################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FspI HindIII HpaI KpnI KspI MamI MfeI MflI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AlwI 491 GGATC GGATC(4/5) ApaI 45 GGGCCC GGGCC^C AvaI 583 CCCGAG C^[CT]CG[GA]G AvaII 51 GGACC G^G[AT]CC BcgI 326 GCAAGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 60 GCTGAGC GC^T[ACGT]AGC Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC BsaAI 178 TACGTG [CT]AC^GT[GA] BseRI 781 CTCCTC GAGGAG(10/8) BsoBI 583 CCCGAG C^[CT]CG[GA]G BspHI 386 TCATGA T^CATGA BssSI 622 CTCGTG CACGAG(-5/-1) BstAPI 690 GCATGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 744 GGTGACC G^GT[ACGT]ACC BstPI 744 GGTGACC G^GT[ACGT]ACC CelII 60 GCTGAGC GC^T[ACGT]AGC DraII 719 AGGCCCT [GA]G^G[ACGT]CC[CT] EagI 444 CGGCCG C^GGCCG EarI 253 CTCTTC CTCTTC(1/4) EclXI 444 CGGCCG C^GGCCG Eco52I 444 CGGCCG C^GGCCG EcoO109I 719 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 744 GGTGACC G^GT[ACGT]ACC EcoT14I 716 CCAAGG C^C[AT][AT]GG FseI 441 GGCCGGCC GGCCGG^CC HincII 78 GTCGAC GT[CT]^[GA]AC HindII 78 GTCGAC GT[CT]^[GA]AC Ksp632I 253 CTCTTC CTCTTC(1/4) MaeII 179 ACGT A^CGT MluI 74 ACGCGT A^CGCGT NspI 690 GCATGC [GA]CATG^[CT] RcaI 386 TCATGA T^CATGA SalI 78 GTCGAC G^TCGAC SapI 252 GCTCTTC GCTCTTC(1/4) SexAI 404 ACCTGGT A^CC[AT]GGT SfaNI 40 GCATC GCATC(5/9) SfcI 453 CTACAG C^T[GA][CT]AG SphI 690 GCATGC GCATG^C StuI 587 AGGCCT AGG^CCT StyI 716 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 78 GTCGAC GT^[AC][GT]AC 127 GTCTAC AflIII 74 ACGCGT A^C[GA][CT]GT 179 ACGTGT BalI 236 TGGCCA TGG^CCA 416 TGGCCA BbsI 204 GAAGAC GAAGAC(2/6) 523 GTCTTC BfaI 535 CTAG C^TAG 568 CTAG BpmI 363 CTCCAG CTGGAG(16/14) 724 CTGGAG BpuAI 204 GAAGAC GAAGAC(2/6) 523 GTCTTC BsiEI 396 CGACCG CG[GA][CT]^CG 444 CGGCCG BstF5I 225 CATCC GGATG(2/0) 639 CATCC BstXI 695 CCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 716 CCAAGGCCCTGG DpnII 138 GATC ^GATC 492 GATC DsaI 146 CCGTGG C^C[GA][CT]GG 812 CCGTGG FokI 225 CATCC GGATG(9/13) 639 CATCC HinfI 81 GACTC G^A[ACGT]TC 427 GAGTC MaeI 535 CTAG C^TAG 568 CTAG MboI 138 GATC ^GATC 492 GATC MluNI 236 TGGCCA TGG^CCA 416 TGGCCA MscI 236 TGGCCA TGG^CCA 416 TGGCCA NdeII 138 GATC ^GATC 492 GATC PleI 81 GACTC GAGTC(4/5) 427 GAGTC PvuII 897 CAGCTG CAG^CTG 958 CAGCTG SacI 250 GAGCTC GAGCT^C 316 GAGCTC Sau3AI 138 GATC ^GATC 492 GATC TaqI 79 TCGA T^CGA 344 TCGA TthHB8I 79 TCGA T^CGA 344 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI BanI BanII BbeI BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV RsaI Sau96I ScrFI SfoI TseI Tsp45I TspRI