Restriction map for
pFN21AE5321
(Circular) (Six-base) MAPSORT of: pFN21AE5321.seq Check: 896 from: 1 to: 5554
pFN21AE5321 5554 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 2791 3617 5001 5269 1632
Size: 669 490 826 1384 268 1917
Fragments arranged by size:
1917 1384 826 669 490 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 2551 4453 5430 1203
Size: 1348 1902 977 1327
Fragments arranged by size:
1902 1348 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 2765 4283 2765
Size: 1518 4036
AleI CACnn'nnGTG
Cuts at: 1166 2552 1166
Size: 1386 4168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AlwNI CAG_nnn'CTG
Cuts at: 3297 4869 3297
Size: 1572 3982
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2123 2570 2863 3089 3241 5350 1493
Size: 630 447 293 226 152 2109 1697
Fragments arranged by size:
2109 1697 630 447 293 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2879 1870
Size: 73 936 4545
Fragments arranged by size:
4545 936 73
AvrII C'CTAG_G
Cuts at: 2032 2032
Size: 5554
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2875 4330 618
Size: 325 217 627 1088 1455 1842
Fragments arranged by size:
1842 1455 1088 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2873 1391
Size: 1482 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2047 928
Size: 971 148 4435
Fragments arranged by size:
4435 971 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2971 2971
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 1790 3615 4771 1790
Size: 1825 1156 2573
Fragments arranged by size:
2573 1825 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2747 3285 4243
Size: 416 53 83 399 24 1330 538 958
Cuts at: 4243 442
Size: 1753
Fragments arranged by size:
1753 1330 958 538 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4214 1239
Size: 2975 2579
Bpu10I CC'TnA_GC
Cuts at: 2046 2046
Size: 5554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3679 4544 4842 3679
Size: 865 298 4391
Fragments arranged by size:
4391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3345 493
Size: 711 2141 2702
Fragments arranged by size:
2702 2141 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3361 3743 4378 275
Size: 53 83 186 936 1828 382 635 1451
Fragments arranged by size:
1828 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 1178
Size: 30 165 30 855 30 4444
Fragments arranged by size:
4444 855 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5554
BseYI C'CCAG_C
Cuts at: 2424 2639 4757 5253 2424
Size: 215 2118 496 2725
Fragments arranged by size:
2725 2118 496 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 2832 1163
Size: 573 792 304 3885
Fragments arranged by size:
3885 792 573 304
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3615 3700 4771 3615
Size: 85 1071 4398
Fragments arranged by size:
4398 1071 85
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2873 3615 3700 4771 1391
Size: 399 1083 742 85 1071 2174
Fragments arranged by size:
2174 1083 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 844
Size: 2065 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 1394
Size: 429 1618 3507
Fragments arranged by size:
3507 1618 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2664 4049 4223 65
Size: 1183 1416 1385 174 1396
Fragments arranged by size:
1416 1396 1385 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4198 4385 1700
Size: 110 2388 187 2869
Fragments arranged by size:
2869 2388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 2442 4358 2442
Size: 1916 3638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstYI r'GATC_y
Cuts at: 1972 2884 3251 3636 3653 5105 5191 5203 5549
Size: 912 367 385 17 1452 86 12 346
Cuts at: 5549 1972
Size: 1977
Fragments arranged by size:
1977 1452 912 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5002
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 3864 3884 3070
Size: 794 20 4740
Fragments arranged by size:
4740 794 20
ClaI AT'CG_AT
Cuts at: 1554 2793 1554
Size: 1239 4315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3001 3890 5519
Size: 54 1425 50 275 18 1171 889 1629
Cuts at: 5519 8
Size: 43
Fragments arranged by size:
1629 1425 1171 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 3484 1353
Size: 974 1157 3423
Fragments arranged by size:
3423 1157 974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2791 3617 4214 5001 5269 1239
Size: 393 669 490 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 669 597 490 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2036
Size: 5554
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2468 2848 2071
Size: 397 380 4777
Fragments arranged by size:
4777 397 380
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 4701 1678
Size: 3023 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2432 2568 2600 2751
Size: 32 135 32 828 32 136 32 151
Cuts at: 2751 2783 4195 4227 4269 4301 1373
Size: 32 1412 32 42 32 2626
Fragments arranged by size:
2626 1412 828 151 136 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3732
Fragments arranged by size:
3732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2550 2552 3512 3871 4030 518
Size: 648 1384 2 960 359 159 2042
Fragments arranged by size:
2042 1384 960 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2542 2639 2970 3647 4795 5040 1606
Size: 936 97 331 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 677 331 245 97
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5002
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2555 2683 2912 4457 1132
Size: 97 1326 128 229 1545 2229
Fragments arranged by size:
2229 1545 1326 229 128 97
PasI CC'CwG_GG
Cuts at: 1348 2515 1348
Size: 1167 4387
PciI A'CATG_T
Cuts at: 2551 4453 2551
Size: 1902 3652
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2906 830
Size: 2076 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
PvuII CAG'CTG
Cuts at: 2542 2639 2542
Size: 97 5457
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2902 4037 4718 4909 826
Size: 219 1857 1135 681 191 1471
Fragments arranged by size:
1857 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2881 1872
Size: 1009 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3658 4559 4821 820
Size: 197 926 1715 901 262 1553
Fragments arranged by size:
1715 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 2351 1517
Size: 834 4720
StyI C'CwwG_G
Cuts at: 513 1065 2032 2993 513
Size: 552 967 961 3074
Fragments arranged by size:
3074 967 961 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2234 3581 3598 3751 3936 4381 325
Size: 1255 654 1347 17 153 185 445 1498
Fragments arranged by size:
1498 1347 1255 654 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2676 3800 5512
Size: 274 80 33 51 1443 699 1124 1712
Cuts at: 5512 96
Size: 138
Fragments arranged by size:
1712 1443 1124 699 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5514 5546 5514
Size: 32 5522
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2879 1870
Size: 1009 4545
XmnI GAAnn'nnTTC
Cuts at: 2507 2804 3683 2507
Size: 297 879 4378
Fragments arranged by size:
4378 879 297
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI