################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BglII BlnI BsaI BspHI BspLU11I BssSI Bst1107I BstBI BstEII BstPI BstZ17I CpoI Eam1105I Eco47III EcoO65I EcoRI EcoRV FseI HpaI KasI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NheI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SapI ScaI SfiI SfoI SfuI SgrAI SpeI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### Acc65I 1537 GGTACC G^GTACC AccIII 1071 TCCGGA T^CCGGA AflII 1345 CTTAAG C^TTAAG AocI 1957 CCTCAGG CC^T[ACGT]AGG Asp718I 1537 GGTACC G^GTACC AviII 189 TGCGCA TGC^GCA BanI 1537 GGTACC G^G[CT][GA]CC BfrI 1345 CTTAAG C^TTAAG BlpI 2216 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2216 GCTTAGC GC^T[ACGT]AGC BsaBI 1509 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1071 TCCGGA [AT]^CCGG[AT] BseAI 1071 TCCGGA T^CCGGA BsiEI 957 CGGCCG CG[GA][CT]^CG BsiWI 1634 CGTACG C^GTACG BsmBI 105 GAGACG CGTCTC(1/5) BsmI 225 GCATTC GAATGC(1/-1) BspDI 1717 ATCGAT AT^CGAT BspEI 1071 TCCGGA T^CCGGA BspMI 173 ACCTGC ACCTGC(4/8) BsrBI 1026 CCGCTC CCGCTC(-3/-3) BsrFI 38 GCCGGC [GA]^CCGG[CT] BssHII 1825 GCGCGC G^CGCGC BstXI 2264 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 381 GGATCT [GA]^GATC[CT] Bsu36I 1957 CCTCAGG CC^T[ACGT]AGG CelII 2216 GCTTAGC GC^T[ACGT]AGC Cfr10I 38 GCCGGC [GA]^CCGG[CT] ClaI 1717 ATCGAT AT^CGAT DraI 621 TTTAAA TTT^AAA DraII 368 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 2528 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 712 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 957 CGGCCG [CT]^GGCC[GA] EagI 957 CGGCCG C^GGCCG EclXI 957 CGGCCG C^GGCCG Eco52I 957 CGGCCG C^GGCCG Eco81I 1957 CCTCAGG CC^T[ACGT]AGG EcoNI 1970 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 368 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 1394 CCTTGG C^C[AT][AT]GG EcoT22I 223 ATGCAT ATGCA^T FspI 189 TGCGCA TGC^GCA HaeII 986 GGCGCT [GA]GCGC^[CT] KpnI 1537 GGTACC GGTAC^C MamI 1509 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 381 GGATCT [GA]^GATC[CT] MroI 1071 TCCGGA T^CCGGA NaeI 38 GCCGGC GCC^GGC NgoMIV 38 GCCGGC G^CCGGC NotI 956 GCGGCCGC GC^GGCCGC NsiI 223 ATGCAT ATGCA^T PshAI 1975 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2038 GAGCTC GAGCT^C SalI 15 GTCGAC G^TCGAC SbfI 1084 CCTGCAGG CCTGCA^GG SexAI 1994 ACCTGGT A^CC[AT]GGT SmaI 938 CCCGGG CCC^GGG SmlI 1345 CTTAAG C^T[CT][GA]AG SnaBI 1636 TACGTA TAC^GTA SphI 481 GCATGC GCATG^C Sse8387I 1084 CCTGCAGG CCTGCA^GG StyI 1394 CCTTGG C^C[AT][AT]GG XhoII 381 GGATCT [GA]^GATC[CT] XmaCI 938 CCCGGG C^CCGGG XmaI 938 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AseI 598 ATTAAT AT^TAAT 2208 ATTAAT AsnI 598 ATTAAT AT^TAAT 2208 ATTAAT AspHI 1682 GTGCTC G[AT]GC[AT]^C 2038 GAGCTC AspI 1138 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1782 GACTCTGTC BclI 866 TGATCA T^GATCA 1052 TGATCA BfaI 436 CTAG C^TAG 2200 CTAG BpmI 1611 CTCCAG CTGGAG(16/14) 164 CTGGAG BsaAI 1564 TACGTG [CT]AC^GT[GA] 1636 TACGTA BsgI 359 CTGCAC GTGCAG(16/14) 1616 GTGCAG BsiHKAI 1682 GTGCTC G[AT]GC[AT]^C 2038 GAGCTC Bsp1407I 665 TGTACA T^GTACA 1568 TGTACA BsrGI 665 TGTACA T^GTACA 1568 TGTACA EarI 1033 CTCTTC CTCTTC(1/4) 2275 GAAGAG FbaI 866 TGATCA T^GATCA 1052 TGATCA HincII 15 GTCGAC GT[CT]^[GA]AC 1135 GTTGAC HindII 15 GTCGAC GT[CT]^[GA]AC 1135 GTTGAC HindIII 1057 AAGCTT A^AGCTT 2419 AAGCTT Ksp632I 1033 CTCTTC CTCTTC(1/4) 2275 GAAGAG MaeI 436 CTAG C^TAG 2200 CTAG PflFI 1138 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1782 GACTCTGTC PflMI 307 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1002 CCACAGCATGG PshBI 598 ATTAAT AT^TAAT 2208 ATTAAT SspBI 665 TGTACA T^GTACA 1568 TGTACA StuI 343 AGGCCT AGG^CCT 902 AGGCCT Tth111I 1138 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1782 GACTCTGTC Van91I 307 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1002 CCACAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AvaI AvaII BanII BbsI BbvI BcnI BglI BmyI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI