(Circular) (Six-base) MAPSORT of: pFN21AE2024.seq Check: 6054 from: 1 to: 6517 pFN21AE2024 6517 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3703 3703 Size: 6517 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4327 278 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3860 1937 Size: 1923 4594 Acc65I G'GTAC_C Cuts at: 3838 3838 Size: 6517 AclI AA'CG_TT Cuts at: 1563 2228 4644 5017 1563 Size: 665 2416 373 3063 Fragments arranged by size: 3063 2416 665 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2278 4580 5964 6232 1632 Size: 646 2302 1384 268 1917 Fragments arranged by size: 2302 1917 1384 646 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6320 AflIII A'CryG_T Cuts at: 1203 3008 5416 6393 1203 Size: 1805 2408 977 1327 Fragments arranged by size: 2408 1805 1327 977 AgeI A'CCGG_T Cuts at: 5348 5348 Size: 6517 AhdI GACnn_n'nnGTC Cuts at: 2543 5246 2543 Size: 2703 3814 AleI CACnn'nnGTG Cuts at: 1166 3654 1166 Size: 2488 4029 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6517 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2430 2462 2430 Size: 32 6485 AlwNI CAG_nnn'CTG Cuts at: 3588 3667 4260 5832 3588 Size: 79 593 1572 4273 Fragments arranged by size: 4273 1572 593 79 ApaLI G'TGCA_C Cuts at: 4574 5730 4574 Size: 1156 5361 ApoI r'AATT_y Cuts at: 1493 2495 3296 3826 4052 4204 6313 1493 Size: 1002 801 530 226 152 2109 1697 Fragments arranged by size: 2109 1697 1002 801 530 226 152 AseI AT'TA_AT Cuts at: 160 1032 5071 160 Size: 872 4039 1606 Fragments arranged by size: 4039 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 AvaI C'yCGr_G Cuts at: 1870 1943 2429 3781 3842 1870 Size: 73 486 1352 61 4545 Fragments arranged by size: 4545 1352 486 73 61 BamHI G'GATC_C Cuts at: 3180 3847 4214 3180 Size: 667 367 5483 Fragments arranged by size: 5483 667 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2270 2360 3838 5293 618 Size: 325 217 627 483 90 1478 1455 1842 Fragments arranged by size: 1842 1478 1455 627 483 325 217 90 BanII G_rGCy'C Cuts at: 1391 2200 2592 3740 3836 1391 Size: 809 392 1148 96 4072 Fragments arranged by size: 4072 1148 809 392 96 BbeI G_GCGC'C Cuts at: 2274 2274 Size: 6517 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2147 928 Size: 971 248 5298 Fragments arranged by size: 5298 971 248 BbvCI CC'TCA_GC Cuts at: 2895 3351 2895 Size: 456 6061 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4708 4742 1903 Size: 34 2771 34 3678 Fragments arranged by size: 3678 2771 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4409 5619 3314 Size: 1095 1210 4212 Fragments arranged by size: 4212 1210 1095 BclI T'GATC_A Cuts at: 1548 3028 3438 1548 Size: 1480 410 4627 Fragments arranged by size: 4627 1480 410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5128 136 Size: 107 122 71 631 605 3456 1525 Fragments arranged by size: 3456 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3336 6512 3336 Size: 3176 3341 BlpI GC'TnA_GC Cuts at: 3934 3934 Size: 6517 Bme1580I G_kGCm'C Cuts at: 1790 4578 5734 1790 Size: 2788 1156 2573 Fragments arranged by size: 2788 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3011 1318 Size: 1693 4824 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 3266 3643 Size: 416 53 83 399 24 1206 643 377 Cuts at: 3643 4248 5206 442 Size: 605 958 1753 Fragments arranged by size: 1753 1206 958 643 605 416 399 377 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6517 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2125 2268 2751 2125 Size: 143 483 5891 Fragments arranged by size: 5891 483 143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2721 5177 1239 Size: 1482 2456 2579 Fragments arranged by size: 2579 2456 1482 Bpu10I CC'TnA_GC Cuts at: 2895 3351 2895 Size: 456 6061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2175 4642 5507 5805 2175 Size: 2467 865 298 2887 Fragments arranged by size: 2887 2467 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2530 5180 882 Size: 617 1031 2650 2219 Fragments arranged by size: 2650 2219 1031 617 BsaAI yAC'GTr Cuts at: 493 1204 3325 4308 493 Size: 711 2121 983 2702 Fragments arranged by size: 2702 2121 983 711 BsaBI GATnn'nnATC Cuts at: 6511 6511 Size: 6517 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2271 3198 4324 4706 Size: 53 83 186 936 738 927 1126 382 Cuts at: 4706 5341 275 Size: 635 1451 Fragments arranged by size: 1451 1126 936 927 738 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4950 5348 5622 5769 6180 4950 Size: 398 274 147 411 5287 Fragments arranged by size: 5287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2430 2460 2872 2902 2927 Size: 30 165 30 1027 30 412 30 25 Cuts at: 2927 2957 1178 Size: 30 4738 Fragments arranged by size: 4738 1027 412 165 30 30 30 30 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2151 2311 2314 2582 2680 3347 3368 3697 Size: 965 160 3 268 98 667 21 329 Cuts at: 3697 1186 Size: 4006 Fragments arranged by size: 4006 965 667 329 268 160 98 21 3 BseYI C'CCAG_C Cuts at: 2350 2640 2957 3675 3731 5720 6216 2350 Size: 290 317 718 56 1989 496 2651 Fragments arranged by size: 2651 1989 718 496 317 290 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5944 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3474 3967 4728 5756 1540 Size: 275 186 1473 493 761 1028 2301 Fragments arranged by size: 2301 1473 1028 761 493 275 186 BsiHKAI G_wGCw'C Cuts at: 2038 2200 2592 4578 4663 5734 2038 Size: 162 392 1986 85 1071 2821 Fragments arranged by size: 2821 1986 1071 392 162 85 BsmI GAATG_Cn' Cuts at: 4028 4121 4028 Size: 93 6424 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2200 2592 3740 3836 4578 4663 Size: 399 248 162 392 1148 96 742 85 Cuts at: 4663 5734 1391 Size: 1071 2174 Fragments arranged by size: 2174 1148 1071 742 399 392 248 162 96 85 BspEI T'CCGG_A Cuts at: 6180 6180 Size: 6517 BspHI T'CATG_A Cuts at: 4406 6136 4406 Size: 1730 4787 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3587 3703 3872 844 Size: 2743 116 169 3489 Fragments arranged by size: 3489 2743 169 116 BsrBI CCG'CTC Cuts at: 1394 1823 3421 4404 1394 Size: 429 1598 983 3507 Fragments arranged by size: 3507 1598 983 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2305 2422 2922 5012 5186 65 Size: 1183 1057 117 500 2090 174 1396 Fragments arranged by size: 2090 1396 1183 1057 500 174 117 BsrFI r'CCGG_y Cuts at: 1700 1810 5161 5348 1700 Size: 110 3351 187 2869 Fragments arranged by size: 3351 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6517 BssSI C'ACGA_G Cuts at: 2242 4577 5589 2242 Size: 2335 1012 3170 Fragments arranged by size: 3170 2335 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6517 BstBI TT'CG_AA Cuts at: 5321 5321 Size: 6517 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6517 BstXI CCAn_nnnn'nTGG Cuts at: 2864 2864 Size: 6517 BstYI r'GATC_y Cuts at: 1972 3180 3336 3847 4214 4599 4616 6068 6154 Size: 1208 156 511 367 385 17 1452 86 Cuts at: 6154 6166 6512 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1208 511 385 367 346 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2972 2972 Size: 6517 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5965 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3398 487 Size: 683 56 2172 3606 Fragments arranged by size: 3606 2172 683 56 BtsI GCAGTG_nn' Cuts at: 2596 3589 4033 4827 4847 2596 Size: 993 444 794 20 4266 Fragments arranged by size: 4266 993 794 444 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6517 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6482 DraI TTT'AAA Cuts at: 3821 3821 Size: 6517 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5524 809 Size: 507 4208 1802 Fragments arranged by size: 4208 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2610 2919 3559 Size: 54 1425 50 275 18 780 309 640 Cuts at: 3559 3964 4853 6482 8 Size: 405 889 1629 43 Fragments arranged by size: 1629 1425 889 780 640 405 309 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3964 1537 Size: 275 2152 4090 Fragments arranged by size: 4090 2152 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2317 2720 3054 4447 1353 Size: 964 403 334 1393 3423 Fragments arranged by size: 3423 1393 964 403 334 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3431 3512 5104 5478 5624 387 Size: 1064 138 1842 81 1592 374 146 1280 Fragments arranged by size: 1842 1592 1280 1064 374 146 138 81 EcoICRI GAG'CTC Cuts at: 2198 2590 2198 Size: 392 6125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2278 2721 4580 5177 5964 6232 1239 Size: 393 646 443 1859 597 787 268 1524 Fragments arranged by size: 1859 1524 787 646 597 443 393 268 EcoO109I rG'GnC_Cy Cuts at: 2968 2968 Size: 6517 EcoRI G'AATT_C Cuts at: 3296 3826 4204 3296 Size: 530 378 5609 Fragments arranged by size: 5609 530 378 EcoRV GAT'ATC Cuts at: 2010 2010 Size: 6517 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2043 2584 2043 Size: 541 5976 FspI TGC'GCA Cuts at: 2602 5023 2602 Size: 2421 4096 HaeII r_GCGC'y Cuts at: 1678 2274 3379 5664 1678 Size: 596 1105 2285 2531 Fragments arranged by size: 2531 2285 1105 596 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2529 2561 3796 3828 5158 Size: 32 135 32 957 32 1235 32 1330 Cuts at: 5158 5190 5232 5264 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1330 1235 957 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2232 2686 3262 3774 3861 4107 Size: 988 281 294 454 576 512 87 246 Cuts at: 4107 669 Size: 3079 Fragments arranged by size: 3079 988 576 512 454 294 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 6517 HpaI GTT'AAC Cuts at: 2232 4107 2232 Size: 1875 4642 KasI G'GCGC_C Cuts at: 2270 2270 Size: 6517 KpnI G_GTAC'C Cuts at: 3842 3842 Size: 6517 MfeI C'AATT_G Cuts at: 2487 4116 2487 Size: 1629 4888 MluI A'CGCG_T Cuts at: 6393 6393 Size: 6517 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2189 5631 5815 1305 Size: 884 3442 184 2007 Fragments arranged by size: 3442 2007 884 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 2921 10 Size: 54 1425 343 780 309 3606 Fragments arranged by size: 3606 1425 780 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 3654 4475 4834 4993 518 Size: 648 2488 821 359 159 2042 Fragments arranged by size: 2488 2042 821 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 2653 2827 3726 3933 4610 5758 6003 Size: 738 309 174 899 207 677 1148 245 Cuts at: 6003 1606 Size: 2120 Fragments arranged by size: 2120 1148 899 738 677 309 245 207 174 NarI GG'CG_CC Cuts at: 2271 2271 Size: 6517 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5965 NdeI CA'TA_TG Cuts at: 387 387 Size: 6517 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6517 NotI GC'GGCC_GC Cuts at: 3964 3964 Size: 6517 NspI r_CATG'y Cuts at: 1132 1229 3875 5420 1132 Size: 97 2646 1545 2229 Fragments arranged by size: 2646 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6517 PciI A'CATG_T Cuts at: 5416 5416 Size: 6517 PflMI CCAn_nnn'nTGG Cuts at: 1108 2288 2357 1108 Size: 1180 69 5268 Fragments arranged by size: 5268 1180 69 PmeI GTTT'AAAC Cuts at: 3821 3821 Size: 6517 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2430 2462 4586 4618 6099 6131 2430 Size: 32 2124 32 1481 32 2816 Fragments arranged by size: 2816 2124 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2968 2968 Size: 6517 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6517 PsiI TTA'TAA Cuts at: 4087 4087 Size: 6517 PstI C_TGCA'G Cuts at: 830 2406 2583 3399 3584 3672 3700 3869 830 Size: 1576 177 816 185 88 28 169 3478 Fragments arranged by size: 3478 1576 816 185 177 169 88 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6517 PvuII CAG'CTG Cuts at: 2344 2653 2344 Size: 309 6208 SacI G_AGCT'C Cuts at: 2200 2592 2200 Size: 392 6125 SalI G'TCGA_C Cuts at: 1936 3859 1936 Size: 1923 4594 SbfI CC_TGCA'GG Cuts at: 3672 3869 3672 Size: 197 6320 ScaI AGT'ACT Cuts at: 4765 6477 4765 Size: 1712 4805 SfcI C'TryA_G Cuts at: 826 1045 2256 2402 2579 2935 3395 3580 3668 Size: 219 1211 146 177 356 460 185 88 Cuts at: 3668 3696 3865 5000 5681 5872 826 Size: 28 169 1135 681 191 1471 Fragments arranged by size: 1471 1211 1135 681 460 356 219 191 185 177 169 146 88 28 SfoI GGC'GCC Cuts at: 2272 2272 Size: 6517 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6517 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6517 SmaI CCC'GGG Cuts at: 1872 3783 3844 1872 Size: 1911 61 4545 Fragments arranged by size: 4545 1911 61 SmlI C'TyrA_G Cuts at: 820 1017 1943 2190 4621 5522 5784 820 Size: 197 926 247 2431 901 262 1553 Fragments arranged by size: 2431 1553 926 901 262 247 197 SnaBI TAC'GTA Cuts at: 493 4308 493 Size: 3815 2702 SpeI A'CTAG_T Cuts at: 152 2467 152 Size: 2315 4202 SphI G_CATG'C Cuts at: 1132 3875 1132 Size: 2743 3774 SspI AAT'ATT Cuts at: 5 52 2084 4441 5 Size: 47 2032 2357 2081 Fragments arranged by size: 2357 2081 2032 47 StuI AGG'CCT Cuts at: 1517 3310 1517 Size: 1793 4724 StyI C'CwwG_G Cuts at: 513 1065 3956 513 Size: 552 2891 3074 Fragments arranged by size: 3074 2891 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2299 4544 4561 4714 4899 5344 325 Size: 1255 719 2245 17 153 185 445 1498 Fragments arranged by size: 2245 1498 1255 719 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4763 6475 96 Size: 274 80 33 51 1443 2786 1712 138 Fragments arranged by size: 2786 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6477 6509 6477 Size: 32 6485 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6517 XbaI T'CTAG_A Cuts at: 3853 3853 Size: 6517 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3336 3653 3336 Size: 317 6200 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6517 XmaI C'CCGG_G Cuts at: 1870 3781 3842 1870 Size: 1911 61 4545 Fragments arranged by size: 4545 1911 61 XmnI GAAnn'nnTTC Cuts at: 2209 2387 2445 2496 3300 3790 4646 2209 Size: 178 58 51 804 490 856 4080 Fragments arranged by size: 4080 856 804 490 178 58 51 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4325 276 Size: 53 83 186 936 2791 2468 Fragments arranged by size: 2791 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI