(Circular) (Six-base) MAPSORT of: pFN21AE6175.seq Check: 9044 from: 1 to: 5038 pFN21AE6175 5038 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2848 278 Size: 53 83 186 936 1312 2468 Fragments arranged by size: 2468 1312 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2381 1937 Size: 444 4594 Acc65I G'GTAC_C Cuts at: 2359 2359 Size: 5038 AclI AA'CG_TT Cuts at: 1563 3165 3538 1563 Size: 1602 373 3063 Fragments arranged by size: 3063 1602 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3101 4485 4753 1632 Size: 1469 1384 268 1917 Fragments arranged by size: 1917 1469 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4841 AflIII A'CryG_T Cuts at: 1203 3937 4914 1203 Size: 2734 977 1327 Fragments arranged by size: 2734 1327 977 AgeI A'CCGG_T Cuts at: 3869 3869 Size: 5038 AhdI GACnn_n'nnGTC Cuts at: 3767 3767 Size: 5038 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5038 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5038 AlwNI CAG_nnn'CTG Cuts at: 2781 4353 2781 Size: 1572 3466 ApaLI G'TGCA_C Cuts at: 3095 4251 3095 Size: 1156 3882 ApoI r'AATT_y Cuts at: 1493 2266 2347 2573 2725 4834 1493 Size: 773 81 226 152 2109 1697 Fragments arranged by size: 2109 1697 773 226 152 81 AseI AT'TA_AT Cuts at: 160 1032 3592 160 Size: 872 2560 1606 Fragments arranged by size: 2560 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5038 AvaI C'yCGr_G Cuts at: 1870 1943 2363 1870 Size: 73 420 4545 Fragments arranged by size: 4545 420 73 BamHI G'GATC_C Cuts at: 2368 2735 2368 Size: 367 4671 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2359 3814 618 Size: 325 217 627 572 1455 1842 Fragments arranged by size: 1842 1455 627 572 325 217 BanII G_rGCy'C Cuts at: 1391 2357 1391 Size: 966 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2158 928 Size: 971 259 3808 Fragments arranged by size: 3808 971 259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3229 3263 1903 Size: 34 1292 34 3678 Fragments arranged by size: 3678 1292 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2930 4140 2930 Size: 1210 3828 BclI T'GATC_A Cuts at: 1548 1548 Size: 5038 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3649 136 Size: 107 122 71 631 605 1977 1525 Fragments arranged by size: 1977 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2226 5033 2226 Size: 2807 2231 BlpI GC'TnA_GC Cuts at: 2455 2455 Size: 5038 Bme1580I G_kGCm'C Cuts at: 1790 2203 3099 4255 1790 Size: 413 896 1156 2573 Fragments arranged by size: 2573 1156 896 413 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5038 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2102 2769 3727 Size: 416 53 83 399 24 685 667 958 Cuts at: 3727 442 Size: 1753 Fragments arranged by size: 1753 958 685 667 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5038 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3698 1239 Size: 2459 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3163 4028 4326 3163 Size: 865 298 3875 Fragments arranged by size: 3875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3701 882 Size: 617 2202 2219 Fragments arranged by size: 2219 2202 617 BsaAI yAC'GTr Cuts at: 493 1204 2829 493 Size: 711 1625 2702 Fragments arranged by size: 2702 1625 711 BsaBI GATnn'nnATC Cuts at: 5032 5032 Size: 5038 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2845 3227 3862 275 Size: 53 83 186 936 1312 382 635 1451 Fragments arranged by size: 1451 1312 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3471 3869 4143 4290 4701 3471 Size: 398 274 147 411 3808 Fragments arranged by size: 3808 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4813 Fragments arranged by size: 4813 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5038 BseYI C'CCAG_C Cuts at: 4241 4737 4241 Size: 496 4542 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2488 3249 4277 1540 Size: 275 186 487 761 1028 2301 Fragments arranged by size: 2301 1028 761 487 275 186 BsiHKAI G_wGCw'C Cuts at: 3099 3184 4255 3099 Size: 85 1071 3882 Fragments arranged by size: 3882 1071 85 BsmI GAATG_Cn' Cuts at: 2549 2642 2549 Size: 93 4945 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2203 2357 3099 3184 4255 1391 Size: 399 413 154 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 413 399 154 85 BspEI T'CCGG_A Cuts at: 4701 4701 Size: 5038 BspHI T'CATG_A Cuts at: 2927 4657 2927 Size: 1730 3308 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2393 844 Size: 1549 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2925 1394 Size: 429 1102 3507 Fragments arranged by size: 3507 1102 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3533 3707 65 Size: 1183 2285 174 1396 Fragments arranged by size: 2285 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3682 3869 1700 Size: 110 1872 187 2869 Fragments arranged by size: 2869 1872 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5038 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5038 BssSI C'ACGA_G Cuts at: 3098 4110 3098 Size: 1012 4026 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5038 BstBI TT'CG_AA Cuts at: 2056 3842 2056 Size: 1786 3252 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5038 BstYI r'GATC_y Cuts at: 1972 2226 2368 2735 3120 3137 4589 4675 4687 Size: 254 142 367 385 17 1452 86 12 Cuts at: 4687 5033 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 254 142 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3547 Fragments arranged by size: 3547 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4299 Fragments arranged by size: 4299 683 56 BtsI GCAGTG_nn' Cuts at: 2249 2554 3348 3368 2249 Size: 305 794 20 3919 Fragments arranged by size: 3919 794 305 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5038 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5003 DraI TTT'AAA Cuts at: 2342 2342 Size: 5038 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4045 809 Size: 507 2729 1802 Fragments arranged by size: 2729 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2485 3374 5003 Size: 54 1425 50 275 18 655 889 1629 Cuts at: 5003 8 Size: 43 Fragments arranged by size: 1629 1425 889 655 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2485 1537 Size: 275 673 4090 Fragments arranged by size: 4090 673 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 2968 1353 Size: 900 715 3423 Fragments arranged by size: 3423 900 715 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3625 3999 4145 387 Size: 1064 138 2036 374 146 1280 Fragments arranged by size: 2036 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3101 3698 4485 4753 1239 Size: 393 1469 597 787 268 1524 Fragments arranged by size: 1524 1469 787 597 393 268 EcoRI G'AATT_C Cuts at: 2347 2725 2347 Size: 378 4660 FspI TGC'GCA Cuts at: 3544 3544 Size: 5038 HaeII r_GCGC'y Cuts at: 1678 4185 1678 Size: 2507 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3679 3711 3753 3785 1373 Size: 32 135 32 2107 32 42 32 2626 Fragments arranged by size: 2626 2107 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2382 2628 669 Size: 988 281 444 246 3079 Fragments arranged by size: 3079 988 444 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5038 HpaI GTT'AAC Cuts at: 2628 2628 Size: 5038 KpnI G_GTAC'C Cuts at: 2363 2363 Size: 5038 MfeI C'AATT_G Cuts at: 2637 2637 Size: 5038 MluI A'CGCG_T Cuts at: 4914 4914 Size: 5038 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4152 4336 1305 Size: 2847 184 2007 Fragments arranged by size: 2847 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3216 Fragments arranged by size: 3216 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2996 3355 3514 518 Size: 648 1830 359 159 2042 Fragments arranged by size: 2042 1830 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2454 3131 4279 4524 1606 Size: 848 677 1148 245 2120 Fragments arranged by size: 2120 1148 848 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3547 Fragments arranged by size: 3547 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5038 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5038 NotI GC'GGCC_GC Cuts at: 2485 2485 Size: 5038 NspI r_CATG'y Cuts at: 1132 1229 2396 3941 1132 Size: 97 1167 1545 2229 Fragments arranged by size: 2229 1545 1167 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5038 PciI A'CATG_T Cuts at: 3937 3937 Size: 5038 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5038 PmeI GTTT'AAAC Cuts at: 2342 2342 Size: 5038 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3107 3139 4620 4652 3107 Size: 32 1481 32 3493 Fragments arranged by size: 3493 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5038 PsiI TTA'TAA Cuts at: 2172 2608 2172 Size: 436 4602 PstI C_TGCA'G Cuts at: 830 2080 2390 830 Size: 1250 310 3478 Fragments arranged by size: 3478 1250 310 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5038 SalI G'TCGA_C Cuts at: 1936 2380 1936 Size: 444 4594 SbfI CC_TGCA'GG Cuts at: 2390 2390 Size: 5038 ScaI AGT'ACT Cuts at: 3286 4998 3286 Size: 1712 3326 SfcI C'TryA_G Cuts at: 826 1045 2076 2386 3521 4202 4393 826 Size: 219 1031 310 1135 681 191 1471 Fragments arranged by size: 1471 1135 1031 681 310 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5038 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5038 SmaI CCC'GGG Cuts at: 1872 2365 1872 Size: 493 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3142 4043 4305 820 Size: 197 926 1199 901 262 1553 Fragments arranged by size: 1553 1199 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2829 493 Size: 2336 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5038 SphI G_CATG'C Cuts at: 1132 2396 1132 Size: 1264 3774 SspI AAT'ATT Cuts at: 5 52 2962 5 Size: 47 2910 2081 Fragments arranged by size: 2910 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5038 StyI C'CwwG_G Cuts at: 513 1065 2004 2477 513 Size: 552 939 473 3074 Fragments arranged by size: 3074 939 552 473 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3065 3082 3235 3420 3865 325 Size: 1255 1485 17 153 185 445 1498 Fragments arranged by size: 1498 1485 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3284 4996 96 Size: 274 80 33 51 1443 1307 1712 138 Fragments arranged by size: 1712 1443 1307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4998 5030 4998 Size: 32 5006 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5038 XbaI T'CTAG_A Cuts at: 2374 2374 Size: 5038 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5038 XmaI C'CCGG_G Cuts at: 1870 2363 1870 Size: 493 4545 XmnI GAAnn'nnTTC Cuts at: 3167 3167 Size: 5038 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2846 276 Size: 53 83 186 936 1312 2468 Fragments arranged by size: 2468 1312 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI