################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbvCI BcgI BcnI BlnI BsaHI BseAI BsiWI BsmBI BsmFI BspDI BspEI BspMI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraII DraIII Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI MfeI MluI MroI MunI NarI NciI NcoI NdeI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 584 GTAGAC GT^[AC][GT]AC AflII 2976 CTTAAG C^TTAAG AlwNI 2095 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 2014 ATTAAT AT^TAAT AsnI 2014 ATTAAT AT^TAAT AvaI 3890 CTCGAG C^[CT]CG[GA]G BalI 326 TGGCCA TGG^CCA BanII 57 GGGCTC G[GA]GC[CT]^C BciVI 1897 GGATAC GTATCC(6/5) BclI 1551 TGATCA T^GATCA BfrI 2976 CTTAAG C^TTAAG BglI 124 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2824 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2824 GCTAAGC GC^T[ACGT]AGC BsaI 3640 GAGACC GGTCTC(1/5) BsmI 3573 GAATGC GAATGC(1/-1) BsoBI 3890 CTCGAG C^[CT]CG[GA]G BspLU11I 2521 ACATGT A^CATGT BsrBI 117 CCGCTC CCGCTC(-3/-3) BsrFI 124 GCCGGC [GA]^CCGG[CT] BstAPI 1225 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1850 CCAAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2824 GCTAAGC GC^T[ACGT]AGC Cfr10I 124 GCCGGC [GA]^CCGG[CT] DraI 1363 TTTAAA TTT^AAA DrdI 1016 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 62 CCTTGG C^C[AT][AT]GG FbaI 1551 TGATCA T^GATCA MluNI 326 TGGCCA TGG^CCA MscI 326 TGGCCA TGG^CCA NaeI 124 GCCGGC GCC^GGC NgoMIV 124 GCCGGC G^CCGGC PaeR7I 3890 CTCGAG C^TCGAG PshBI 2014 ATTAAT AT^TAAT PvuII 2432 CAGCTG CAG^CTG SpeI 1981 ACTAGT A^CTAGT SphI 1056 GCATGC GCATG^C StyI 62 CCTTGG C^C[AT][AT]GG XbaI 3319 TCTAGA T^CTAGA XcmI 53 CCACGGGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3890 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Asp700I 3304 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3719 GAAATAATTC BbrPI 212 CACGTG CAC^GTG 2587 CACGTG BglII 223 AGATCT A^GATCT 1970 AGATCT BsaAI 212 CACGTG [CT]AC^GT[GA] 2587 CACGTG BseRI 1092 CTCCTC GAGGAG(10/8) 3342 CTCCTC BsgI 28 CTGCAC GTGCAG(16/14) 1449 GTGCAG BsiEI 102 CGGCCG CG[GA][CT]^CG 122 CGGCCG BsmAI 3640 GAGAC GTCTC(1/5) 3794 GAGAC Bsp1407I 653 TGTACA T^GTACA 3781 TGTACA BspHI 1481 TCATGA T^CATGA 3987 TCATGA BsrGI 653 TGTACA T^GTACA 3781 TGTACA EagI 102 CGGCCG C^GGCCG 122 CGGCCG EclXI 102 CGGCCG C^GGCCG 122 CGGCCG Eco52I 102 CGGCCG C^GGCCG 122 CGGCCG FauI 145 CCCGC CCCGC(4/6) 166 GCGGG HgaI 1958 GACGC GACGC(5/10) 462 GCGTC HincII 2137 GTTGAC GT[CT]^[GA]AC 3037 GTTGAC HindII 2137 GTTGAC GT[CT]^[GA]AC 3037 GTTGAC KspI 41 CCGCGG CCGC^GG 146 CCGCGG NheI 1665 GCTAGC G^CTAGC 1878 GCTAGC PflMI 1835 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2401 CCAGGATTTGG PmaCI 212 CACGTG CAC^GTG 2587 CACGTG PmlI 212 CACGTG CAC^GTG 2587 CACGTG PstI 1993 CTGCAG CTGCA^G 2158 CTGCAG RcaI 1481 TCATGA T^CATGA 3987 TCATGA SacII 41 CCGCGG CCGC^GG 146 CCGCGG SapI 355 GAAGAGC GCTCTTC(1/4) 1873 GAAGAGC ScaI 2500 AGTACT AGT^ACT 3451 AGTACT SspBI 653 TGTACA T^GTACA 3781 TGTACA SspI 2382 AATATT AAT^ATT 2647 AATATT StuI 1556 AGGCCT AGG^CCT 1756 AGGCCT Van91I 1835 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2401 CCAGGATTTGG XmnI 3304 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3719 GAAATAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BanI BbsI BbvI BfaI BmyI BpmI BpuAI BsaBI BsaJI BsaWI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII