(Circular) (Six-base) MAPSORT of: pFN21AE9511.seq Check: 1973 from: 1 to: 5689 pFN21AE9511 5689 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2084 2133 3032 1937 Size: 147 49 899 4594 Fragments arranged by size: 4594 899 147 49 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2079 2184 4588 5565 1203 Size: 876 105 2404 977 1327 Fragments arranged by size: 2404 1327 977 876 105 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2267 2627 2819 1166 Size: 1101 360 192 4036 Fragments arranged by size: 4036 1101 360 192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaI G_GGCC'C Cuts at: 2054 2054 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2588 3014 1870 Size: 73 645 426 4545 Fragments arranged by size: 4545 645 426 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2092 2104 2197 2559 3010 Size: 325 217 627 305 12 93 362 451 Cuts at: 3010 4465 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 451 362 325 305 217 93 12 BanII G_rGCy'C Cuts at: 1391 2031 2054 2259 2325 3008 1391 Size: 640 23 205 66 683 4072 Fragments arranged by size: 4072 683 640 205 66 23 BbeI G_GCGC'C Cuts at: 2096 2108 2201 2563 2096 Size: 12 93 362 5222 Fragments arranged by size: 5222 362 93 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 2521 928 Size: 971 317 305 4096 Fragments arranged by size: 4096 971 317 305 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2320 2354 3880 3914 1903 Size: 34 383 34 1526 34 3678 Fragments arranged by size: 3678 1526 383 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 2066 3106 2066 Size: 1040 4649 Bme1580I G_kGCm'C Cuts at: 1790 2054 3750 4906 1790 Size: 264 1696 1156 2573 Fragments arranged by size: 2573 1696 1156 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2353 2750 4349 1239 Size: 1114 397 1599 2579 Fragments arranged by size: 2579 1599 1114 397 Bpu10I CC'TnA_GC Cuts at: 2810 2888 2810 Size: 78 5611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2185 3480 493 Size: 711 981 1295 2702 Fragments arranged by size: 2702 1295 981 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2093 2105 2198 2560 Size: 53 83 186 936 560 12 93 362 Cuts at: 2560 2798 2849 2937 3496 3878 4513 275 Size: 238 51 88 559 382 635 1451 Fragments arranged by size: 1451 936 635 560 559 382 362 238 186 93 88 83 53 51 12 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2312 2342 2351 2381 1178 Size: 30 165 30 909 30 9 30 4486 Fragments arranged by size: 4486 909 165 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2777 1186 Size: 1591 4098 BseYI C'CCAG_C Cuts at: 2632 4892 5388 2632 Size: 2260 496 2933 Fragments arranged by size: 2933 2260 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2404 2452 3139 3900 4928 1540 Size: 275 186 403 48 687 761 1028 2301 Fragments arranged by size: 2301 1028 761 687 403 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2259 2325 2816 3750 3835 4906 2259 Size: 66 491 934 85 1071 3042 Fragments arranged by size: 3042 1071 934 491 85 66 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2054 2259 2325 2816 3008 3750 Size: 399 241 23 205 66 491 192 742 Cuts at: 3750 3835 4906 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 491 399 241 205 192 85 66 23 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2391 3578 5308 2391 Size: 1187 1730 2772 Fragments arranged by size: 2772 1730 1187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 844 Size: 2200 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 2447 2556 2688 4333 4520 1700 Size: 110 291 346 109 132 1645 187 2869 Fragments arranged by size: 2869 1645 346 291 187 132 110 109 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 2468 2470 2605 2734 1925 Size: 543 2 135 129 4880 Fragments arranged by size: 4880 543 135 129 2 BssSI C'ACGA_G Cuts at: 2627 3749 4761 2627 Size: 1122 1012 3555 Fragments arranged by size: 3555 1122 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2701 794 Size: 1907 3782 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 2749 1184 Size: 1565 4124 BstXI CCAn_nnnn'nTGG Cuts at: 2707 2728 2707 Size: 21 5668 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 2817 513 Size: 552 939 147 666 3385 Fragments arranged by size: 3385 939 666 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2230 2269 2845 487 Size: 683 56 1004 39 576 3331 Fragments arranged by size: 3331 1004 683 576 56 39 BtsI GCAGTG_nn' Cuts at: 2312 2424 3205 3999 4019 2312 Size: 112 781 794 20 3982 Fragments arranged by size: 3982 794 781 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2421 2445 Size: 54 1425 50 275 18 411 180 24 Cuts at: 2445 2449 2484 3136 4025 5654 8 Size: 4 35 652 889 1629 43 Fragments arranged by size: 1629 1425 889 652 411 275 180 54 50 43 35 24 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2449 3136 1537 Size: 275 637 687 4090 Fragments arranged by size: 4090 687 637 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 3619 1353 Size: 911 1355 3423 Fragments arranged by size: 3423 1355 911 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2161 2948 4276 4650 4796 387 Size: 1064 138 572 787 1328 374 146 1280 Fragments arranged by size: 1328 1280 1064 787 572 374 146 138 EcoICRI GAG'CTC Cuts at: 2257 2323 2257 Size: 66 5623 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2353 2750 3752 4349 5136 5404 1239 Size: 393 721 397 1002 597 787 268 1524 Fragments arranged by size: 1524 1002 787 721 597 397 393 268 EcoO109I rG'GnC_Cy Cuts at: 2725 2725 Size: 5689 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FseI GG_CCGG'CC Cuts at: 2451 2451 Size: 5689 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2066 2096 2108 2201 2400 2466 2563 2828 Size: 388 30 12 93 199 66 97 265 Cuts at: 2828 4836 1678 Size: 2008 2531 Fragments arranged by size: 2531 2008 388 265 199 97 93 66 30 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 4330 4362 4404 Size: 32 135 32 428 32 2298 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 2298 428 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2085 3033 3279 669 Size: 988 281 147 948 246 3079 Fragments arranged by size: 3079 988 948 281 246 147 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2092 2104 2197 2559 2092 Size: 12 93 362 5222 Fragments arranged by size: 5222 362 93 12 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 2079 5565 2079 Size: 3486 2203 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 4803 4987 1305 Size: 795 2703 184 2007 Fragments arranged by size: 2703 2007 795 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2243 2423 10 Size: 54 1425 343 411 180 3276 Fragments arranged by size: 3276 1425 411 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2390 2627 2648 2819 3647 4006 Size: 648 1101 123 237 21 171 828 359 Cuts at: 4006 4165 518 Size: 159 2042 Fragments arranged by size: 2042 1101 828 648 359 237 171 159 123 21 MspA1I CmG'CkG Cuts at: 1606 2098 2110 2854 2904 2965 3105 3782 4930 Size: 492 12 744 50 61 140 677 1148 Cuts at: 4930 5175 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 744 677 492 245 140 61 50 12 NaeI GCC'GGC Cuts at: 2103 2449 2558 2690 2103 Size: 346 109 132 5102 Fragments arranged by size: 5102 346 132 109 NarI GG'CG_CC Cuts at: 2093 2105 2198 2560 2093 Size: 12 93 362 5222 Fragments arranged by size: 5222 362 93 12 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2101 2447 2556 2688 2101 Size: 346 109 132 5102 Fragments arranged by size: 5102 346 132 109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2699 3047 4592 1132 Size: 97 1470 348 1545 2229 Fragments arranged by size: 2229 1545 1470 348 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PfoI T'CCnGG_A Cuts at: 2931 2931 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2050 2050 Size: 5689 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2904 2965 2904 Size: 61 5628 SacI G_AGCT'C Cuts at: 2259 2325 2259 Size: 66 5623 SalI G'TCGA_C Cuts at: 1936 2083 3031 1936 Size: 147 948 4594 Fragments arranged by size: 4594 948 147 SapI GCTCTTCn'nnn_ Cuts at: 2264 2264 Size: 5689 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SexAI A'CCwGG_T Cuts at: 2409 2409 Size: 5689 SfcI C'TryA_G Cuts at: 826 1045 2458 3037 4172 4853 5044 826 Size: 219 1413 579 1135 681 191 1471 Fragments arranged by size: 1471 1413 1135 681 579 219 191 SfoI GGC'GCC Cuts at: 2094 2106 2199 2561 2094 Size: 12 93 362 5222 Fragments arranged by size: 5222 362 93 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3793 4694 4956 820 Size: 197 926 1850 901 262 1553 Fragments arranged by size: 1850 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 2699 3047 1132 Size: 1567 348 3774 Fragments arranged by size: 3774 1567 348 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2594 1517 Size: 1077 4612 StyI C'CwwG_G Cuts at: 513 1065 2004 2721 3128 513 Size: 552 939 717 407 3074 Fragments arranged by size: 3074 939 717 552 407 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3716 3733 3886 4071 4516 325 Size: 1255 2136 17 153 185 445 1498 Fragments arranged by size: 2136 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 3935 5647 Size: 274 80 33 51 1443 378 1580 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1580 1443 378 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2312 2344 5649 5681 2312 Size: 32 3305 32 2320 Fragments arranged by size: 3305 2320 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI