(Circular) (Six-base) MAPSORT of: pFN21AE5453.seq Check: 2034 from: 1 to: 5761 pFN21AE5453 5761 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 2024 3082 2024 Size: 1058 4703 AclI AA'CG_TT Cuts at: 1563 3060 3888 4261 1563 Size: 1497 828 373 3063 Fragments arranged by size: 3063 1497 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2205 2577 3824 5208 5476 1632 Size: 573 372 1247 1384 268 1917 Fragments arranged by size: 1917 1384 1247 573 372 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2592 4660 5637 1203 Size: 1389 2068 977 1327 Fragments arranged by size: 2068 1389 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2874 2906 2874 Size: 32 5729 AlwNI CAG_nnn'CTG Cuts at: 2740 3504 5076 2740 Size: 764 1572 3425 Fragments arranged by size: 3425 1572 764 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2623 3070 3296 3448 5557 1493 Size: 1130 447 226 152 2109 1697 Fragments arranged by size: 2109 1697 1130 447 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2394 3086 1870 Size: 73 152 299 692 4545 Fragments arranged by size: 4545 692 299 152 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2816 2849 2816 Size: 33 5728 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2024 3082 4537 618 Size: 325 217 627 237 1058 1455 1842 Fragments arranged by size: 1842 1455 1058 627 325 237 217 BanII G_rGCy'C Cuts at: 1391 3080 1391 Size: 1689 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BbvCI CC'TCA_GC Cuts at: 2259 2259 Size: 5761 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 2341 2634 1548 Size: 793 293 4675 Fragments arranged by size: 4675 793 293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 2696 3178 2696 Size: 482 5279 Bme1580I G_kGCm'C Cuts at: 1790 2401 3822 4978 1790 Size: 611 1421 1156 2573 Fragments arranged by size: 2573 1421 1156 611 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4421 1239 Size: 3182 2579 Bpu10I CC'TnA_GC Cuts at: 2259 2259 Size: 5761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3886 4751 5049 3886 Size: 865 298 4598 Fragments arranged by size: 4598 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2353 3552 493 Size: 711 1149 1199 2702 Fragments arranged by size: 2702 1199 1149 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3568 3950 4585 Size: 53 83 186 936 476 1559 382 635 Cuts at: 4585 275 Size: 1451 Fragments arranged by size: 1559 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2874 2891 2904 2921 1178 Size: 30 165 30 1471 17 13 17 4018 Fragments arranged by size: 4018 1471 165 30 30 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2892 2912 1186 Size: 1706 20 4035 Fragments arranged by size: 4035 1706 20 BseYI C'CCAG_C Cuts at: 4964 5460 4964 Size: 496 5265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 1163 Size: 573 405 4783 Fragments arranged by size: 4783 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3822 3907 4978 3822 Size: 85 1071 4605 Fragments arranged by size: 4605 1071 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2401 3080 3822 3907 4978 1391 Size: 399 611 679 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 611 399 85 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2890 3648 1394 Size: 429 1067 758 3507 Fragments arranged by size: 3507 1067 758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2958 4256 4430 65 Size: 1183 1710 1298 174 1396 Fragments arranged by size: 1710 1396 1298 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 2060 4405 4592 1700 Size: 110 217 33 2345 187 2869 Fragments arranged by size: 2869 2345 217 187 110 33 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2483 3091 3458 3843 3860 5312 5398 5410 Size: 511 608 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 608 511 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2895 2895 Size: 5761 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 3003 3277 4071 4091 3003 Size: 274 794 20 4673 Fragments arranged by size: 4673 794 274 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726 Size: 54 1425 50 275 18 1378 889 1629 Cuts at: 5726 8 Size: 43 Fragments arranged by size: 1629 1425 1378 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2050 2921 3032 3048 3691 1353 Size: 697 871 111 16 643 3423 Fragments arranged by size: 3423 871 697 643 111 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2577 3824 4421 5208 5476 1239 Size: 393 573 372 1247 597 787 268 1524 Fragments arranged by size: 1524 1247 787 597 573 393 372 268 EcoO109I rG'GnC_Cy Cuts at: 2902 2902 Size: 5761 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2219 2246 2219 Size: 27 5734 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2869 4908 1678 Size: 1191 2039 2531 Fragments arranged by size: 2531 2039 1191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2891 2923 4402 4434 4476 Size: 32 135 32 1319 32 1479 32 42 Cuts at: 4476 4508 1373 Size: 32 2626 Fragments arranged by size: 2626 1479 1319 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 2028 3086 2028 Size: 1058 4703 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3939 Fragments arranged by size: 3939 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 3719 4078 4237 518 Size: 648 1626 927 359 159 2042 Fragments arranged by size: 2042 1626 927 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2672 3177 3854 5002 5247 1606 Size: 1066 505 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 677 505 245 NaeI GCC'GGC Cuts at: 2062 2062 Size: 5761 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NgoMIV G'CCGG_C Cuts at: 2060 2060 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2432 2596 3119 4664 1132 Size: 97 1203 164 523 1545 2229 Fragments arranged by size: 2229 1545 1203 523 164 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 2592 4660 2592 Size: 2068 3693 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PfoI T'CCnGG_A Cuts at: 2403 2403 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PmlI CAC'GTG Cuts at: 2353 2353 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2874 2906 3830 3862 5343 5375 2874 Size: 32 924 32 1481 32 3260 Fragments arranged by size: 3260 1481 924 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2902 2902 Size: 5761 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 3113 830 Size: 2283 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2672 2672 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SapI GCTCTTCn'nnn_ Cuts at: 3032 3048 3032 Size: 16 5745 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2732 3109 4244 4925 5116 826 Size: 219 1687 377 1135 681 191 1471 Fragments arranged by size: 1687 1471 1135 681 377 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 2060 1700 Size: 360 5401 SmaI CCC'GGG Cuts at: 1872 2396 3088 1872 Size: 524 692 4545 Fragments arranged by size: 4545 692 524 SmlI C'TyrA_G Cuts at: 820 1017 1943 3865 4766 5028 820 Size: 197 926 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 2804 152 Size: 2652 3109 SphI G_CATG'C Cuts at: 1132 2432 3119 1132 Size: 1300 687 3774 Fragments arranged by size: 3774 1300 687 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 2479 3200 513 Size: 552 939 475 721 3074 Fragments arranged by size: 3074 939 721 552 475 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3788 3805 3958 4143 4588 325 Size: 1255 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2589 4007 5719 Size: 274 80 33 51 1443 612 1418 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1712 1443 1418 612 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 2394 3086 1870 Size: 524 692 4545 Fragments arranged by size: 4545 692 524 XmnI GAAnn'nnTTC Cuts at: 2190 3890 2190 Size: 1700 4061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI