuts at: 2828 3032 3076 3143 3387 Size: 204 44 67 244 Fragments arranged by size: 1093 617 543 244 204 192 187 184 67 44 9 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 2873 3387 Size: 2873 514 EcoO109I rG'GnC_Cy Cuts at: 0 1251 2543 2907 2961 3387 Size: 1251 1292 364 54 426 Fragments arranged by size: 1292 1251 426 364 54 EcoRV GAT'ATC Cuts at: 0 1163 1223 3387 Size: 1163 60 2164 Fragments arranged by size: 2164 1163 60 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2080 3369 3387 Size: 2080 1289 18 Fragments arranged by size: 2080 1289 18 HaeII r_GCGC'y Cuts at: 0 636 2196 3387 Size: 636 1560 1191 Fragments arranged by size: 1560 1191 636 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 58 90 100 132 1132 1147 1164 1179 Size: 58 32 10 32 1000 15 17 15 Cuts at: 1179 1357 1389 1828 1860 2611 2643 2710 2742 Size: 178 32 439 32 751 32 67 32 Cuts at: 2742 3089 3121 3387 Size: 347 32 266 Fragments arranged by size: 1000 751 439 347 266 178 67 58 32 32 32 32 32 32 32 17 15 15 10 HincII GTy'rAC Cuts at: 0 1860 1998 3387 Size: 1860 138 1389 Fragments arranged by size: 1860 1389 138 HindIII A'AGCT_T Cuts at: 0 1960 3387 Size: 1960 1427 HpaI GTT'AAC Cuts at: 0 1998 3387 Size: 1998 1389 KasI G'GCGC_C Cuts at: 0 632 3387 Size: 632 2755 KpnI G_GTAC'C Cuts at: 0 990 3387 Size: 990 2397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2428 2540 3155 3387 Size: 2428 112 615 232 Fragments arranged by size: 2428 615 232 112 MscI TGG'CCA Cuts at: 0 1519 3320 3387 Size: 1519 1801 67 Fragments arranged by size: 1801 1519 67 MspA1I CmG'CkG Cuts at: 0 772 1338 2289 2901 2982 3387 Size: 772 566 951 612 81 405 Fragments arranged by size: 951 772 612 566 405 81 NaeI GCC'GGC Cuts at: 0 922 3387 Size: 922 2465 NarI GG'CG_CC Cuts at: 0 633 3387 Size: 633 2754 NcoI C'CATG_G Cuts at: 0 1259 3387 Size: 1259 2128 NgoMIV G'CCGG_C Cuts at: 0 920 3387 Size: 920 2467 NheI G'CTAG_C Cuts at: 0 2994 3387 Size: 2994 393 NsiI A_TGCA'T Cuts at: 0 944 3387 Size: 944 2443 NspI r_CATG'y Cuts at: 0 942 1075 3387 Size: 942 133 2312 Fragments arranged by size: 2312 942 133 PasI CC'CwG_GG Cuts at: 0 1345 1905 3387 Size: 1345 560 1482 Fragments arranged by size: 1482 1345 560 PflMI CCAn_nnn'nTGG Cuts at: 0 2806 3387 Size: 2806 581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1023 1055 2631 2663 3387 Size: 1023 32 1576 32 724 Fragments arranged by size: 1576 1023 724 32 32 PpuMI rG'GwC_Cy Cuts at: 0 2907 2961 3387 Size: 2907 54 426 Fragments arranged by size: 2907 426 54 PsiI TTA'TAA Cuts at: 0 721 3387 Size: 721 2666 PspOMI G'GGCC_C Cuts at: 0 2542 3387 Size: 2542 845 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1413 1445 3387 Size: 1413 32 1942 Fragments arranged by size: 1942 1413 32 PstI C_TGCA'G Cuts at: 0 160 713 1836 3387 Size: 160 553 1123 1551 Fragments arranged by size: 1551 1123 553 160 PvuII CAG'CTG Cuts at: 0 772 2901 2982 3387 Size: 772 2129 81 405 Fragments arranged by size: 2129 772 405 81 SacI G_AGCT'C Cuts at: 0 3257 3387 Size: 3257 130 SfcI C'TryA_G Cuts at: 0 156 709 1832 1929 3387 Size: 156 553 1123 97 1458 Fragments arranged by size: 1458 1123 553 156 97 SfoI GGC'GCC Cuts at: 0 634 3387 Size: 634 2753 SmlI C'TyrA_G Cuts at: 0 484 1702 3387 Size: 484 1218 1685 Fragments arranged by size: 1685 1218 484 SphI G_CATG'C Cuts at: 0 942 3387 Size: 942 2445 StuI AGG'CCT Cuts at: 0 2478 3387 Size: 2478 909 StyI C'CwwG_G Cuts at: 0 339 437 1259 2275 2383 2566 2664 3026 Size: 339 98 822 1016 108 183 98 362 Cuts at: 3026 3387 Size: 361 Fragments arranged by size: 1016 822 362 361 339 183 108 98 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2214 2452 2931 3039 3080 3387 Size: 2214 238 479 108 41 307 Fragments arranged by size: 2214 479 307 238 108 41 TatI w'GTAC_w Cuts at: 0 264 788 1869 1937 3387 Size: 264 524 1081 68 1450 Fragments arranged by size: 1450 1081 524 264 68 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2352 2384 3292 3324 3387 Size: 2352 32 908 32 63 Fragments arranged by size: 2352 908 63 32 32 XbaI T'CTAG_A Cuts at: 0 189 3387 Size: 189 3198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 696 2077 2446 2898 2979 3387 Size: 696 1381 369 452 81 408 Fragments arranged by size: 1381 696 452 408 369 81 XmnI GAAnn'nnTTC Cuts at: 0 2759 3387 Size: 2759 628 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AloI AlwNI ApaI ApoI AvrII BaeI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PflMI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SacI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI BbvCI BcgI BclI BglI BsiEI BsiWI BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoRI FseI FspI FspAI MfeI MluI MslI NdeI NotI NruI PacI PciI PfoI PmeI PmlI PshAI PspXI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XhoI XmaI ZraI