(Circular) (Six-base) MAPSORT of: pFN21AE3130.seq Check: 136 from: 1 to: 5674 pFN21AE3130 5674 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2714 3017 1937 Size: 777 303 4594 Fragments arranged by size: 4594 777 303 Acc65I G'GTAC_C Cuts at: 2082 2995 2082 Size: 913 4761 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 3737 5121 5389 1632 Size: 843 1262 1384 268 1917 Fragments arranged by size: 1917 1384 1262 843 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 4573 5550 1203 Size: 3370 977 1327 Fragments arranged by size: 3370 1327 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 2140 3417 4989 2140 Size: 1277 1572 2825 Fragments arranged by size: 2825 1572 1277 ApaLI G'TGCA_C Cuts at: 3731 4887 3731 Size: 1156 4518 ApoI r'AATT_y Cuts at: 1493 2181 2726 2983 3209 3361 5470 1493 Size: 688 545 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 688 545 257 226 152 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2999 1870 Size: 73 70 986 4545 Fragments arranged by size: 4545 986 73 70 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 2082 2995 4450 618 Size: 325 217 627 251 44 913 1455 1842 Fragments arranged by size: 1842 1455 913 627 325 251 217 44 BanII G_rGCy'C Cuts at: 1391 2013 2794 2993 1391 Size: 622 781 199 4072 Fragments arranged by size: 4072 781 622 199 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2676 928 Size: 971 777 3926 Fragments arranged by size: 3926 971 777 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3865 3899 1903 Size: 34 1928 34 3678 Fragments arranged by size: 3678 1928 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BfrBI ATG'CAT Cuts at: 2906 2906 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4285 136 Size: 107 122 71 631 605 2613 1525 Fragments arranged by size: 2613 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 3091 3091 Size: 5674 Bme1580I G_kGCm'C Cuts at: 1790 3735 4891 1790 Size: 1945 1156 2573 Fragments arranged by size: 2573 1945 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3405 4363 442 Size: 416 53 83 399 24 1988 958 1753 Fragments arranged by size: 1988 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2703 4334 1239 Size: 1076 388 1631 2579 Fragments arranged by size: 2579 1631 1076 388 Bpu10I CC'TnA_GC Cuts at: 2914 2914 Size: 5674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 2650 2801 3799 4664 4962 2387 Size: 263 151 998 865 298 3099 Fragments arranged by size: 3099 998 865 298 263 151 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4337 882 Size: 617 2838 2219 Fragments arranged by size: 2838 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 5668 5668 Size: 5674 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3481 3863 4498 275 Size: 53 83 186 936 1948 382 635 1451 Fragments arranged by size: 1948 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5449 Fragments arranged by size: 5449 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2239 2832 1186 Size: 1053 593 4028 Fragments arranged by size: 4028 1053 593 BseYI C'CCAG_C Cuts at: 2077 4877 5373 2077 Size: 2800 496 2378 Fragments arranged by size: 2800 2378 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2074 1163 Size: 573 338 4763 Fragments arranged by size: 4763 573 338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3124 3885 4913 1540 Size: 275 186 1123 761 1028 2301 Fragments arranged by size: 2301 1123 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2614 2794 3735 3820 4891 2614 Size: 180 941 85 1071 3397 Fragments arranged by size: 3397 1071 941 180 85 BsmI GAATG_Cn' Cuts at: 2203 3185 3278 2203 Size: 982 93 4599 Fragments arranged by size: 4599 982 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2398 2398 Size: 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2614 2794 2993 3735 3820 4891 Size: 399 223 601 180 199 742 85 1071 Cuts at: 4891 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 601 399 223 199 180 85 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 3029 844 Size: 1202 983 3489 Fragments arranged by size: 3489 1202 983 BsrBI CCG'CTC Cuts at: 1394 1823 3561 1394 Size: 429 1738 3507 Fragments arranged by size: 3507 1738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4169 4343 65 Size: 1183 2921 174 1396 Fragments arranged by size: 2921 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2049 4318 4505 1700 Size: 110 239 2269 187 2869 Fragments arranged by size: 2869 2269 239 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5674 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5674 BstBI TT'CG_AA Cuts at: 4478 4478 Size: 5674 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstXI CCAn_nnnn'nTGG Cuts at: 2491 2491 Size: 5674 BstYI r'GATC_y Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669 Size: 1032 367 385 17 1452 86 12 346 Cuts at: 5669 1972 Size: 1977 Fragments arranged by size: 1977 1452 1032 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2715 2715 Size: 5674 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4183 Fragments arranged by size: 4183 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2135 487 Size: 683 56 909 4026 Fragments arranged by size: 4026 909 683 56 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2560 3121 Size: 54 1425 50 275 18 420 310 561 Cuts at: 3121 4010 5639 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 561 420 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3121 1537 Size: 275 1309 4090 Fragments arranged by size: 4090 1309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2124 2216 3604 1353 Size: 756 15 92 1388 3423 Fragments arranged by size: 3423 1388 756 92 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2792 2792 Size: 5674 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2475 2703 3737 4334 5121 5389 Size: 393 683 160 228 1034 597 787 268 Cuts at: 5389 1239 Size: 1524 Fragments arranged by size: 1524 1034 787 683 597 393 268 228 160 EcoNI CCTnn'n_nnAGG Cuts at: 2700 2700 Size: 5674 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 EcoRV GAT'ATC Cuts at: 2388 2388 Size: 5674 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 4821 1678 Size: 3143 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2134 2166 4315 4347 4389 Size: 32 135 32 562 32 2149 32 42 Cuts at: 4389 4421 1373 Size: 32 2626 Fragments arranged by size: 2626 2149 562 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2347 3018 3264 669 Size: 988 281 409 671 246 3079 Fragments arranged by size: 3079 988 671 409 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5674 HpaI GTT'AAC Cuts at: 2347 3264 2347 Size: 917 4757 KpnI G_GTAC'C Cuts at: 2086 2999 2086 Size: 913 4761 MfeI C'AATT_G Cuts at: 2300 3273 2300 Size: 973 4701 MluI A'CGCG_T Cuts at: 5550 5550 Size: 5674 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2238 4788 4972 1305 Size: 933 2550 184 2007 Fragments arranged by size: 2550 2007 933 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2562 10 Size: 54 1425 343 420 310 3122 Fragments arranged by size: 3122 1425 420 343 310 54 MslI CAynn'nnrTG Cuts at: 518 1166 2907 3632 3991 4150 518 Size: 648 1741 725 359 159 2042 Fragments arranged by size: 2042 1741 725 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2645 2762 3090 3767 4915 5160 1606 Size: 531 508 117 328 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 531 508 328 245 117 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4183 Fragments arranged by size: 4183 939 552 NdeI CA'TA_TG Cuts at: 387 2490 2908 387 Size: 2103 418 3153 Fragments arranged by size: 3153 2103 418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NsiI A_TGCA'T Cuts at: 2908 2908 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 2906 3032 4577 1132 Size: 97 1677 126 1545 2229 Fragments arranged by size: 2229 1677 1545 126 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5674 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 2558 1108 Size: 1450 4224 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 2928 3244 2928 Size: 316 5358 PstI C_TGCA'G Cuts at: 830 3026 830 Size: 2196 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5674 PvuII CAG'CTG Cuts at: 2137 2762 2137 Size: 625 5049 SacI G_AGCT'C Cuts at: 2794 2794 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SapI GCTCTTCn'nnn_ Cuts at: 2109 2124 2109 Size: 15 5659 SbfI CC_TGCA'GG Cuts at: 3026 3026 Size: 5674 ScaI AGT'ACT Cuts at: 2738 3922 5634 2738 Size: 1184 1712 2778 Fragments arranged by size: 2778 1712 1184 SexAI A'CCwGG_T Cuts at: 2085 2085 Size: 5674 SfcI C'TryA_G Cuts at: 826 1045 3022 4157 4838 5029 826 Size: 219 1977 1135 681 191 1471 Fragments arranged by size: 1977 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 2015 3001 1872 Size: 143 986 4545 Fragments arranged by size: 4545 986 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 2629 2780 3778 4679 4941 Size: 197 926 423 263 151 998 901 262 Cuts at: 4941 820 Size: 1553 Fragments arranged by size: 1553 998 926 901 423 263 262 197 151 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 2517 2867 3598 5 Size: 47 2465 350 731 2081 Fragments arranged by size: 2465 2081 731 350 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5674 StyI C'CwwG_G Cuts at: 513 1065 2004 3113 513 Size: 552 939 1109 3074 Fragments arranged by size: 3074 1109 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 3701 3718 3871 4056 4501 325 Size: 1255 546 1575 17 153 185 445 1498 Fragments arranged by size: 1575 1498 1255 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2736 3920 5632 Size: 274 80 33 51 1443 759 1184 1712 Cuts at: 5632 96 Size: 138 Fragments arranged by size: 1712 1443 1184 759 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5674 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5674 XmaI C'CCGG_G Cuts at: 1870 2013 2999 1870 Size: 143 986 4545 Fragments arranged by size: 4545 986 143 XmnI GAAnn'nnTTC Cuts at: 2673 2727 3803 2673 Size: 54 1076 4544 Fragments arranged by size: 4544 1076 54 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI Bsu36I DraIII EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI