################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MluI MslI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp45I TspRI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 516 TCCGGA T^CCGGA AflIII 317 ACATGT A^C[GA][CT]GT ApaI 848 GGGCCC GGGCC^C Asp700I 456 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 86 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 379 CCCGGG C^[CT]CG[GA]G BbrPI 919 CACGTG CAC^GTG BbvI 433 GCAGC GCAGC(8/12) BclI 176 TGATCA T^GATCA BglII 976 AGATCT A^GATCT BsaI 398 GAGACC GGTCTC(1/5) BsaWI 516 TCCGGA [AT]^CCGG[AT] BseAI 516 TCCGGA T^CCGGA BsmI 952 GAATGC GAATGC(1/-1) BsoBI 379 CCCGGG C^[CT]CG[GA]G BspDI 367 ATCGAT AT^CGAT BspEI 516 TCCGGA T^CCGGA BspLU11I 317 ACATGT A^CATGT BsrDI 913 GCAATG GCAATG(2/0) ClaI 367 ATCGAT AT^CGAT DraI 762 TTTAAA TTT^AAA DraII 1000 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 1000 GGGACCC [GA]G^G[ACGT]CC[CT] FauI 644 CCCGC CCCGC(4/6) FbaI 176 TGATCA T^GATCA Fnu4HI 433 GCAGC GC^[ACGT]GC HincII 667 GTCAAC GT[CT]^[GA]AC HindII 667 GTCAAC GT[CT]^[GA]AC ItaI 433 GCAGC GC^[ACGT]GC MaeIII 858 GTTAC ^GT[ACGT]AC MfeI 438 CAATTG C^AATTG MroI 516 TCCGGA T^CCGGA MunI 438 CAATTG C^AATTG MwoI 918 GCACGTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 317 ACATGT [GA]CATG^[CT] PflFI 86 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 919 CACGTG CAC^GTG PmlI 919 CACGTG CAC^GTG PpuMI 1000 GGGACCC [GA]G^G[AT]CC[CT] SmaI 379 CCCGGG CCC^GGG SnaBI 206 TACGTA TAC^GTA StuI 187 AGGCCT AGG^CCT TseI 433 GCAGC G^C[AT]GC Tth111I 86 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 72 CCATTTACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 379 CCCGGG C^CCGGG XmaI 379 CCCGGG C^CCGGG XmnI 456 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 793 GAATTT [GA]^AATT[CT] 808 GAATTT ApoI 793 GAATTT [GA]^AATT[CT] 808 GAATTT BalI 59 TGGCCA TGG^CCA 611 TGGCCA BanII 359 GGGCTC G[GA]GC[CT]^C 848 GGGCCC BmyI 359 GGGCTC G[AGT]GC[ACT]^C 848 GGGCCC BpmI 555 CTGGAG CTGGAG(16/14) 943 CTGGAG BsaAI 206 TACGTA [CT]AC^GT[GA] 919 CACGTG BseRI 653 GAGGAG GAGGAG(10/8) 1015 GAGGAG Bsp1286I 359 GGGCTC G[AGT]GC[ACT]^C 848 GGGCCC BstYI 513 AGATCC [GA]^GATC[CT] 976 AGATCT EaeI 59 TGGCCA [CT]^GGCC[GA] 611 TGGCCA EarI 14 CTCTTC CTCTTC(1/4) 821 CTCTTC Ksp632I 14 CTCTTC CTCTTC(1/4) 821 CTCTTC MflI 513 AGATCC [GA]^GATC[CT] 976 AGATCT MluNI 59 TGGCCA TGG^CCA 611 TGGCCA MscI 59 TGGCCA TGG^CCA 611 TGGCCA SfaNI 217 GATGC GCATC(5/9) 265 GATGC SfcI 101 CTATAG C^T[GA][CT]AG 873 CTACAG TfiI 417 GATTC G^A[AT]TC 939 GAATC XhoII 513 AGATCC [GA]^GATC[CT] 976 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BcnI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII FokI HaeIII HapII HinfI HpaII HphI MaeII MboI MboII MnlI MseI MspI MvaI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TaqI Tru9I Tsp509I TthHB8I