################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccII AccIII AflII AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AviII AvrII BanI BbeI BbvCI BfrI BglI BlnI BlpI Bpu1102I BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstUI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I FauI FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AclI 1312 AACGTT AA^CGTT AcyI 1493 GACGCC G[GA]^CG[CT]C AlwNI 1568 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 997 ATTAAT AT^TAAT AsnI 997 ATTAAT AT^TAAT Asp700I 663 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 575 CCCGAG C^[CT]CG[GA]G BamHI 1773 GGATCC G^GATCC BanII 1941 GAGCTC G[GA]GC[CT]^C BbrPI 1840 CACGTG CAC^GTG BciVI 2342 GGATAC GTATCC(6/5) BcnI 799 CCCGG CC^[GC]GG BsaHI 1493 GACGCC G[GA]^CG[CT]C BsaWI 1528 ACCGGA [AT]^CCGG[AT] BsgI 1088 CTGCAC GTGCAG(16/14) BsoBI 575 CCCGAG C^[CT]CG[GA]G BspLU11I 1015 ACATGT A^CATGT BspMI 2326 GCAGGT ACCTGC(4/8) BsrDI 1757 GCAATG GCAATG(2/0) Bst1107I 2369 GTATAC GTA^TAC BstEII 2329 GGTCACC G^GT[ACGT]ACC BstPI 2329 GGTCACC G^GT[ACGT]ACC BstZ17I 2369 GTATAC GTA^TAC DsaI 1166 CCATGG C^C[GA][CT]GG EarI 1177 CTCTTC CTCTTC(1/4) EcoNI 248 CCTTGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2329 GGTCACC G^GT[ACGT]ACC EcoRI 663 GAATTC G^AATTC EcoT22I 911 ATGCAT ATGCA^T Hin1I 1493 GACGCC G[GA]^CG[CT]C HindIII 2130 AAGCTT A^AGCTT Ksp632I 1177 CTCTTC CTCTTC(1/4) MfeI 854 CAATTG C^AATTG MunI 854 CAATTG C^AATTG NciI 799 CCCGG CC^[GC]GG NcoI 1166 CCATGG C^CATGG NdeI 914 CATATG CA^TATG NheI 1998 GCTAGC G^CTAGC NsiI 911 ATGCAT ATGCA^T NspI 1015 ACATGT [GA]CATG^[CT] PflMI 1438 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1840 CACGTG CAC^GTG PmlI 1840 CACGTG CAC^GTG PshBI 997 ATTAAT AT^TAAT Psp1406I 1312 AACGTT AA^CGTT PstI 2292 CTGCAG CTGCA^G PvuII 1571 CAGCTG CAG^CTG SacI 1941 GAGCTC GAGCT^C TaqI 366 TCGA T^CGA TthHB8I 366 TCGA T^CGA Van91I 1438 CCATTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 663 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 2184 GTCTAC GT^[AC][GT]AC 2369 GTATAC AflIII 1015 ACATGT A^C[GA][CT]GT 1704 ACACGT Alw44I 803 GTGCAC G^TGCAC 2042 GTGCAC ApaLI 803 GTGCAC G^TGCAC 2042 GTGCAC BcgI 408 GCAGAACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2085 GCACTTATTTCG BfaI 1615 CTAG C^TAG 1999 CTAG BglII 265 AGATCT A^GATCT 1853 AGATCT BpmI 280 CTCCAG CTGGAG(16/14) 379 CTCCAG BseRI 1244 CTCCTC GAGGAG(10/8) 2206 GAGGAG DraII 1078 GGGACCC [GA]G^G[ACGT]CC[CT] 2097 GGGTCCC EcoO109I 1078 GGGACCC [GA]G^G[ACGT]CC[CT] 2097 GGGTCCC EcoRV 691 GATATC GAT^ATC 1321 GATATC HgaI 298 GACGC GACGC(5/10) 1493 GACGC HincII 820 GTTGAC GT[CT]^[GA]AC 1504 GTTGAC HindII 820 GTTGAC GT[CT]^[GA]AC 1504 GTTGAC MaeI 1615 CTAG C^TAG 1999 CTAG MspA1I 967 CCGCTG C[AC]G^C[GT]G 1571 CAGCTG PpuMI 1078 GGGACCC [GA]G^G[AT]CC[CT] 2097 GGGTCCC SmlI 1938 CTTGAG C^T[CT][GA]AG 2465 CTCAAG SspI 709 AATATT AAT^ATT 809 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BalI BbsI BbvI BclI BmyI BpuAI BsaAI BsaBI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII